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The map label for this gene is glpF

Identifier: 45656620

GI number: 45656620

Start: 879309

End: 880025

Strand: Reverse

Name: glpF

Synonym: LIC10722

Alternate gene names: 45656620

Gene position: 880025-879309 (Counterclockwise)

Preceding gene: 45656624

Following gene: 45656619

Centisome position: 20.57

GC content: 41.98

Gene sequence:

>717_bases
ATGGGATCGCCTATTTTAGGAGAATTTTTAGGAACTTTTGTATTGATTCTTTTGGGAAACGGAGTTGTAGCCGGGGTTTT
GCTTGAAAAAAGTAAATCCAAAGATGGAGGCTGGATCGTAATTACGGCAGGCTGGGCCTTTGCGGTGATGATCGGAGTTT
TTGTTTCCAACGCATTTGGAAGTTTAGACGCACATCTCAATCCCGCCGTGACGCTTGCGTTTGCAATCCAATCCGGAGAT
TACACTAAATTGATATTTTATATTCCCGCACAAATTGCAGGAGCGTTTTTAGGAGCCGTTTTCAATTACCTGCACTATCT
ACCGCATTGGAAAGAAACCAAAGATCCGGGAAAAATTCTAGCCGTGTTCTCCACCGAACCAGCAATTTCGCATAACGTTT
ATAATTTTTTCAGCGAGTTTTTAGGAACCTTTTTACTCATCTTGGGAATCGTTTCTATTTTCTCACCCAAACTACAAGGT
TTAAGCGTTCATTTTGGGACTTTTTTAGTAGGAATTCTTGTTTGGAGTATCGGTCTTTCCATGGGAGGAACGACCGGTTA
TGCAATCAATCCGGCCAGAGATTTAGGACCAAGAATTGCCCATTTTATTTTACCCATAGCAGGAAAAGGAACCTCCAATT
GGAAATACGCTTGGTTGCCTGTATTCGCTCCTTTAAGCGGGGCTGCCTGCGCGGGAATATTAATTCGATTTTTATAA

Upstream 100 bases:

>100_bases
ATCTTTTAAACAGCCATTTTTAGGTATTTTTGTTGAGTAAAAATAGAATTTCAGAACCTTGTCTGGGTTTTAGTTTCGAT
CATTAGAGTTAGGGGGAAGG

Downstream 100 bases:

>100_bases
TATATTAAAGAATAATATTTTAATGTTATATAGAGTCCTCCAAACAATTTTTTGAAAAAGAATATAACTTTTTCAGAATC
ATTGGAGTTTTTTAAAATTA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MGSPILGEFLGTFVLILLGNGVVAGVLLEKSKSKDGGWIVITAGWAFAVMIGVFVSNAFGSLDAHLNPAVTLAFAIQSGD
YTKLIFYIPAQIAGAFLGAVFNYLHYLPHWKETKDPGKILAVFSTEPAISHNVYNFFSEFLGTFLLILGIVSIFSPKLQG
LSVHFGTFLVGILVWSIGLSMGGTTGYAINPARDLGPRIAHFILPIAGKGTSNWKYAWLPVFAPLSGAACAGILIRFL

Sequences:

>Translated_238_residues
MGSPILGEFLGTFVLILLGNGVVAGVLLEKSKSKDGGWIVITAGWAFAVMIGVFVSNAFGSLDAHLNPAVTLAFAIQSGD
YTKLIFYIPAQIAGAFLGAVFNYLHYLPHWKETKDPGKILAVFSTEPAISHNVYNFFSEFLGTFLLILGIVSIFSPKLQG
LSVHFGTFLVGILVWSIGLSMGGTTGYAINPARDLGPRIAHFILPIAGKGTSNWKYAWLPVFAPLSGAACAGILIRFL
>Mature_237_residues
GSPILGEFLGTFVLILLGNGVVAGVLLEKSKSKDGGWIVITAGWAFAVMIGVFVSNAFGSLDAHLNPAVTLAFAIQSGDY
TKLIFYIPAQIAGAFLGAVFNYLHYLPHWKETKDPGKILAVFSTEPAISHNVYNFFSEFLGTFLLILGIVSIFSPKLQGL
SVHFGTFLVGILVWSIGLSMGGTTGYAINPARDLGPRIAHFILPIAGKGTSNWKYAWLPVFAPLSGAACAGILIRFL

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=236, Percent_Identity=36.864406779661, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI22538420, Length=242, Percent_Identity=34.297520661157, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=34.2741935483871, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI4502187, Length=248, Percent_Identity=34.2741935483871, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1790362, Length=238, Percent_Identity=31.0924369747899, Blast_Score=85, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994009, Length=232, Percent_Identity=34.4827586206897, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=247, Percent_Identity=29.9595141700405, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17531431, Length=244, Percent_Identity=31.1475409836066, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17531429, Length=244, Percent_Identity=31.1475409836066, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=29.7959183673469, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32564052, Length=245, Percent_Identity=29.7959183673469, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71992966, Length=238, Percent_Identity=30.672268907563, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=204, Percent_Identity=34.3137254901961, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322985, Length=273, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25410; Mature: 25279

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPILGEFLGTFVLILLGNGVVAGVLLEKSKSKDGGWIVITAGWAFAVMIGVFVSNAFG
CCCCHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHC
SLDAHLNPAVTLAFAIQSGDYTKLIFYIPAQIAGAFLGAVFNYLHYLPHWKETKDPGKIL
CCCCCCCCEEEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AVFSTEPAISHNVYNFFSEFLGTFLLILGIVSIFSPKLQGLSVHFGTFLVGILVWSIGLS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHC
MGGTTGYAINPARDLGPRIAHFILPIAGKGTSNWKYAWLPVFAPLSGAACAGILIRFL
CCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
GSPILGEFLGTFVLILLGNGVVAGVLLEKSKSKDGGWIVITAGWAFAVMIGVFVSNAFG
CCCHHHHHHHHHHHHHHCCCHHHHHEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHC
SLDAHLNPAVTLAFAIQSGDYTKLIFYIPAQIAGAFLGAVFNYLHYLPHWKETKDPGKIL
CCCCCCCCEEEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
AVFSTEPAISHNVYNFFSEFLGTFLLILGIVSIFSPKLQGLSVHFGTFLVGILVWSIGLS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHC
MGGTTGYAINPARDLGPRIAHFILPIAGKGTSNWKYAWLPVFAPLSGAACAGILIRFL
CCCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]