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The map label for this gene is wcaJ [H]

Identifier: 45656235

GI number: 45656235

Start: 376109

End: 377497

Strand: Reverse

Name: wcaJ [H]

Synonym: LIC10331

Alternate gene names: 45656235

Gene position: 377497-376109 (Counterclockwise)

Preceding gene: 45656240

Following gene: 45656234

Centisome position: 8.83

GC content: 32.9

Gene sequence:

>1389_bases
ATGCATTCTTTCAAAAACTTTTATATATTCTCAAACATGAAAAGAATCATCGAAATCGTTTTATCCACCCTCGCTTTAAT
TTTATTCTCTCCAATTTTGATCGGAGTAAGTTTTTTAATTCTTTTGATCAATGGTCTTCCAATTTTCTTTTTTCAGGAAC
GACTCGGTCTTTTAAAAAAACCGTTTCTAATTTTTAAATTTAGAACGATGAAAGATGGAAAAGTTACAAACCTAGGTTAT
TGGTTGAGAAAAACCGGCATTGACGAACTTCCTCAAATTTGGAATATCTTAATCGGAAATATGGGAATTGTTGGTCCAAG
ACCACTCACTCAACTTGACATAGATCGTTTAAATTGGAATGGGAAATTTCACGAAATGCGTTGGTCCGTTCTTCCTGGTA
TCACAGGACTTTCGCAGTTGTATTCCGGTATGGGTGCGCGTATTTCCTTTTGTTTTGATCGTTTTTATTTGGAATCAAAA
AATCTAGGACTTAACATTCTTATCGTTCTATTGACTTTCGTCATAAATTTATTTGGTAAGAATAGAATCCGAGAAAAGCT
CAAATCAAAACTTAAAACTAGAAAAAACAAAATCCAATGGAAACATTGGAAAAATCATTTTAAAAAAAATGAAAATCGTA
TCTTGCCTAAAATCGATTTCGAAACTTTAGAACTTAACTCAAATGAAATGCGGTCAATCGCTTACTCGCTCGCTATATTC
CAGTTAGGTGAATCTGGGGAAGGTAGAATCGCAAAAGAAATAGACAAAACAATTCTTTTCGGAATTGACGATTTTTATAA
AGAAGCGTTAAAACTTTTTGTAAAAGAGGAAGGTAGACACGCCAGAATTTTAGGAGAATGTATTCGAGCTCTCAAAGGAG
AATTGATCAAATCCAATTGGACGGAAAAGTTATTTCATTTTGGTAGAAGATTACTCGGAATCCGCTTGAAGTTAATGGTT
CTTCTTGCTGCGGAAGTTGTAGGAATTTGTTTTTATAAAAAACTTTCTGAAAAAATTCCAAACGGATTTATAAAATCTGC
CTTACTTGAAATTGTAAAAGACGAAGAGAAACATCTTAGATTTCACGGAGATTTTTTCAGAACTCAGGTCAGAAATTTTT
TCAGTAAATTCGTTTTTAGATTGTTATGGAGATTCGTCGCATTCGCTGCTTGTGTCGCAGTGATTTTAGATCATAGAAAA
ACTTTTTGTATATTAGGAATTTCTAATTGGAAAACTTTTCTGAAATTTCAAGAGATTGCGAAAGCCACAGAGGACTTTAT
CACGGAAGGTTTAGAGTTTTATTCCCATACCTCAAAATATAGGAACCTATACGAAAATTTAAATGATATTCAAACTACTT
ACTACTCTACGCATGAAAATGGTATCTGA

Upstream 100 bases:

>100_bases
GATTTAAAACTTTTTCTTTCATAGATAAAAAACCAGATTCTATGTTTCTTTATTAAACTGTGTCAAGCACTTGAGTGCTT
TATAATTTGCACTCAAGAGG

Downstream 100 bases:

>100_bases
AATTAACCGTCAATTCTACAAAAATGCGGAAACCCTCAAAAATTGAATCTAATCTGGAATTGTTAGCCGTTTTTAAAGAA
GATCATATATTCTAATTTTT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MHSFKNFYIFSNMKRIIEIVLSTLALILFSPILIGVSFLILLINGLPIFFFQERLGLLKKPFLIFKFRTMKDGKVTNLGY
WLRKTGIDELPQIWNILIGNMGIVGPRPLTQLDIDRLNWNGKFHEMRWSVLPGITGLSQLYSGMGARISFCFDRFYLESK
NLGLNILIVLLTFVINLFGKNRIREKLKSKLKTRKNKIQWKHWKNHFKKNENRILPKIDFETLELNSNEMRSIAYSLAIF
QLGESGEGRIAKEIDKTILFGIDDFYKEALKLFVKEEGRHARILGECIRALKGELIKSNWTEKLFHFGRRLLGIRLKLMV
LLAAEVVGICFYKKLSEKIPNGFIKSALLEIVKDEEKHLRFHGDFFRTQVRNFFSKFVFRLLWRFVAFAACVAVILDHRK
TFCILGISNWKTFLKFQEIAKATEDFITEGLEFYSHTSKYRNLYENLNDIQTTYYSTHENGI

Sequences:

>Translated_462_residues
MHSFKNFYIFSNMKRIIEIVLSTLALILFSPILIGVSFLILLINGLPIFFFQERLGLLKKPFLIFKFRTMKDGKVTNLGY
WLRKTGIDELPQIWNILIGNMGIVGPRPLTQLDIDRLNWNGKFHEMRWSVLPGITGLSQLYSGMGARISFCFDRFYLESK
NLGLNILIVLLTFVINLFGKNRIREKLKSKLKTRKNKIQWKHWKNHFKKNENRILPKIDFETLELNSNEMRSIAYSLAIF
QLGESGEGRIAKEIDKTILFGIDDFYKEALKLFVKEEGRHARILGECIRALKGELIKSNWTEKLFHFGRRLLGIRLKLMV
LLAAEVVGICFYKKLSEKIPNGFIKSALLEIVKDEEKHLRFHGDFFRTQVRNFFSKFVFRLLWRFVAFAACVAVILDHRK
TFCILGISNWKTFLKFQEIAKATEDFITEGLEFYSHTSKYRNLYENLNDIQTTYYSTHENGI
>Mature_462_residues
MHSFKNFYIFSNMKRIIEIVLSTLALILFSPILIGVSFLILLINGLPIFFFQERLGLLKKPFLIFKFRTMKDGKVTNLGY
WLRKTGIDELPQIWNILIGNMGIVGPRPLTQLDIDRLNWNGKFHEMRWSVLPGITGLSQLYSGMGARISFCFDRFYLESK
NLGLNILIVLLTFVINLFGKNRIREKLKSKLKTRKNKIQWKHWKNHFKKNENRILPKIDFETLELNSNEMRSIAYSLAIF
QLGESGEGRIAKEIDKTILFGIDDFYKEALKLFVKEEGRHARILGECIRALKGELIKSNWTEKLFHFGRRLLGIRLKLMV
LLAAEVVGICFYKKLSEKIPNGFIKSALLEIVKDEEKHLRFHGDFFRTQVRNFFSKFVFRLLWRFVAFAACVAVILDHRK
TFCILGISNWKTFLKFQEIAKATEDFITEGLEFYSHTSKYRNLYENLNDIQTTYYSTHENGI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=181, Percent_Identity=35.3591160220994, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54146; Mature: 54146

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSFKNFYIFSNMKRIIEIVLSTLALILFSPILIGVSFLILLINGLPIFFFQERLGLLKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
PFLIFKFRTMKDGKVTNLGYWLRKTGIDELPQIWNILIGNMGIVGPRPLTQLDIDRLNWN
CHHEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
GKFHEMRWSVLPGITGLSQLYSGMGARISFCFDRFYLESKNLGLNILIVLLTFVINLFGK
CCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCH
NRIREKLKSKLKTRKNKIQWKHWKNHFKKNENRILPKIDFETLELNSNEMRSIAYSLAIF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
QLGESGEGRIAKEIDKTILFGIDDFYKEALKLFVKEEGRHARILGECIRALKGELIKSNW
HCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCH
TEKLFHFGRRLLGIRLKLMVLLAAEVVGICFYKKLSEKIPNGFIKSALLEIVKDEEKHLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHH
FHGDFFRTQVRNFFSKFVFRLLWRFVAFAACVAVILDHRKTFCILGISNWKTFLKFQEIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHH
KATEDFITEGLEFYSHTSKYRNLYENLNDIQTTYYSTHENGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHSFKNFYIFSNMKRIIEIVLSTLALILFSPILIGVSFLILLINGLPIFFFQERLGLLKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
PFLIFKFRTMKDGKVTNLGYWLRKTGIDELPQIWNILIGNMGIVGPRPLTQLDIDRLNWN
CHHEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
GKFHEMRWSVLPGITGLSQLYSGMGARISFCFDRFYLESKNLGLNILIVLLTFVINLFGK
CCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCH
NRIREKLKSKLKTRKNKIQWKHWKNHFKKNENRILPKIDFETLELNSNEMRSIAYSLAIF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
QLGESGEGRIAKEIDKTILFGIDDFYKEALKLFVKEEGRHARILGECIRALKGELIKSNW
HCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCH
TEKLFHFGRRLLGIRLKLMVLLAAEVVGICFYKKLSEKIPNGFIKSALLEIVKDEEKHLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHH
FHGDFFRTQVRNFFSKFVFRLLWRFVAFAACVAVILDHRKTFCILGISNWKTFLKFQEIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHH
KATEDFITEGLEFYSHTSKYRNLYENLNDIQTTYYSTHENGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]