Definition Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession NC_005823
Length 4,277,185

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The map label for this gene is fumC

Identifier: 45656068

GI number: 45656068

Start: 193574

End: 194968

Strand: Direct

Name: fumC

Synonym: LIC10162

Alternate gene names: 45656068

Gene position: 193574-194968 (Clockwise)

Preceding gene: 45656067

Following gene: 45656074

Centisome position: 4.53

GC content: 40.93

Gene sequence:

>1395_bases
ATGAAAACCAGAATCGAAACGGATTCGATGGGCGAAATTGCAGTAGACGATTCCAAATATTGGGGCGCACAAACAGAACG
TTCCTTACATCATTTTCATATCGGAAACGATCGATTTCCAAGAGAAATGATTCGTGCTTTAGGAATTTTAAAGAAATCTG
CAGCGGTTGTTAATGCGGAACTTGGTCTTTTAAGCGAAGATAAAAAGAAACTGATTGTTCAAGCCGCCGACGAAGTGATT
TCCGGAAAATTGGATGAGCATTTTCCGCTTTCGGTTTGGCAGACCGGCTCCGGAACTCAGACTAACATGAATTCTAATGA
AGTGATTTCGAACCGTGCGATCGAAATTGCAGGTGGGGTGAAGGGCTCTAAAAAACCGATTCATCCAAATGACGATGTTA
ACAAGGCTCAATCCTCAAACGATACGTTTCCTACAGCCATGCATATCGCTGCGGCGGAACAGTTAAACCAAAAGTTGATT
CCTGCTCTGATACAACTCAAAGAAACGTTCAAGAAAAAAACAGATGAATTTCAGAATATTATAAAAATCGGTAGAACTCA
TCTTCAAGACGCGACTCCTCTGACTTTAGGCCAAGAATTTTCTGGCTATGTTCAACAATTGGAATACAATATTGCAAGGG
TAAAGGCAGTTTTACCTTCCGTTTATAGACTTGCGTTAGGCGGAACAGCTGTCGGAACCGGATTAAATACTCACCCTCAG
TTTGCGGTAAAAGCAGCGGCTCAAATCGCTAAAGAAACTGGACTTCCTTTTGTAAGCGCGGAAAATAAGTTCGAGGCACT
CGCGGCTCACGATTCTCTTGTAGAAACGAGCGGTGTGTTGAAAACGATCGCTGCTTCTTTAATGAAAATTGCAAACGATA
TACGTTGGCTTTCTTCTGGTCCTCGTTGTGGAATTGGTGAAATTAGTATTCCGGAAAATGAACCAGGTTCTTCTATCATG
CCGGGTAAAGTGAATCCAACACAATCAGAGCAGATGACGATGGTCGCGGCTCAAGTAATTGCAAATGACGTTGCAGTAAA
TATAGGCGGTGCTTCTGGAAATTTCGAGTTGAACGTTTTTAAACCTTTGATTATCCACAACGTTTTGAATTCGATTCGAT
TACTTTCTGATTCTTGTGTTTCTTTTGAAGAACATTGTGCTCGTGGAATTATTCCGAATAAGGAAAAGTTAAACGAACAC
TTAAATAATTCTTTGATGCTTGTGACCGCTTTGAATCCTCATATAGGTTATGATAACGCTGCTAAAATTGCCAAGAACGC
GCATAAAAAAGGAACAACTCTGAAAGAGTCCGGAATTGAATTGGGACTTTTAACAAGTGAACAATTTGATCAGTGGGTTC
TTCCCGAAAAGATGATCCATCCTTCGGTGGATTAA

Upstream 100 bases:

>100_bases
TCCAAAACGAATGAAAATTACATTAGAATTTTATTAAATTCTAATATTTTATTTAAAAAGATTTTTTATTTCAATTAATA
TTTAAGTTTTCGGAGGATTC

Downstream 100 bases:

>100_bases
TTCAAGGTGTTAGGCGAAGGGAAGTTAGTCCCTTCGCTTATCTAATTTTTTGATCGTTTTCGTTTCAACTTCAAAAAAAT
ATTATATTTTTAAAAAGTAG

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKTRIETDSMGEIAVDDSKYWGAQTERSLHHFHIGNDRFPREMIRALGILKKSAAVVNAELGLLSEDKKKLIVQAADEVI
SGKLDEHFPLSVWQTGSGTQTNMNSNEVISNRAIEIAGGVKGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEQLNQKLI
PALIQLKETFKKKTDEFQNIIKIGRTHLQDATPLTLGQEFSGYVQQLEYNIARVKAVLPSVYRLALGGTAVGTGLNTHPQ
FAVKAAAQIAKETGLPFVSAENKFEALAAHDSLVETSGVLKTIAASLMKIANDIRWLSSGPRCGIGEISIPENEPGSSIM
PGKVNPTQSEQMTMVAAQVIANDVAVNIGGASGNFELNVFKPLIIHNVLNSIRLLSDSCVSFEEHCARGIIPNKEKLNEH
LNNSLMLVTALNPHIGYDNAAKIAKNAHKKGTTLKESGIELGLLTSEQFDQWVLPEKMIHPSVD

Sequences:

>Translated_464_residues
MKTRIETDSMGEIAVDDSKYWGAQTERSLHHFHIGNDRFPREMIRALGILKKSAAVVNAELGLLSEDKKKLIVQAADEVI
SGKLDEHFPLSVWQTGSGTQTNMNSNEVISNRAIEIAGGVKGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEQLNQKLI
PALIQLKETFKKKTDEFQNIIKIGRTHLQDATPLTLGQEFSGYVQQLEYNIARVKAVLPSVYRLALGGTAVGTGLNTHPQ
FAVKAAAQIAKETGLPFVSAENKFEALAAHDSLVETSGVLKTIAASLMKIANDIRWLSSGPRCGIGEISIPENEPGSSIM
PGKVNPTQSEQMTMVAAQVIANDVAVNIGGASGNFELNVFKPLIIHNVLNSIRLLSDSCVSFEEHCARGIIPNKEKLNEH
LNNSLMLVTALNPHIGYDNAAKIAKNAHKKGTTLKESGIELGLLTSEQFDQWVLPEKMIHPSVD
>Mature_464_residues
MKTRIETDSMGEIAVDDSKYWGAQTERSLHHFHIGNDRFPREMIRALGILKKSAAVVNAELGLLSEDKKKLIVQAADEVI
SGKLDEHFPLSVWQTGSGTQTNMNSNEVISNRAIEIAGGVKGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEQLNQKLI
PALIQLKETFKKKTDEFQNIIKIGRTHLQDATPLTLGQEFSGYVQQLEYNIARVKAVLPSVYRLALGGTAVGTGLNTHPQ
FAVKAAAQIAKETGLPFVSAENKFEALAAHDSLVETSGVLKTIAASLMKIANDIRWLSSGPRCGIGEISIPENEPGSSIM
PGKVNPTQSEQMTMVAAQVIANDVAVNIGGASGNFELNVFKPLIIHNVLNSIRLLSDSCVSFEEHCARGIIPNKEKLNEH
LNNSLMLVTALNPHIGYDNAAKIAKNAHKKGTTLKESGIELGLLTSEQFDQWVLPEKMIHPSVD

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=65.8008658008658, Blast_Score=605, Evalue=1e-173,
Organism=Homo sapiens, GI266458395, Length=77, Percent_Identity=49.3506493506493, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1787896, Length=456, Percent_Identity=63.3771929824561, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=40.04329004329, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17553882, Length=464, Percent_Identity=65.948275862069, Blast_Score=627, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=66.8711656441718, Blast_Score=465, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=63.695652173913, Blast_Score=597, Evalue=1e-171,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=65.29284164859, Blast_Score=610, Evalue=1e-175,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=65.29284164859, Blast_Score=610, Evalue=1e-175,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=62.5, Blast_Score=584, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24662684, Length=468, Percent_Identity=61.965811965812, Blast_Score=583, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=54.6436285097192, Blast_Score=496, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FUMC_LEPIC (Q72VY3)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000154.1
- ProteinModelPortal:   Q72VY3
- SMR:   Q72VY3
- GeneID:   2771729
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10162
- HOGENOM:   HBG284369
- OMA:   RIEKDTM
- ProtClustDB:   PRK00485
- BioCyc:   LINT267671:LIC_10162-MONOMER
- HAMAP:   MF_00743
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00979

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.2.1.2

Molecular weight: Translated: 50397; Mature: 50397

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MKTRIETDSMGEIAVDDSKYWGAQTERSLHHFHIGNDRFPREMIRALGILKKSAAVVNAE
CCCCCCCCCCCCEEECCCHHCCCHHHCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LGLLSEDKKKLIVQAADEVISGKLDEHFPLSVWQTGSGTQTNMNSNEVISNRAIEIAGGV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCEEEECCC
KGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEQLNQKLIPALIQLKETFKKKTDEFQNI
CCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKIGRTHLQDATPLTLGQEFSGYVQQLEYNIARVKAVLPSVYRLALGGTAVGTGLNTHPQ
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
FAVKAAAQIAKETGLPFVSAENKFEALAAHDSLVETSGVLKTIAASLMKIANDIRWLSSG
HHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRCGIGEISIPENEPGSSIMPGKVNPTQSEQMTMVAAQVIANDVAVNIGGASGNFELNVF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEH
KPLIIHNVLNSIRLLSDSCVSFEEHCARGIIPNKEKLNEHLNNSLMLVTALNPHIGYDNA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHH
AKIAKNAHKKGTTLKESGIELGLLTSEQFDQWVLPEKMIHPSVD
HHHHHHHHHCCCCHHHCCCEEEEEEHHHHHHHCCCHHHCCCCCC
>Mature Secondary Structure
MKTRIETDSMGEIAVDDSKYWGAQTERSLHHFHIGNDRFPREMIRALGILKKSAAVVNAE
CCCCCCCCCCCCEEECCCHHCCCHHHCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LGLLSEDKKKLIVQAADEVISGKLDEHFPLSVWQTGSGTQTNMNSNEVISNRAIEIAGGV
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCEEEECCC
KGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEQLNQKLIPALIQLKETFKKKTDEFQNI
CCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKIGRTHLQDATPLTLGQEFSGYVQQLEYNIARVKAVLPSVYRLALGGTAVGTGLNTHPQ
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
FAVKAAAQIAKETGLPFVSAENKFEALAAHDSLVETSGVLKTIAASLMKIANDIRWLSSG
HHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRCGIGEISIPENEPGSSIMPGKVNPTQSEQMTMVAAQVIANDVAVNIGGASGNFELNVF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEH
KPLIIHNVLNSIRLLSDSCVSFEEHCARGIIPNKEKLNEHLNNSLMLVTALNPHIGYDNA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCCCCHH
AKIAKNAHKKGTTLKESGIELGLLTSEQFDQWVLPEKMIHPSVD
HHHHHHHHHCCCCHHHCCCEEEEEEHHHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA