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The map label for this gene is pldB

Identifier: 45656500

GI number: 45656500

Start: 734918

End: 735847

Strand: Reverse

Name: pldB

Synonym: LIC10602

Alternate gene names: 45656500

Gene position: 735847-734918 (Counterclockwise)

Preceding gene: 45656504

Following gene: 45656499

Centisome position: 17.2

GC content: 38.82

Gene sequence:

>930_bases
ATGAAATTTTTTTCTCTTTGCAAAGGATCATTTGCATTTATAACACGTTTCGCTATGTCATGGATAAATTCTTATAATTT
AAAAGACGATACCTTTGCGGGAAGCGGGGGATCTAAAATTTTCTATCGAACCTACCAACCCAAAGAAGGCAGAAAAGGAA
ACCGAGTTATCGTAGTACAACACGGAATCGGAGAACACAGCGGTCGTTATGAATTTTTAGTGGAAGCACTTTCCGGAACC
GGAACGGCGTTCTATCTCATCGATTCTCAAGGGCATGGGCGCTCTGAAGGCAAACGAGGCGCGATAGATTCTTTTTCTGA
TTTCCTTTTCGATTTGGATCAATTGATTTCGATCGCAAAAGAAAAAGAAAAAGTTCCTAAAGTAACACTACTCGGTCATT
CTATGGGAGCCGCAATTTCTACATTCTATGCGGAAGAAGGTACTAATCAAGGAAATCTAAATGCTCTTATGATCTCCGCT
CTTCCGATTCGGGTAAAAACAGATCTAGTTATGAAAGTCAAAAAGGGAATTGCTCCTTTAATGTCCGATCTTTTACCCAA
CCTAACTCTTCCTACAGGACTCAACATTCACTTTTTAAGTCATGATAAGTCGGTAGTAGAAGCTTACAGAAAAGATCCAC
TGGTTCATGGAATGGCTTCTGCTTATTTAGGAAATATGCTTTTGAACAGCGAAGAACCAATTCTTGCAAATGCAGGTAAG
ATCAAAATTCCGATTTATATCTTCCACGGAAAAGAAGATCAGATCGCAGACTATACTGGAAGCGAAACCTTCTTCGAAGT
TGTAGGTTCCGCAGATAAGTCCATGAAAATTTACGAAGGACTTTATCACGAAACGATGAACGAACGTATAGAAGACAGAA
CTAAGGTTTTAACCGATTTAAAGAAATGGTTTGAATCTCACTCAAATTAA

Upstream 100 bases:

>100_bases
AAAAACATCCAGCAGCGGGCAAACAACATGAATCCAGAAAAATCGTTTTGTATCTTGAGACAACAGAAAAGAACCATTTC
TTAGGATGAATTTTTCCCAA

Downstream 100 bases:

>100_bases
CAGACAGAACGTTTGTTCTGTTTTTCGTTAAAAAAAGCATCGACCGGATTCTTCCGTTTTGGTACAATTGAATCCGGTCA
AAGACACTTTTTAAAAACAA

Product: lysophospholipase

Products: glycerophosphocholine; carboxylate

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKFFSLCKGSFAFITRFAMSWINSYNLKDDTFAGSGGSKIFYRTYQPKEGRKGNRVIVVQHGIGEHSGRYEFLVEALSGT
GTAFYLIDSQGHGRSEGKRGAIDSFSDFLFDLDQLISIAKEKEKVPKVTLLGHSMGAAISTFYAEEGTNQGNLNALMISA
LPIRVKTDLVMKVKKGIAPLMSDLLPNLTLPTGLNIHFLSHDKSVVEAYRKDPLVHGMASAYLGNMLLNSEEPILANAGK
IKIPIYIFHGKEDQIADYTGSETFFEVVGSADKSMKIYEGLYHETMNERIEDRTKVLTDLKKWFESHSN

Sequences:

>Translated_309_residues
MKFFSLCKGSFAFITRFAMSWINSYNLKDDTFAGSGGSKIFYRTYQPKEGRKGNRVIVVQHGIGEHSGRYEFLVEALSGT
GTAFYLIDSQGHGRSEGKRGAIDSFSDFLFDLDQLISIAKEKEKVPKVTLLGHSMGAAISTFYAEEGTNQGNLNALMISA
LPIRVKTDLVMKVKKGIAPLMSDLLPNLTLPTGLNIHFLSHDKSVVEAYRKDPLVHGMASAYLGNMLLNSEEPILANAGK
IKIPIYIFHGKEDQIADYTGSETFFEVVGSADKSMKIYEGLYHETMNERIEDRTKVLTDLKKWFESHSN
>Mature_309_residues
MKFFSLCKGSFAFITRFAMSWINSYNLKDDTFAGSGGSKIFYRTYQPKEGRKGNRVIVVQHGIGEHSGRYEFLVEALSGT
GTAFYLIDSQGHGRSEGKRGAIDSFSDFLFDLDQLISIAKEKEKVPKVTLLGHSMGAAISTFYAEEGTNQGNLNALMISA
LPIRVKTDLVMKVKKGIAPLMSDLLPNLTLPTGLNIHFLSHDKSVVEAYRKDPLVHGMASAYLGNMLLNSEEPILANAGK
IKIPIYIFHGKEDQIADYTGSETFFEVVGSADKSMKIYEGLYHETMNERIEDRTKVLTDLKKWFESHSN

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI51242953, Length=280, Percent_Identity=31.7857142857143, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI6005786, Length=275, Percent_Identity=32.3636363636364, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1787678, Length=262, Percent_Identity=26.3358778625954, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322756, Length=297, Percent_Identity=27.9461279461279, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: 3.1.1.5

Molecular weight: Translated: 34416; Mature: 34416

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFSLCKGSFAFITRFAMSWINSYNLKDDTFAGSGGSKIFYRTYQPKEGRKGNRVIVVQ
CCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
HGIGEHSGRYEFLVEALSGTGTAFYLIDSQGHGRSEGKRGAIDSFSDFLFDLDQLISIAK
CCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EKEKVPKVTLLGHSMGAAISTFYAEEGTNQGNLNALMISALPIRVKTDLVMKVKKGIAPL
HHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEEEEECEEEHHHHHHHHHHHHHHH
MSDLLPNLTLPTGLNIHFLSHDKSVVEAYRKDPLVHGMASAYLGNMLLNSEEPILANAGK
HHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCE
IKIPIYIFHGKEDQIADYTGSETFFEVVGSADKSMKIYEGLYHETMNERIEDRTKVLTDL
EEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKWFESHSN
HHHHHCCCC
>Mature Secondary Structure
MKFFSLCKGSFAFITRFAMSWINSYNLKDDTFAGSGGSKIFYRTYQPKEGRKGNRVIVVQ
CCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
HGIGEHSGRYEFLVEALSGTGTAFYLIDSQGHGRSEGKRGAIDSFSDFLFDLDQLISIAK
CCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EKEKVPKVTLLGHSMGAAISTFYAEEGTNQGNLNALMISALPIRVKTDLVMKVKKGIAPL
HHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEEEEECEEEHHHHHHHHHHHHHHH
MSDLLPNLTLPTGLNIHFLSHDKSVVEAYRKDPLVHGMASAYLGNMLLNSEEPILANAGK
HHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCE
IKIPIYIFHGKEDQIADYTGSETFFEVVGSADKSMKIYEGLYHETMNERIEDRTKVLTDL
EEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKWFESHSN
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-lysophosphatidylcholine; H2O

Specific reaction: 2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]