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The map label for this gene is tpiA

Identifier: 45657942

GI number: 45657942

Start: 2533875

End: 2534627

Strand: Direct

Name: tpiA

Synonym: LIC12094

Alternate gene names: 45657942

Gene position: 2533875-2534627 (Clockwise)

Preceding gene: 45657939

Following gene: 45657943

Centisome position: 59.24

GC content: 37.32

Gene sequence:

>753_bases
ATGCGTAAGACAATTATTGCAGGAAACTGGAAAATGAATTTATCTCTTAAAGAAGCCGTTTTCCTAGCACATTCGATCCG
AGAAAAAATTCCATCCATTTCTAAAGATAAAGTCTCGATGGTATTTCCTTCTACTCTTCATCTTGAAAACGTTTCCAAAA
TATTAGAAGGTAGCAGTGTAATTGTTGGAGCCCAAAACTGTTATCACTCCGGACTTGCAGCTTTTACCGGCGAAACCTCT
CCCGATCAACTGAAAGAAATTGGTGTTAAAGTTGTAATGGTCGGTCATTCGGAACGTCGTCAATTTTTAGGAGAGTCTAA
CTTTTTTTGTAACGATAAAATCCGTTTTCTTTTAAAGAACGAATTTACAGTCCTTTATTGTGTTGGAGAAACTCTCTCCG
AAAGAGAATCCGGTAAAACATTGGAAGTACTGTCTTCTCAAATTAGAGAAGGTTTAAAAGGAATTGATAGCGTATTTTTT
TCCAATTTAATTCTGGCTTATGAACCAGTTTGGGCCATTGGAACCGGAAAAGTTGCCACCCCCTCTCAAGCCCAAGAAGT
ACATTCTTTTATTCGTAAAGAAATTTCTGGTCTTTTTGTAGGCGCCTCTTCTATTTCAGAATCCATTTCTATTCTCTACG
GTGGTTCTGTAAAACCAGACAATATTCAAGATCTATTAAAAGAAAAAGATATAGATGGGGGTTTAGTTGGAGGAGCCAGC
CAAAAAATCAGTTCCTTCGCTGGACTTTTCTAA

Upstream 100 bases:

>100_bases
TTTTAAATTCGATCAAAAGTTTCAAAAATTCTTGTAGGGATTTCCCTTTCCTAAAAACTTTTCCCAGTTCAATTGTACTC
ATCTTTCAAACGAGGAAATT

Downstream 100 bases:

>100_bases
ACTCGAATTGGAAAACACTTCAGATGAGTATTTTAATTGTCACTCTCTGAAGGATTCATAATCTGGCAAAGGATTCATTT
TTTTTCAGGTATTTATATGT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MRKTIIAGNWKMNLSLKEAVFLAHSIREKIPSISKDKVSMVFPSTLHLENVSKILEGSSVIVGAQNCYHSGLAAFTGETS
PDQLKEIGVKVVMVGHSERRQFLGESNFFCNDKIRFLLKNEFTVLYCVGETLSERESGKTLEVLSSQIREGLKGIDSVFF
SNLILAYEPVWAIGTGKVATPSQAQEVHSFIRKEISGLFVGASSISESISILYGGSVKPDNIQDLLKEKDIDGGLVGGAS
QKISSFAGLF

Sequences:

>Translated_250_residues
MRKTIIAGNWKMNLSLKEAVFLAHSIREKIPSISKDKVSMVFPSTLHLENVSKILEGSSVIVGAQNCYHSGLAAFTGETS
PDQLKEIGVKVVMVGHSERRQFLGESNFFCNDKIRFLLKNEFTVLYCVGETLSERESGKTLEVLSSQIREGLKGIDSVFF
SNLILAYEPVWAIGTGKVATPSQAQEVHSFIRKEISGLFVGASSISESISILYGGSVKPDNIQDLLKEKDIDGGLVGGAS
QKISSFAGLF
>Mature_250_residues
MRKTIIAGNWKMNLSLKEAVFLAHSIREKIPSISKDKVSMVFPSTLHLENVSKILEGSSVIVGAQNCYHSGLAAFTGETS
PDQLKEIGVKVVMVGHSERRQFLGESNFFCNDKIRFLLKNEFTVLYCVGETLSERESGKTLEVLSSQIREGLKGIDSVFF
SNLILAYEPVWAIGTGKVATPSQAQEVHSFIRKEISGLFVGASSISESISILYGGSVKPDNIQDLLKEKDIDGGLVGGAS
QKISSFAGLF

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=40.1639344262295, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=40.1639344262295, Blast_Score=166, Evalue=1e-41,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=42.1052631578947, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=241, Percent_Identity=41.0788381742739, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=244, Percent_Identity=40.9836065573771, Blast_Score=173, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28572008, Length=241, Percent_Identity=41.9087136929461, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572006, Length=241, Percent_Identity=41.9087136929461, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572004, Length=241, Percent_Identity=41.9087136929461, Blast_Score=177, Evalue=7e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_LEPIC (Q72QL9)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002028.1
- ProteinModelPortal:   Q72QL9
- SMR:   Q72QL9
- GeneID:   2769544
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12094
- NMPDR:   fig|267671.1.peg.2028
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   LINT267671:LIC_12094-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27238; Mature: 27238

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 168-168 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTIIAGNWKMNLSLKEAVFLAHSIREKIPSISKDKVSMVFPSTLHLENVSKILEGSSV
CCCEEEECCEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCEE
IVGAQNCYHSGLAAFTGETSPDQLKEIGVKVVMVGHSERRQFLGESNFFCNDKIRFLLKN
EEECHHHHHCCHHHHCCCCCHHHHHHCCEEEEEECCHHHHHHCCCCCCEECCHHEEEEEC
EFTVLYCVGETLSERESGKTLEVLSSQIREGLKGIDSVFFSNLILAYEPVWAIGTGKVAT
CCEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PSQAQEVHSFIRKEISGLFVGASSISESISILYGGSVKPDNIQDLLKEKDIDGGLVGGAS
CHHHHHHHHHHHHHHHHEEEEHHHHHCCEEEEECCCCCCCHHHHHHHHCCCCCCEECCHH
QKISSFAGLF
HHHHHHHCCC
>Mature Secondary Structure
MRKTIIAGNWKMNLSLKEAVFLAHSIREKIPSISKDKVSMVFPSTLHLENVSKILEGSSV
CCCEEEECCEEEECHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCEE
IVGAQNCYHSGLAAFTGETSPDQLKEIGVKVVMVGHSERRQFLGESNFFCNDKIRFLLKN
EEECHHHHHCCHHHHCCCCCHHHHHHCCEEEEEECCHHHHHHCCCCCCEECCHHEEEEEC
EFTVLYCVGETLSERESGKTLEVLSSQIREGLKGIDSVFFSNLILAYEPVWAIGTGKVAT
CCEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PSQAQEVHSFIRKEISGLFVGASSISESISILYGGSVKPDNIQDLLKEKDIDGGLVGGAS
CHHHHHHHHHHHHHHHHEEEEHHHHHCCEEEEECCCCCCCHHHHHHHHCCCCCCEECCHH
QKISSFAGLF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA