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The map label for this gene is sbp

Identifier: 45657150

GI number: 45657150

Start: 1565101

End: 1566105

Strand: Reverse

Name: sbp

Synonym: LIC11267

Alternate gene names: 45657150

Gene position: 1566105-1565101 (Counterclockwise)

Preceding gene: 45657151

Following gene: 45657148

Centisome position: 36.62

GC content: 37.91

Gene sequence:

>1005_bases
ATGAAAATAAAATTTTTTAAAATTACCACCATCGTGTTTTTTACAATGACCTCGAACCTAGGGCTCTTTGCAAAAGATAT
AACTCTACTTAACGTATCTTATGATCCAACAAGAGAACTATACGCCGAATACAACAAACTTTTTGCAAGTTATTGGAAAT
CTAAAACTGGAGACGAAGTAAGAGTCAATCAATCCCACGGAGGAAGTGGAAAACAGGCACGATCTGTGATCGACGGTTTA
GAAGCTGATATTGTTACTCTTGCACTCGCATACGATATAGATATCATCGCGTCCAAAGGTTTAATTTCTAGGGATTGGCT
AAAAAGTCTTCCAAACAACAGTACTCCTTATACTTCTACCATCGTGTTTGTAGTTAGAAAAGGTAATCCTAAAAATATAA
AAGACTGGGACGACCTAGTTAAATCAAAAGTAAGTGTAATTACTCCAAATCCTAAAACAAGTGGAGGAGCCAGATGGAAC
TATCTCGCTGCTTGGGCCTTTGCACAAAAAAAGTTCAACAACGATACAAATAAAGTTCAAGACTTTATAAAAACATTATA
TAAGAATGTTCCAGTTTTAGATTCAGGGGCTAGAGGTTCTAGCAATACTTTTGCACAAAACGGAATCGGAGACGTCTTGA
TCGCTTGGGAAAACGAATCCTTCCTCATTCTAGAAGAATTTGGTAAGGACAAATATGAAGTAGTCATTCCATCTTTGAGC
ATTCTCGCCGAACCACCCGTAGCAATCGTGGAAAAAAATGCGGATAAACACGGAAATTTAGAAACTGCAAAAGCCTATCT
CAAATCCCTCTACTCCGAGGCGGCGCAAGAAATTATTGCTAAACATGGTTATCGCCCTAGAAGCCAAACCATACTTAAAA
AATATGAGACTAAATTCCCAAAACTAGATCTAATCACAGTAGACGGACATTTCAACGGTTGGCAAAAAGCACATAAAGAC
CATTTTGCTGACGGCGCTTCTTTCGATCAGCTCTATGTCAAATAA

Upstream 100 bases:

>100_bases
AACATAAACGACAGATATACACTGGACAATAGAGCCTATAGTTTTTTTCTGTTCGTTCAATTTGCAATGTAATTCATAAA
ATTGGAATTAGGAAATCAGC

Downstream 100 bases:

>100_bases
AATAGATATACTCTTTTTAAATGAAAAATAAAACTATATCTTTTTTCCATTATTCAATGTCATATAATAGGTTATATAAA
TTTCTCTTAGAAAAGCGTAC

Product: periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKIKFFKITTIVFFTMTSNLGLFAKDITLLNVSYDPTRELYAEYNKLFASYWKSKTGDEVRVNQSHGGSGKQARSVIDGL
EADIVTLALAYDIDIIASKGLISRDWLKSLPNNSTPYTSTIVFVVRKGNPKNIKDWDDLVKSKVSVITPNPKTSGGARWN
YLAAWAFAQKKFNNDTNKVQDFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVVIPSLS
ILAEPPVAIVEKNADKHGNLETAKAYLKSLYSEAAQEIIAKHGYRPRSQTILKKYETKFPKLDLITVDGHFNGWQKAHKD
HFADGASFDQLYVK

Sequences:

>Translated_334_residues
MKIKFFKITTIVFFTMTSNLGLFAKDITLLNVSYDPTRELYAEYNKLFASYWKSKTGDEVRVNQSHGGSGKQARSVIDGL
EADIVTLALAYDIDIIASKGLISRDWLKSLPNNSTPYTSTIVFVVRKGNPKNIKDWDDLVKSKVSVITPNPKTSGGARWN
YLAAWAFAQKKFNNDTNKVQDFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVVIPSLS
ILAEPPVAIVEKNADKHGNLETAKAYLKSLYSEAAQEIIAKHGYRPRSQTILKKYETKFPKLDLITVDGHFNGWQKAHKD
HFADGASFDQLYVK
>Mature_334_residues
MKIKFFKITTIVFFTMTSNLGLFAKDITLLNVSYDPTRELYAEYNKLFASYWKSKTGDEVRVNQSHGGSGKQARSVIDGL
EADIVTLALAYDIDIIASKGLISRDWLKSLPNNSTPYTSTIVFVVRKGNPKNIKDWDDLVKSKVSVITPNPKTSGGARWN
YLAAWAFAQKKFNNDTNKVQDFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVVIPSLS
ILAEPPVAIVEKNADKHGNLETAKAYLKSLYSEAAQEIIAKHGYRPRSQTILKKYETKFPKLDLITVDGHFNGWQKAHKD
HFADGASFDQLYVK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=310, Percent_Identity=66.1290322580645, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=48.1967213114754, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37479; Mature: 37479

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKFFKITTIVFFTMTSNLGLFAKDITLLNVSYDPTRELYAEYNKLFASYWKSKTGDEV
CEEEEEEEEEEEEEEHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
RVNQSHGGSGKQARSVIDGLEADIVTLALAYDIDIIASKGLISRDWLKSLPNNSTPYTST
EEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCCEEE
IVFVVRKGNPKNIKDWDDLVKSKVSVITPNPKTSGGARWNYLAAWAFAQKKFNNDTNKVQ
EEEEEECCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
DFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVVIPSLS
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEHHCCCCCEEEEECCHH
ILAEPPVAIVEKNADKHGNLETAKAYLKSLYSEAAQEIIAKHGYRPRSQTILKKYETKFP
HHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
KLDLITVDGHFNGWQKAHKDHFADGASFDQLYVK
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEC
>Mature Secondary Structure
MKIKFFKITTIVFFTMTSNLGLFAKDITLLNVSYDPTRELYAEYNKLFASYWKSKTGDEV
CEEEEEEEEEEEEEEHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEE
RVNQSHGGSGKQARSVIDGLEADIVTLALAYDIDIIASKGLISRDWLKSLPNNSTPYTST
EEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCCEEE
IVFVVRKGNPKNIKDWDDLVKSKVSVITPNPKTSGGARWNYLAAWAFAQKKFNNDTNKVQ
EEEEEECCCCCCCCHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
DFIKTLYKNVPVLDSGARGSSNTFAQNGIGDVLIAWENESFLILEEFGKDKYEVVIPSLS
HHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEHHCCCCCEEEEECCHH
ILAEPPVAIVEKNADKHGNLETAKAYLKSLYSEAAQEIIAKHGYRPRSQTILKKYETKFP
HHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
KLDLITVDGHFNGWQKAHKDHFADGASFDQLYVK
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]