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The map label for this gene is prfH

Identifier: 45657623

GI number: 45657623

Start: 2150919

End: 2151386

Strand: Reverse

Name: prfH

Synonym: LIC11757

Alternate gene names: 45657623

Gene position: 2151386-2150919 (Counterclockwise)

Preceding gene: 45657624

Following gene: 45657622

Centisome position: 50.3

GC content: 33.33

Gene sequence:

>468_bases
ATGGCTTCTCGTTTTCCGGTTTCTGTAGAAAAAGAATCCAAACTTCTTGAACTCATGGAAATTCTTCAAATCAAAGAAAC
CGAATTAGAAGAAAGTTTTACAAGAAGTGGGGGTAAGGGCGGACAAAACGTAAATAAAGTTTCAACAGCGGTTCATCTCA
GACACAAACCAACCGGAATCGAAGTTAAATGTTCCCTTTATAGAACTCAAGGTTTAAATCGTTATAAAGCGAGAGCCATT
CTCTGTGAAAAAATTCAAGATTTCAATCATAAGAATTTGGGGATTATAAGTGAAGATCAAAAAAAATTGATTCGTAATAA
ACAAAAAGATTCTAAACGAAAGAAAGAAAAGTATTCTAGAAAAAATCAGAGTTTTTCTACTGTTTCTTTAGAAGAGGATA
AAAATTTTGAAGTCGAATGGAAAGAAGTCAAAAACGAAGAAGAAAGACGTTCGACGGAACCTGATTGA

Upstream 100 bases:

>100_bases
TATAGATTGTAAGATTACTTATGTTTGTCTGGATGAAAATGGAAAACCGTTTCCTTATTTTAAAGATCATTTTGAACTTC
AGGATTTGAAGTAATTTTTT

Downstream 100 bases:

>100_bases
TAAAGATTAGAATGAATAGAGTTGTTGGAAAATGAATCCTTCATTTGTTTCTGTTTTATGAAAACGGTCGATTAAAACAG
TTTTGTCAATTGCCACTATG

Product: peptide chain release factor 2-like protein

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MASRFPVSVEKESKLLELMEILQIKETELEESFTRSGGKGGQNVNKVSTAVHLRHKPTGIEVKCSLYRTQGLNRYKARAI
LCEKIQDFNHKNLGIISEDQKKLIRNKQKDSKRKKEKYSRKNQSFSTVSLEEDKNFEVEWKEVKNEEERRSTEPD

Sequences:

>Translated_155_residues
MASRFPVSVEKESKLLELMEILQIKETELEESFTRSGGKGGQNVNKVSTAVHLRHKPTGIEVKCSLYRTQGLNRYKARAI
LCEKIQDFNHKNLGIISEDQKKLIRNKQKDSKRKKEKYSRKNQSFSTVSLEEDKNFEVEWKEVKNEEERRSTEPD
>Mature_154_residues
ASRFPVSVEKESKLLELMEILQIKETELEESFTRSGGKGGQNVNKVSTAVHLRHKPTGIEVKCSLYRTQGLNRYKARAIL
CEKIQDFNHKNLGIISEDQKKLIRNKQKDSKRKKEKYSRKNQSFSTVSLEEDKNFEVEWKEVKNEEERRSTEPD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Drosophila melanogaster, GI24654113, Length=83, Percent_Identity=43.3734939759036, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 18116; Mature: 17985

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRFPVSVEKESKLLELMEILQIKETELEESFTRSGGKGGQNVNKVSTAVHLRHKPTGI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCE
EVKCSLYRTQGLNRYKARAILCEKIQDFNHKNLGIISEDQKKLIRNKQKDSKRKKEKYSR
EEEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNQSFSTVSLEEDKNFEVEWKEVKNEEERRSTEPD
CCCCCEEEEECCCCCCCCHHHHHCCHHHHCCCCCC
>Mature Secondary Structure 
ASRFPVSVEKESKLLELMEILQIKETELEESFTRSGGKGGQNVNKVSTAVHLRHKPTGI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEECCCCE
EVKCSLYRTQGLNRYKARAILCEKIQDFNHKNLGIISEDQKKLIRNKQKDSKRKKEKYSR
EEEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNQSFSTVSLEEDKNFEVEWKEVKNEEERRSTEPD
CCCCCEEEEECCCCCCCCHHHHHCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA