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The map label for this gene is bedB

Identifier: 45656531

GI number: 45656531

Start: 772522

End: 772833

Strand: Direct

Name: bedB

Synonym: LIC10633

Alternate gene names: 45656531

Gene position: 772522-772833 (Clockwise)

Preceding gene: 45656530

Following gene: 45656532

Centisome position: 18.06

GC content: 43.27

Gene sequence:

>312_bases
ATGAGCTTTCGTAAACTCGCAAAACTGTCCGAAATAGAAAACGGGAAAGTCAAAGTAATTGAAACCAGATATAATAGAAT
TGGGATTACAAGTTTAGACGGAAATGTTTACGCGTTTGAAGATGTATGCACACATGACGGAGAAGCCATTTCGGAAGGAG
AATTGTGTGGGGACGTAATTACTTGTCCGAGACACGAGGCGCAGTTTTCCATCAAAACCGGAAAAGCGCTTTGTATGCCC
GCTGTGGAAAATCTTCCGGTTTATCCGGTGAGAATCGTAGATGACTCCGTCGAGGTAGATTTGGAGGATTGA

Upstream 100 bases:

>100_bases
TTGTGGACGGATTTTTGGGCCAAGTGATTGGTGAAATCGAATCGAATTCCATACAAGAAGAGCTATTTCGGTTAATTGCT
AGAAAAGTGGAGGGGGCTAT

Downstream 100 bases:

>100_bases
CAGATATGAGTTTCTCTGCCGAAAGAATTAGAACCGACTTCCCGATTTTGGGAACTAAGATGAACGGGAAACCACTCGTT
TTTTTAGATAGCGCCGCTAG

Product: benzene 1,2-dioxygenase system ferredoxin component

Products: cis-cyclohexa-3,5-diene-1,2-diol; NAD+

Alternate protein names: NA

Number of amino acids: Translated: 103; Mature: 102

Protein sequence:

>103_residues
MSFRKLAKLSEIENGKVKVIETRYNRIGITSLDGNVYAFEDVCTHDGEAISEGELCGDVITCPRHEAQFSIKTGKALCMP
AVENLPVYPVRIVDDSVEVDLED

Sequences:

>Translated_103_residues
MSFRKLAKLSEIENGKVKVIETRYNRIGITSLDGNVYAFEDVCTHDGEAISEGELCGDVITCPRHEAQFSIKTGKALCMP
AVENLPVYPVRIVDDSVEVDLED
>Mature_102_residues
SFRKLAKLSEIENGKVKVIETRYNRIGITSLDGNVYAFEDVCTHDGEAISEGELCGDVITCPRHEAQFSIKTGKALCMPA
VENLPVYPVRIVDDSVEVDLED

Specific function: This protein seems to be a 2Fe-2S ferredoxin [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788890, Length=97, Percent_Identity=36.0824742268041, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.12.3

Molecular weight: Translated: 11404; Mature: 11273

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRKLAKLSEIENGKVKVIETRYNRIGITSLDGNVYAFEDVCTHDGEAISEGELCGDVI
CCHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEE
TCPRHEAQFSIKTGKALCMPAVENLPVYPVRIVDDSVEVDLED
ECCCCCCEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEECC
>Mature Secondary Structure 
SFRKLAKLSEIENGKVKVIETRYNRIGITSLDGNVYAFEDVCTHDGEAISEGELCGDVI
CHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEE
TCPRHEAQFSIKTGKALCMPAVENLPVYPVRIVDDSVEVDLED
ECCCCCCEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: benzene; NADH; H+; O2

Specific reaction: benzene + NADH + H+ + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344526 [H]