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The map label for this gene is ptsI

Identifier: 45657504

GI number: 45657504

Start: 1998110

End: 1999939

Strand: Reverse

Name: ptsI

Synonym: LIC11632

Alternate gene names: 45657504

Gene position: 1999939-1998110 (Counterclockwise)

Preceding gene: 45657505

Following gene: 45657500

Centisome position: 46.76

GC content: 36.56

Gene sequence:

>1830_bases
TTGATTCGTTCTATTTTTGACTCAGAAAAGAATTGTCAGATTCTTCTTTTGGTGAATTCTGTAGTCGAGTTTGAAACTCG
TTTTAGAATGATTCCTTCCAAACGTACAGCTTTTCCTGGAATTACAGCCTTTCCGGGAAAACTATACGGTAAGGTCTTAA
AAACCGGAAAGAGAAGGAATACCATTCTCACAGGAACCTACGTTCACGAATCCGAAAAAGAAGCTGAAGTAGAAAAGTTC
TCTGTCGCTTTAGAAGAAAGTCTTCACAGCTTAAGAATTTTGATTACGTCTCTGGAAACCTCTGGACCAGATCAAAAAGA
AATCCAAGAGATTTTAGAAACACAGGCTATGATTTGTTCGGATCCTAGCCTTGCCACCTCGGTTCGAAAAAGAATTCTAG
AACTTGGAGAAAATGCAATCCTAGCGGTTCAAAACGTTACCCATGAAATTTCTGAAAAATTCAAAGCCATGGAAAATGAA
TTTTTCCGAGAAAGGGTAGATCATTTTCAAGATGTTTCCAATCGACTGATTGAATTTATCGCAGGTAAAAAAGAAGAAGA
TTCTTTTTTATCCGGTTTAAAAGAAGATTTAATCTTAGTCGCAAGAGAACTTACACCTTCACAGATGATTCTCATGGATA
AAACTCGTATCCGTGGAATCGCAACTGATCTAGGCGGAAAAACCGGACACATGGCTATTTTAGCCAGAAACTACGGAATC
CCTACAATCGTAGGATTGAAAGATTTTTCCTCTCATGTCAAAGACAACGAATTCATCTTTTTAGACGCAGAATCCGGAAA
CGCGATTCGTTTTCCCACTCTTGAAGAAGTAAAATACTACGGATTCTCTTCTTCCTACCCCACAGAAGAAAATGAAACCA
AAAAGATAAGGGCGATAACTAAGGACGGAATCAGAGTTAGGATCAAATGTAATTTAGAAACAGAATTAGATTGTGAACAA
GCGATCAAGTCCGGAGCGGAAGGGGTAGGACTTTTTAGAAGTGAGTCTTTATTTTTAAAATATCAGGATAGTAACGTTTC
TGGAGAAGAACAGTTCCTTGCTTATAAAGCAATTGCAGAAGGAATGCAGGACAAACCAGTAATTATTCGTACCTTTGACA
TTGGAGCCGATAAATTTTCAACAGGAGAATTAGAAGAAAATCCTTTTTTAGGAAATCGAGGGATAAGATATTCTTTATCC
AATCCAGAATGGTATTCTGAACAACTAACGGCAATTTTGCGAGCATCTGCTTTTGGAAATGTAAGTATATTACTACCTAT
GATCACTGGTCCCGTAGAAATTACTCGCACACGAAAACTTTTAGAAGAATGTAAACGCAAATTGAGCGCAAAAAAAGAAA
GATATAATAAAAAAATTAAAATCGGTGCCATGATTGAAACCCCTGCTGCAGTTGCGGCCTTAGATCTAATCGCAAGAGAA
GCGGATTTTTTTTCTATAGGAACAAACGATCTACTTCAATATATCATGGCAGTAGATAGAAACAATATTCACGTTTCTTC
TTTATATAATCCATATCATATTTCTTTTTTAAGAGCTTTGATTCGAATCGTGGAAGTTTCTAGGGATTACGACAAACCCC
TAAGTCTTTGTGGAGAACTTGCATCAGATACTGATTTTACCATTTTTTTAGTTGGTATTGGCATCCGTGAACTTTCGGTT
TCAATTCCTTTTTTAAGTCCAATTCGAAAAATCATTCGATCGATTTCGCTGCATCAAGCAGGAATCTTGGTAAGAAAAAT
TCTAGAACTTTCCGAACAAGAAAATTATGAAAGTATTGAAGCATTCCTTTTTAGCAAACATCTAAGCTAA

Upstream 100 bases:

>100_bases
TAAATATAAAAGTCGTAGAATCCCACTATTCAAAAGAAAAGCAGTCGTCGTTTAAACGTTTTCATTTAGTAATTTCAATT
TCGGTCAAATCTGAGTCTTT

Downstream 100 bases:

>100_bases
AACTTTTCGATTTCTAATATTAAAAACACAAAGGAATCCCAAATTCTTTCCTTTCCTTGAAAAAAAACAGGTCCCAAACT
TTCTTTTTCTTTTTTTAAAC

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MIRSIFDSEKNCQILLLVNSVVEFETRFRMIPSKRTAFPGITAFPGKLYGKVLKTGKRRNTILTGTYVHESEKEAEVEKF
SVALEESLHSLRILITSLETSGPDQKEIQEILETQAMICSDPSLATSVRKRILELGENAILAVQNVTHEISEKFKAMENE
FFRERVDHFQDVSNRLIEFIAGKKEEDSFLSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGI
PTIVGLKDFSSHVKDNEFIFLDAESGNAIRFPTLEEVKYYGFSSSYPTEENETKKIRAITKDGIRVRIKCNLETELDCEQ
AIKSGAEGVGLFRSESLFLKYQDSNVSGEEQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLS
NPEWYSEQLTAILRASAFGNVSILLPMITGPVEITRTRKLLEECKRKLSAKKERYNKKIKIGAMIETPAAVAALDLIARE
ADFFSIGTNDLLQYIMAVDRNNIHVSSLYNPYHISFLRALIRIVEVSRDYDKPLSLCGELASDTDFTIFLVGIGIRELSV
SIPFLSPIRKIIRSISLHQAGILVRKILELSEQENYESIEAFLFSKHLS

Sequences:

>Translated_609_residues
MIRSIFDSEKNCQILLLVNSVVEFETRFRMIPSKRTAFPGITAFPGKLYGKVLKTGKRRNTILTGTYVHESEKEAEVEKF
SVALEESLHSLRILITSLETSGPDQKEIQEILETQAMICSDPSLATSVRKRILELGENAILAVQNVTHEISEKFKAMENE
FFRERVDHFQDVSNRLIEFIAGKKEEDSFLSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGI
PTIVGLKDFSSHVKDNEFIFLDAESGNAIRFPTLEEVKYYGFSSSYPTEENETKKIRAITKDGIRVRIKCNLETELDCEQ
AIKSGAEGVGLFRSESLFLKYQDSNVSGEEQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLS
NPEWYSEQLTAILRASAFGNVSILLPMITGPVEITRTRKLLEECKRKLSAKKERYNKKIKIGAMIETPAAVAALDLIARE
ADFFSIGTNDLLQYIMAVDRNNIHVSSLYNPYHISFLRALIRIVEVSRDYDKPLSLCGELASDTDFTIFLVGIGIRELSV
SIPFLSPIRKIIRSISLHQAGILVRKILELSEQENYESIEAFLFSKHLS
>Mature_609_residues
MIRSIFDSEKNCQILLLVNSVVEFETRFRMIPSKRTAFPGITAFPGKLYGKVLKTGKRRNTILTGTYVHESEKEAEVEKF
SVALEESLHSLRILITSLETSGPDQKEIQEILETQAMICSDPSLATSVRKRILELGENAILAVQNVTHEISEKFKAMENE
FFRERVDHFQDVSNRLIEFIAGKKEEDSFLSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGI
PTIVGLKDFSSHVKDNEFIFLDAESGNAIRFPTLEEVKYYGFSSSYPTEENETKKIRAITKDGIRVRIKCNLETELDCEQ
AIKSGAEGVGLFRSESLFLKYQDSNVSGEEQFLAYKAIAEGMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLS
NPEWYSEQLTAILRASAFGNVSILLPMITGPVEITRTRKLLEECKRKLSAKKERYNKKIKIGAMIETPAAVAALDLIARE
ADFFSIGTNDLLQYIMAVDRNNIHVSSLYNPYHISFLRALIRIVEVSRDYDKPLSLCGELASDTDFTIFLVGIGIRELSV
SIPFLSPIRKIIRSISLHQAGILVRKILELSEQENYESIEAFLFSKHLS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=556, Percent_Identity=36.6906474820144, Blast_Score=321, Evalue=9e-89,
Organism=Escherichia coli, GI1788726, Length=566, Percent_Identity=31.4487632508834, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789193, Length=585, Percent_Identity=31.1111111111111, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI48994992, Length=463, Percent_Identity=34.341252699784, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1787994, Length=408, Percent_Identity=25.7352941176471, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI226510935, Length=217, Percent_Identity=24.4239631336406, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 68705; Mature: 68705

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSIFDSEKNCQILLLVNSVVEFETRFRMIPSKRTAFPGITAFPGKLYGKVLKTGKRRN
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
TILTGTYVHESEKEAEVEKFSVALEESLHSLRILITSLETSGPDQKEIQEILETQAMICS
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCHHHHHHHHHHHHHHCC
DPSLATSVRKRILELGENAILAVQNVTHEISEKFKAMENEFFRERVDHFQDVSNRLIEFI
CCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGKKEEDSFLSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHEHHHHCCCCCCCEEEEEECCCC
PTIVGLKDFSSHVKDNEFIFLDAESGNAIRFPTLEEVKYYGFSSSYPTEENETKKIRAIT
CEEEEHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCCCCCHHHHEEE
KDGIRVRIKCNLETELDCEQAIKSGAEGVGLFRSESLFLKYQDSNVSGEEQFLAYKAIAE
CCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHC
GMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLSNPEWYSEQLTAILRASAFGN
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCC
VSILLPMITGPVEITRTRKLLEECKRKLSAKKERYNKKIKIGAMIETPAAVAALDLIARE
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHC
ADFFSIGTNDLLQYIMAVDRNNIHVSSLYNPYHISFLRALIRIVEVSRDYDKPLSLCGEL
CCEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ASDTDFTIFLVGIGIRELSVSIPFLSPIRKIIRSISLHQAGILVRKILELSEQENYESIE
CCCCCEEEEEEEECHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AFLFSKHLS
HHHHHHCCC
>Mature Secondary Structure
MIRSIFDSEKNCQILLLVNSVVEFETRFRMIPSKRTAFPGITAFPGKLYGKVLKTGKRRN
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
TILTGTYVHESEKEAEVEKFSVALEESLHSLRILITSLETSGPDQKEIQEILETQAMICS
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCCHHHHHHHHHHHHHHCC
DPSLATSVRKRILELGENAILAVQNVTHEISEKFKAMENEFFRERVDHFQDVSNRLIEFI
CCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGKKEEDSFLSGLKEDLILVARELTPSQMILMDKTRIRGIATDLGGKTGHMAILARNYGI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHEHHHHCCCCCCCEEEEEECCCC
PTIVGLKDFSSHVKDNEFIFLDAESGNAIRFPTLEEVKYYGFSSSYPTEENETKKIRAIT
CEEEEHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCCCCCHHHHEEE
KDGIRVRIKCNLETELDCEQAIKSGAEGVGLFRSESLFLKYQDSNVSGEEQFLAYKAIAE
CCCEEEEEEECCCCCCCHHHHHHCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHC
GMQDKPVIIRTFDIGADKFSTGELEENPFLGNRGIRYSLSNPEWYSEQLTAILRASAFGN
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCC
VSILLPMITGPVEITRTRKLLEECKRKLSAKKERYNKKIKIGAMIETPAAVAALDLIARE
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHC
ADFFSIGTNDLLQYIMAVDRNNIHVSSLYNPYHISFLRALIRIVEVSRDYDKPLSLCGEL
CCEEECCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ASDTDFTIFLVGIGIRELSVSIPFLSPIRKIIRSISLHQAGILVRKILELSEQENYESIE
CCCCCEEEEEEEECHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AFLFSKHLS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]