Definition Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession NC_005823
Length 4,277,185

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The map label for this gene is cysS

Identifier: 45657880

GI number: 45657880

Start: 2445368

End: 2446783

Strand: Direct

Name: cysS

Synonym: LIC12028

Alternate gene names: 45657880

Gene position: 2445368-2446783 (Clockwise)

Preceding gene: 45657879

Following gene: 45657881

Centisome position: 57.17

GC content: 36.58

Gene sequence:

>1416_bases
ATGATAGAAATCCAATTTTACAATTCACTTTCGGGTAGAAAGGAAAAATTCTCCCCTTCCGATCCAAATCGTGTAACCGT
ATATTCCTGCGGACCAACTGTTTATAATTTTGCTCATATAGGAAATCTACGAGCGTTTCTTTTCGTAGATGTTTTACGTA
GATCCCTCAAGTTATTAGGATATGGAGTGGATATGACTATGAACATCACCGATATAGACGACAAGATCATCCGTGATTCA
ATTGCATCTAAAAAAAGCATCATAGAATTTACAGCACCTTGGACAAAAGCATTCTTCGAAGATTTAAAAACAGTTTCTGC
TGAAATTTTAGAACACTATCCCAAGGCAACAGATTCTATTCCTGAAATGGTAGACATCATTCAAAAACTACAAAAAAAAG
GGCTCGTCTACGAAAAAGACGAAAGCCTTTATTTTTCGATCCAGAAATTTCAAAACTACGGAAAACTCAGTAAGATAGAT
ACTTCCGGAATGAAAACAGGTACTCGTTATGATACCGACGAATACGAAAAAGAAGACGTAAGAGACTTCGTTCTTTGGAA
AAGTCCTAAATTAGAAGGGGAAACTTCTTGGTATACGTTAGTCGGAACTGGTCGCCCAGGATGGCATTTAGAATGTTCTG
CAATGATTCGAAAAGTATATGGAAGTGGAGTAGACATTCATACCGGCGGGGTAGATCTTTTATTCCCGCATCATGAAAAT
GAAATCGCACAATCCGAAGGAGCCTTTCCAGAAGAATCTTTTGTAAAAACATGGCTGCATTCTGAGCACCTTCTCGTGGA
CGGTCAAAAAATGTCCAAGAGCAAAGGAAACTTTTACACGTTACGCGATCTTATTCAACAAGGTTTAGATCCTAAAGCGA
TCCGTTTTCTTTTGATCTCTACTCATTATCGTTCTAAGCTAAATTTTTCAACCGATAGAATCGCTGAAGCTTCCGCAAAT
ATTCGTAAAATCCAAAATTGTTTAGATCGAATTTTAGAATTAGAACCGGATATAAAAGCGGATTTTTACTTTCTATTTTC
GATCCCATTTGTACAAACTTGGAAAAAAGAATTTGAAGAATCTTTGGCAGACGACTTAAATATTTCCAAAGCCTTGGCGG
TTGTTTTCGAATCGGTAAAACAAATCAATTCCTTGTTGGATACAAACCAGTCCGATTCCAAACAAAGAATAGAATATATT
CAAATTCTTGCATATTTTGATCGTATTTTAGGAGTTCTCAATTTTGAATCTAAAAAGGATTTGCTCGATTCGGAAATCGA
TTCTCTGATCGAAGAAAGACAAATTGCCAGAAAAAATAAGGATTTTGCAAGATCCGACGCAATCCGGGATCAACTTTTAG
CACAAGGAATTTTGATAGAGGATACAAAAGAAGGAATCCGCTGGAGGAAAAAATAG

Upstream 100 bases:

>100_bases
GAATGCAAGTTTATCCTACAGAAGGAAACGAAGCAGGTTTCAAAGACGAAAAACAAAATGGTGCCAACCTGGAATTTGTG
AGGGAAATTTTCTTTCCCGA

Downstream 100 bases:

>100_bases
CTAAGCCCGAATACATTTTTGGAAAGAGGACTCTGATTGAACTTACGGAGGCCAATCTAGGAAAGGAACATTCATATCCT
TTTATCCAATTGTATATAAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MIEIQFYNSLSGRKEKFSPSDPNRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLGYGVDMTMNITDIDDKIIRDS
IASKKSIIEFTAPWTKAFFEDLKTVSAEILEHYPKATDSIPEMVDIIQKLQKKGLVYEKDESLYFSIQKFQNYGKLSKID
TSGMKTGTRYDTDEYEKEDVRDFVLWKSPKLEGETSWYTLVGTGRPGWHLECSAMIRKVYGSGVDIHTGGVDLLFPHHEN
EIAQSEGAFPEESFVKTWLHSEHLLVDGQKMSKSKGNFYTLRDLIQQGLDPKAIRFLLISTHYRSKLNFSTDRIAEASAN
IRKIQNCLDRILELEPDIKADFYFLFSIPFVQTWKKEFEESLADDLNISKALAVVFESVKQINSLLDTNQSDSKQRIEYI
QILAYFDRILGVLNFESKKDLLDSEIDSLIEERQIARKNKDFARSDAIRDQLLAQGILIEDTKEGIRWRKK

Sequences:

>Translated_471_residues
MIEIQFYNSLSGRKEKFSPSDPNRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLGYGVDMTMNITDIDDKIIRDS
IASKKSIIEFTAPWTKAFFEDLKTVSAEILEHYPKATDSIPEMVDIIQKLQKKGLVYEKDESLYFSIQKFQNYGKLSKID
TSGMKTGTRYDTDEYEKEDVRDFVLWKSPKLEGETSWYTLVGTGRPGWHLECSAMIRKVYGSGVDIHTGGVDLLFPHHEN
EIAQSEGAFPEESFVKTWLHSEHLLVDGQKMSKSKGNFYTLRDLIQQGLDPKAIRFLLISTHYRSKLNFSTDRIAEASAN
IRKIQNCLDRILELEPDIKADFYFLFSIPFVQTWKKEFEESLADDLNISKALAVVFESVKQINSLLDTNQSDSKQRIEYI
QILAYFDRILGVLNFESKKDLLDSEIDSLIEERQIARKNKDFARSDAIRDQLLAQGILIEDTKEGIRWRKK
>Mature_471_residues
MIEIQFYNSLSGRKEKFSPSDPNRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLGYGVDMTMNITDIDDKIIRDS
IASKKSIIEFTAPWTKAFFEDLKTVSAEILEHYPKATDSIPEMVDIIQKLQKKGLVYEKDESLYFSIQKFQNYGKLSKID
TSGMKTGTRYDTDEYEKEDVRDFVLWKSPKLEGETSWYTLVGTGRPGWHLECSAMIRKVYGSGVDIHTGGVDLLFPHHEN
EIAQSEGAFPEESFVKTWLHSEHLLVDGQKMSKSKGNFYTLRDLIQQGLDPKAIRFLLISTHYRSKLNFSTDRIAEASAN
IRKIQNCLDRILELEPDIKADFYFLFSIPFVQTWKKEFEESLADDLNISKALAVVFESVKQINSLLDTNQSDSKQRIEYI
QILAYFDRILGVLNFESKKDLLDSEIDSLIEERQIARKNKDFARSDAIRDQLLAQGILIEDTKEGIRWRKK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=484, Percent_Identity=30.3719008264463, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI303304987, Length=236, Percent_Identity=35.1694915254237, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI21269877, Length=236, Percent_Identity=35.1694915254237, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI62240992, Length=236, Percent_Identity=35.1694915254237, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI10835051, Length=236, Percent_Identity=35.1694915254237, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1786737, Length=481, Percent_Identity=36.1746361746362, Blast_Score=285, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17509797, Length=251, Percent_Identity=33.0677290836653, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17509795, Length=251, Percent_Identity=33.0677290836653, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324082, Length=370, Percent_Identity=31.0810810810811, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI19922218, Length=513, Percent_Identity=28.0701754385965, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI19922364, Length=325, Percent_Identity=32, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_LEPIC (Q72QT1)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001966.1
- ProteinModelPortal:   Q72QT1
- SMR:   Q72QT1
- GeneID:   2771233
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12028
- NMPDR:   fig|267671.1.peg.1966
- HOGENOM:   HBG327651
- OMA:   HHENERI
- ProtClustDB:   PRK00260
- BioCyc:   LINT267671:LIC_12028-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54311; Mature: 54311

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MIEIQFYNSLSGRKEKFSPSDPNRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLG
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHC
YGVDMTMNITDIDDKIIRDSIASKKSIIEFTAPWTKAFFEDLKTVSAEILEHYPKATDSI
CCCCEEEEEECCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
PEMVDIIQKLQKKGLVYEKDESLYFSIQKFQNYGKLSKIDTSGMKTGTRYDTDEYEKEDV
HHHHHHHHHHHHCCCEEECCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RDFVLWKSPKLEGETSWYTLVGTGRPGWHLECSAMIRKVYGSGVDIHTGGVDLLFPHHEN
HHHHHCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCEEECCCEEEEECCCCC
EIAQSEGAFPEESFVKTWLHSEHLLVDGQKMSKSKGNFYTLRDLIQQGLDPKAIRFLLIS
HHHHHCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHEE
THYRSKLNFSTDRIAEASANIRKIQNCLDRILELEPDIKADFYFLFSIPFVQTWKKEFEE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHH
SLADDLNISKALAVVFESVKQINSLLDTNQSDSKQRIEYIQILAYFDRILGVLNFESKKD
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LLDSEIDSLIEERQIARKNKDFARSDAIRDQLLAQGILIEDTKEGIRWRKK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCHHCCCCCCC
>Mature Secondary Structure
MIEIQFYNSLSGRKEKFSPSDPNRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLG
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHC
YGVDMTMNITDIDDKIIRDSIASKKSIIEFTAPWTKAFFEDLKTVSAEILEHYPKATDSI
CCCCEEEEEECCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
PEMVDIIQKLQKKGLVYEKDESLYFSIQKFQNYGKLSKIDTSGMKTGTRYDTDEYEKEDV
HHHHHHHHHHHHCCCEEECCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RDFVLWKSPKLEGETSWYTLVGTGRPGWHLECSAMIRKVYGSGVDIHTGGVDLLFPHHEN
HHHHHCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHCCCCEEECCCEEEEECCCCC
EIAQSEGAFPEESFVKTWLHSEHLLVDGQKMSKSKGNFYTLRDLIQQGLDPKAIRFLLIS
HHHHHCCCCCHHHHHHHHHCCCCEEECCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHEE
THYRSKLNFSTDRIAEASANIRKIQNCLDRILELEPDIKADFYFLFSIPFVQTWKKEFEE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHH
SLADDLNISKALAVVFESVKQINSLLDTNQSDSKQRIEYIQILAYFDRILGVLNFESKKD
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LLDSEIDSLIEERQIARKNKDFARSDAIRDQLLAQGILIEDTKEGIRWRKK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA