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The map label for this gene is smpB

Identifier: 45658263

GI number: 45658263

Start: 2932356

End: 2932838

Strand: Reverse

Name: smpB

Synonym: LIC12418

Alternate gene names: 45658263

Gene position: 2932838-2932356 (Counterclockwise)

Preceding gene: 45658265

Following gene: 45658262

Centisome position: 68.57

GC content: 35.2

Gene sequence:

>483_bases
ATGGTAAAGAAAAAAGAAGAATCTGGGCATTCCCCGCTAGTCAATAAAAAGGCAAAGTTCAACTTCGAATTAATTTCATT
TCTAGAGGCGGGGATCGTATTGTCCGGATCCGAAGTTAAAAGTCTACGTGATAAAAAAGGAAATCTAACAGACGCATTTG
CGAAGATCAAAAATGGAGAAGTGTTTTTAGAAAATTTTTCTATTGCTCCCTATAAAAACGGAGGGTATGCAAATCATCCA
GAAATCAGACCTAGAAAGCTTTTATTACATAAGAAGGAAATTGAAAAGTTAGAACGTCAAGTAAAGGAGAAGGGTTTGGT
TTTAGTTGCCACTAAAGTTTATTTTAAAAATAATCTTAGGGTCAAAGTGGAAATTGCAGTAGGTAAGCCTAAAAAGTTAC
ACGATAAACGGGACGATTTACAAAAAAAAGACGCTCAACAAGAAATTGCACGCGCATTGAAATCATCCAATCGTTATCAA
TGA

Upstream 100 bases:

>100_bases
TCAATTTTTTAAGAGTTTTTATAAAGGCTGCATAACGGTGCTTTGGAAAAAATTTTTTTCCTGAATTGATACAAATTAAA
CCGCTGGTTTCATAAAAAAT

Downstream 100 bases:

>100_bases
ACGACCGCAAAAATTAGGATTAAGATGGCTAAAGCCGTAAAAAAAGAAATCGCAAAATCGGAAGAAGTCGTTTCGATCAA
AGCCCCTCGTAAAGAACCTG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MVKKKEESGHSPLVNKKAKFNFELISFLEAGIVLSGSEVKSLRDKKGNLTDAFAKIKNGEVFLENFSIAPYKNGGYANHP
EIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLRVKVEIAVGKPKKLHDKRDDLQKKDAQQEIARALKSSNRYQ

Sequences:

>Translated_160_residues
MVKKKEESGHSPLVNKKAKFNFELISFLEAGIVLSGSEVKSLRDKKGNLTDAFAKIKNGEVFLENFSIAPYKNGGYANHP
EIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLRVKVEIAVGKPKKLHDKRDDLQKKDAQQEIARALKSSNRYQ
>Mature_160_residues
MVKKKEESGHSPLVNKKAKFNFELISFLEAGIVLSGSEVKSLRDKKGNLTDAFAKIKNGEVFLENFSIAPYKNGGYANHP
EIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLRVKVEIAVGKPKKLHDKRDDLQKKDAQQEIARALKSSNRYQ

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=37.2670807453416, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18285; Mature: 18285

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKKEESGHSPLVNKKAKFNFELISFLEAGIVLSGSEVKSLRDKKGNLTDAFAKIKNGE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEECCHHHHHHHHCCCCHHHHHHHHCCCE
VFLENFSIAPYKNGGYANHPEIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLR
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCE
VKVEIAVGKPKKLHDKRDDLQKKDAQQEIARALKSSNRYQ
EEEEEEECCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVKKKEESGHSPLVNKKAKFNFELISFLEAGIVLSGSEVKSLRDKKGNLTDAFAKIKNGE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEECCHHHHHHHHCCCCHHHHHHHHCCCE
VFLENFSIAPYKNGGYANHPEIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLR
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCE
VKVEIAVGKPKKLHDKRDDLQKKDAQQEIARALKSSNRYQ
EEEEEEECCCHHHHCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA