Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 45657634

GI number: 45657634

Start: 2160509

End: 2161585

Strand: Direct

Name: leuB

Synonym: LIC11768

Alternate gene names: 45657634

Gene position: 2160509-2161585 (Clockwise)

Preceding gene: 45657633

Following gene: 45657635

Centisome position: 50.51

GC content: 41.5

Gene sequence:

>1077_bases
ATGAAGAATGTAGCAGTACTTTCCGGAGACGGAATCGGACCGGAAGTCATGGAGATAGCCATCTCCGTTTTGAAAAAGGC
TCTCGGTGCAAAAGTTTCCGAGTTTCAATTTAAAGAAGGATTTGTAGGTGGAATCGCAATCGATAAAACCGGACACCCAC
TTCCACCGGAAACTCTTAAACTATGTGAAGAATCTTCCGCAATTCTTTTCGGAAGTGTGGGAGGTCCTAAATGGGAAACA
CTCCCTCCGGAAAAACAACCGGAACGAGGAGCACTTCTACCTTTGAGAAAACATTTTGATCTATTTGCAAATTTAAGACC
TGCGATCATTTATCCAGAGTTGAAAAATGCTTCTCCAGTTCGTTCTGATATTATTGGAAACGGATTAGATATTCTCATAT
TAAGAGAGTTAACCGGAGGAATTTATTTCGGACAACCAAAAGGAAGAGAAGGATCAGGTCAGGAAGAATTTGCATACGAC
ACGATGAAGTATTCCAGAAGAGAAATCGAAAGGATTGCTAAAGTCGCATTCCAGGCGGCCAGAAAAAGAAATAATAAAGT
GACTAGTATCGATAAAGCAAACGTCTTGACTACTTCCGTTTTTTGGAAGGAAGTAGTAATCGAATTGCATAAGAAAGAAT
TTTCAGACGTCCAATTGAATCATCTTTATGTGGACAATGCGGCGATGCAGTTAATCGTAAATCCGAAACAATTCGACGTG
GTTCTTTGTGAGAATATGTTTGGTGATATTCTTTCGGACGAGGCTTCCATCATTACTGGTTCAATCGGAATGCTTCCTTC
TGCCTCTCTTTCCGAATCTGGATTTGGATTGTATGAACCTTCTGGTGGTTCTGCGCCGGACATAGCCGGAAAAGGAGTGG
CAAATCCGATTGCTCAAGTATTGAGTGCGGCGTTGATGTTACGTTATTCTTTTTCTATGGAAGAAGAAGCAAACAAGATA
GAAACCGCCGTGCGTAAAACGATTGCCTCCGGAAAAAGAACCAGAGACATAGCGGAAGTAGGATCTACGATCGTAGGAAC
TAAAGAAATCGGTCAATTGATCGAATCCTTTCTCTAA

Upstream 100 bases:

>100_bases
CGGATTATGCCTCCACTTACAATTTCGACAGATTTTTTAAATCAAGGATTAGACATCTTAGAATCCGTACTCAAACAAAA
CTAAAAGGATCTAAAAAAGA

Downstream 100 bases:

>100_bases
AAAGAGGTAAAAAAATATGAAAGCAGGTGTAGCTCCCAACGGAAGACCGTATCAAGTATTGATCGCAGAAAATTCCAGAT
TCCAAGCAAAACAGTTGGCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKNVAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLKLCEESSAILFGSVGGPKWET
LPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPVRSDIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYD
TMKYSRREIERIAKVAFQAARKRNNKVTSIDKANVLTTSVFWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQVLSAALMLRYSFSMEEEANKI
ETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESFL

Sequences:

>Translated_358_residues
MKNVAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLKLCEESSAILFGSVGGPKWET
LPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPVRSDIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYD
TMKYSRREIERIAKVAFQAARKRNNKVTSIDKANVLTTSVFWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQVLSAALMLRYSFSMEEEANKI
ETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESFL
>Mature_358_residues
MKNVAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLKLCEESSAILFGSVGGPKWET
LPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPVRSDIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYD
TMKYSRREIERIAKVAFQAARKRNNKVTSIDKANVLTTSVFWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQVLSAALMLRYSFSMEEEANKI
ETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESFL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=368, Percent_Identity=32.0652173913043, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI28178821, Length=353, Percent_Identity=29.4617563739377, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI28178816, Length=332, Percent_Identity=29.8192771084337, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4758582, Length=343, Percent_Identity=27.1137026239067, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=34.3434343434343, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=27.2445820433437, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=61.2359550561798, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=33.2409972299169, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=24.0694789081886, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=352, Percent_Identity=28.6931818181818, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=367, Percent_Identity=28.8828337874659, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=29.1428571428571, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=339, Percent_Identity=28.9085545722714, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=43.4659090909091, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=353, Percent_Identity=33.1444759206799, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=278, Percent_Identity=34.5323741007194, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=30.8781869688385, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24643268, Length=338, Percent_Identity=32.5443786982249, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643270, Length=338, Percent_Identity=32.5443786982249, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=28.2798833819242, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078637, Length=361, Percent_Identity=27.9778393351801, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078635, Length=361, Percent_Identity=27.9778393351801, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078633, Length=361, Percent_Identity=27.9778393351801, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650122, Length=361, Percent_Identity=27.9778393351801, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=271, Percent_Identity=30.6273062730627, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362242, Length=356, Percent_Identity=26.685393258427, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648872, Length=356, Percent_Identity=26.685393258427, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=26.530612244898, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_LEPIC (Q72RH7)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001720.1
- ProteinModelPortal:   Q72RH7
- SMR:   Q72RH7
- GeneID:   2771817
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11768
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   LINT267671:LIC_11768-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38871; Mature: 38871

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 135-135 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLK
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCCCHHHHH
LCEESSAILFGSVGGPKWETLPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPV
HHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCH
RSDIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYDTMKYSRREIERIAKVAFQAA
HHHHHCCCCCEEEEEEHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRNNKVTSIDKANVLTTSVFWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCHHHE
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQV
EHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
LSAALMLRYSFSMEEEANKIETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESFL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHHHHHHHC
>Mature Secondary Structure
MKNVAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLK
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEECCCCCCCCHHHHH
LCEESSAILFGSVGGPKWETLPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPV
HHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCH
RSDIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYDTMKYSRREIERIAKVAFQAA
HHHHHCCCCCEEEEEEHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRNNKVTSIDKANVLTTSVFWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCHHHE
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQV
EHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
LSAALMLRYSFSMEEEANKIETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESFL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA