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The map label for this gene is kdsB

Identifier: 45656523

GI number: 45656523

Start: 762578

End: 763324

Strand: Direct

Name: kdsB

Synonym: LIC10625

Alternate gene names: 45656523

Gene position: 762578-763324 (Clockwise)

Preceding gene: 45656522

Following gene: 45656529

Centisome position: 17.83

GC content: 38.42

Gene sequence:

>747_bases
TTGATGAAAAAAATTTTAGGAGTCATTCCTGCGCGTTATGCAAGTTCTCGTTTTCCGGGAAAACCTCTCGCAAAAATAGG
GGATAAGACCATGATAGAATGGACTTATCGGAATGCTTCTAGGTCCTCTGTCTTATCTGAGTTAGTTGTTGCGACTGATG
ATGTTAGGATTCATGAAGTTGTTCAAAAATTTGGTGGTAGAAGTGTGATGACTAGTTCGGATCATCCTTCCGGAACTGAT
CGAATTATTGAAGTTGCTAATCAATTTTCTGAATATTCGATAATTGTAAATATACAAGGAGACGAACCAGGAATTGAACC
GGAATTAATTGACGGAGTTGCAAGTTTAAAAGCCTCTCATCCGGAATGGGCGATGAGTACAGCTGCAGTTCCTTTATTAG
ATTTTTCTCATGCAATCGATTTCAACCGGGTCAAGGTAATTATAGATCGAAACGGAAAGGCAATCTATTTTTCCAGATCT
TTGATTCCGAGTCAATTTAAGACTACGGTTCCTTTGTATCGTCATTTGGGAATTTATGGATACGATCGGGATTTCCTTTT
GCAATACAATTCTCTTCCCAAAAGTAATTTAGAAGAATCGGAATCTTTGGAACAACTCAGAGCGATCGAAGCGGGTTATG
GAATTGGAATTTATTTGTCCAAGGAAGCTGGATTGTCCGTAGATACACCCGCCGATTTGGAGATCGTGATTGAAGATTTT
AAAAAACGAAAATGGATTTCTGAATAA

Upstream 100 bases:

>100_bases
AAGATCAATTGGATCTCAGAGAAGAAATTAAAGCGCAAGTAAATCATATTCTTACTGAAGGAAAGATCCAGGAAGTTTAT
TTTACTGAATTTATAGTCAA

Downstream 100 bases:

>100_bases
AAAGAGAATCAAGAGAACTCATTAACTCATCTAATCGAAACTATAACGGAGTTTCTCATTTTATTTTTACAAAAAAATCA
GGCTTTATAAAATGAATCTC

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MMKKILGVIPARYASSRFPGKPLAKIGDKTMIEWTYRNASRSSVLSELVVATDDVRIHEVVQKFGGRSVMTSSDHPSGTD
RIIEVANQFSEYSIIVNIQGDEPGIEPELIDGVASLKASHPEWAMSTAAVPLLDFSHAIDFNRVKVIIDRNGKAIYFSRS
LIPSQFKTTVPLYRHLGIYGYDRDFLLQYNSLPKSNLEESESLEQLRAIEAGYGIGIYLSKEAGLSVDTPADLEIVIEDF
KKRKWISE

Sequences:

>Translated_248_residues
MMKKILGVIPARYASSRFPGKPLAKIGDKTMIEWTYRNASRSSVLSELVVATDDVRIHEVVQKFGGRSVMTSSDHPSGTD
RIIEVANQFSEYSIIVNIQGDEPGIEPELIDGVASLKASHPEWAMSTAAVPLLDFSHAIDFNRVKVIIDRNGKAIYFSRS
LIPSQFKTTVPLYRHLGIYGYDRDFLLQYNSLPKSNLEESESLEQLRAIEAGYGIGIYLSKEAGLSVDTPADLEIVIEDF
KKRKWISE
>Mature_248_residues
MMKKILGVIPARYASSRFPGKPLAKIGDKTMIEWTYRNASRSSVLSELVVATDDVRIHEVVQKFGGRSVMTSSDHPSGTD
RIIEVANQFSEYSIIVNIQGDEPGIEPELIDGVASLKASHPEWAMSTAAVPLLDFSHAIDFNRVKVIIDRNGKAIYFSRS
LIPSQFKTTVPLYRHLGIYGYDRDFLLQYNSLPKSNLEESESLEQLRAIEAGYGIGIYLSKEAGLSVDTPADLEIVIEDF
KKRKWISE

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=245, Percent_Identity=42.0408163265306, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB_LEPIC (Q72UN2)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000609.1
- HSSP:   P44490
- ProteinModelPortal:   Q72UN2
- SMR:   Q72UN2
- GeneID:   2769815
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10625
- HOGENOM:   HBG637773
- OMA:   IIPARLK
- ProtClustDB:   PRK05450
- BioCyc:   LINT267671:LIC_10625-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 27727; Mature: 27727

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKILGVIPARYASSRFPGKPLAKIGDKTMIEWTYRNASRSSVLSELVVATDDVRIHEV
CHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHH
VQKFGGRSVMTSSDHPSGTDRIIEVANQFSEYSIIVNIQGDEPGIEPELIDGVASLKASH
HHHHCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHCCC
PEWAMSTAAVPLLDFSHAIDFNRVKVIIDRNGKAIYFSRSLIPSQFKTTVPLYRHLGIYG
CCHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
YDRDFLLQYNSLPKSNLEESESLEQLRAIEAGYGIGIYLSKEAGLSVDTPADLEIVIEDF
CCHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
KKRKWISE
HHHCCCCC
>Mature Secondary Structure
MMKKILGVIPARYASSRFPGKPLAKIGDKTMIEWTYRNASRSSVLSELVVATDDVRIHEV
CHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHH
VQKFGGRSVMTSSDHPSGTDRIIEVANQFSEYSIIVNIQGDEPGIEPELIDGVASLKASH
HHHHCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHCCC
PEWAMSTAAVPLLDFSHAIDFNRVKVIIDRNGKAIYFSRSLIPSQFKTTVPLYRHLGIYG
CCHHHHHHCCHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
YDRDFLLQYNSLPKSNLEESESLEQLRAIEAGYGIGIYLSKEAGLSVDTPADLEIVIEDF
CCHHHEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
KKRKWISE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA