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The map label for this gene is hemK

Identifier: 45656171

GI number: 45656171

Start: 302883

End: 303743

Strand: Reverse

Name: hemK

Synonym: LIC10266

Alternate gene names: 45656171

Gene position: 303743-302883 (Counterclockwise)

Preceding gene: 45656172

Following gene: 45656166

Centisome position: 7.1

GC content: 33.91

Gene sequence:

>861_bases
ATGCAACACCCAGATTCAATTCTCACTCTTTTAAAAAAATCGGAAGAATTCTTAAAAAAGAAAGAAATTCCAAGCGCACG
TTTAGATGCAGAAATTCTTTTAGCCGATCTACTCAATCTCCAAAGAGTAAAACTTTACGTAAACTTTGAAAGACTTTTAA
ACGAAACAGAAAAAAACGCATATAGAGAAAGAATTTTAGAAAGATCTAAAAATAAACCCACTGCTTACATCACAAGCCAA
AAGGCGTTTTACAATTCTATATTTTTTGTGAACGAAAATGTACTTATACCAAGACCAGAAACGGAAGAACTCGTGGAAAA
GGTTTTATCGGATTTTAAAGGAAATATAGGAGAGCAAAATGTATTAGACCTTTGTACCGGAAGCGGTTGTATCGGAATCA
GTTTAAAACTTGCTCGGAAAGACTGGAACATTACATTAAGTGACATTTCAAAAGAAGCGTTAGAAGTTGCTGCAAAAAAC
GCTATTCAAATTTTGGGAGAGGAAAAACACATTCAATTTTTAGAAAGTGATTTGTTTCTTTCAATTCCTAAAGAATCCAA
ATTTAATCTGATCGTTACCAACCCACCTTATATCCCTATTTCAGATAAAGCAGAAATGATGAAAGATGTAATAGATTATG
AGCCCCACCTCGCTTTATTTCTAGAAAATCCGAAAGACTTTTTATCTACTCTGATCGCACAGGCACATGAACGATTGGTC
GAAGGTGGAAAACTTTATATGGAGATTTTACCTTCTCTATCCGAAACTATTATTTCGGATTCAATCGCAAAAGGTTGGGA
GAATGGAAAAATCGAGAAAGATCTTTCCGGAAAAGAACGGTTTGTAATTTTAACAAGATAA

Upstream 100 bases:

>100_bases
CTTATATTTTACAAAAATCCGAATTGGGTTTGAAAAAATGAAATCCACTGTAACGAAAACTCTTAAACTGGCACTAATAA
ACAGTCACACCGAATTATAG

Downstream 100 bases:

>100_bases
AATCGGCATTTTAAACAAAGACAAAGTATTTTCTAGATAGGTTCTTAGAACTTACCCCGAAATACATAAACGATTATTTT
TTAAAAATCTCTTTTACGAC

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQHPDSILTLLKKSEEFLKKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLNETEKNAYRERILERSKNKPTAYITSQ
KAFYNSIFFVNENVLIPRPETEELVEKVLSDFKGNIGEQNVLDLCTGSGCIGISLKLARKDWNITLSDISKEALEVAAKN
AIQILGEEKHIQFLESDLFLSIPKESKFNLIVTNPPYIPISDKAEMMKDVIDYEPHLALFLENPKDFLSTLIAQAHERLV
EGGKLYMEILPSLSETIISDSIAKGWENGKIEKDLSGKERFVILTR

Sequences:

>Translated_286_residues
MQHPDSILTLLKKSEEFLKKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLNETEKNAYRERILERSKNKPTAYITSQ
KAFYNSIFFVNENVLIPRPETEELVEKVLSDFKGNIGEQNVLDLCTGSGCIGISLKLARKDWNITLSDISKEALEVAAKN
AIQILGEEKHIQFLESDLFLSIPKESKFNLIVTNPPYIPISDKAEMMKDVIDYEPHLALFLENPKDFLSTLIAQAHERLV
EGGKLYMEILPSLSETIISDSIAKGWENGKIEKDLSGKERFVILTR
>Mature_286_residues
MQHPDSILTLLKKSEEFLKKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLNETEKNAYRERILERSKNKPTAYITSQ
KAFYNSIFFVNENVLIPRPETEELVEKVLSDFKGNIGEQNVLDLCTGSGCIGISLKLARKDWNITLSDISKEALEVAAKN
AIQILGEEKHIQFLESDLFLSIPKESKFNLIVTNPPYIPISDKAEMMKDVIDYEPHLALFLENPKDFLSTLIAQAHERLV
EGGKLYMEILPSLSETIISDSIAKGWENGKIEKDLSGKERFVILTR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=307, Percent_Identity=25.4071661237785, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787463, Length=277, Percent_Identity=31.0469314079422, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082085, Length=250, Percent_Identity=32.8, Blast_Score=105, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24582226, Length=257, Percent_Identity=28.7937743190661, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32623; Mature: 32623

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHPDSILTLLKKSEEFLKKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLNETEKNA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHEEEEHHHHHHHHHHHH
YRERILERSKNKPTAYITSQKAFYNSIFFVNENVLIPRPETEELVEKVLSDFKGNIGEQN
HHHHHHHHCCCCCEEEEECCHHHHHEEEEEECCEEECCCCHHHHHHHHHHHHCCCCCCCH
VLDLCTGSGCIGISLKLARKDWNITLSDISKEALEVAAKNAIQILGEEKHIQFLESDLFL
HHHHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
SIPKESKFNLIVTNPPYIPISDKAEMMKDVIDYEPHLALFLENPKDFLSTLIAQAHERLV
ECCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHH
EGGKLYMEILPSLSETIISDSIAKGWENGKIEKDLSGKERFVILTR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MQHPDSILTLLKKSEEFLKKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLNETEKNA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHEEEEHHHHHHHHHHHH
YRERILERSKNKPTAYITSQKAFYNSIFFVNENVLIPRPETEELVEKVLSDFKGNIGEQN
HHHHHHHHCCCCCEEEEECCHHHHHEEEEEECCEEECCCCHHHHHHHHHHHHCCCCCCCH
VLDLCTGSGCIGISLKLARKDWNITLSDISKEALEVAAKNAIQILGEEKHIQFLESDLFL
HHHHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
SIPKESKFNLIVTNPPYIPISDKAEMMKDVIDYEPHLALFLENPKDFLSTLIAQAHERLV
ECCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHH
EGGKLYMEILPSLSETIISDSIAKGWENGKIEKDLSGKERFVILTR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]