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The map label for this gene is thiD

Identifier: 45656028

GI number: 45656028

Start: 137048

End: 137869

Strand: Reverse

Name: thiD

Synonym: LIC10118

Alternate gene names: 45656028

Gene position: 137869-137048 (Counterclockwise)

Preceding gene: 45656029

Following gene: 45656020

Centisome position: 3.22

GC content: 34.18

Gene sequence:

>822_bases
ATGGATAATGAAAACATCAAACCAGTCACACTGACGATTGCGGGTTCGGATTCTGGAGGAGGAGCCGGAATTCAAGCAGA
TTTAAAAACATTTACAGCTCTGGGTACTTTCGGAACTTCCGCAATTACTTGTCTAACTTCTCAAAATCCTTCGAGTGTTA
CTGGAATTTTAGAAGTGAATGCAAATTTTTTAGAAAAACAAATCTTGGCAGTTCTGAATTATTTTCCAGTGAAAGCAATT
AAAACCGGAATGTTATTTTCTACATCTATTATTGAAACAACGAGTTCTATTCTTTCAAAAAGAAAGAAAGAGGAAAACCA
TTTTTTTTTAGTAATCGATCCAGTAATGGTAGCGACAAGCGGCGCAAAACTTTTACAAGATTCTGCGATCGATGTTTTGT
TAACAAAATTAATCCCGATTGGAAACTTAATTACACCTAACCTAGATGAAGCAGAAATTCTTTCCGGAAAAAAAATAAAT
ACAAGTGCGGAAATGCCTGATCTTGCAAAAGAAATATTCGAAAAGTTTAAAGTCCCAACTCTTTTAAAAGGAGGTCATTT
ACAAAACGAGAAAGTTGCGATCGACGTTTTGTATGATGGTAAAAAAATTTCTAAATTTGAAAAACCGTTTGTAAATGGGT
TTTATCCACATGGAACAGGATGTACGTATTCTTCCGCAATTGCTTCTTATCTAGCTCTAGGAAAAAATTTGATCGAAGCA
ATTGATTATGCAAAAGAATATTTACACGCAGCCATTGAACAATCCTATATCGCGGGAAAAGATAAAACTCTTAATCACAC
TCCGTTATTTCATAAAACTTAA

Upstream 100 bases:

>100_bases
AACGCTGGTTCCATACAAAATATTAGGCACTTTATTATATAGTTATGAATAGTATTCGATAAATAAAAGATTCATCTTAA
AACTACATTAGAGAAATTGA

Downstream 100 bases:

>100_bases
AATCAATTTCAAAATGTTGCTGATACTGAAACCATCTACAGAACCCAATAAAAACTAATAATTAGCTCTTGTTAAAATAA
AAAAATTGAGATAGGTTTTG

Product: phoshomethylpyrimidine kinase protein

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MDNENIKPVTLTIAGSDSGGGAGIQADLKTFTALGTFGTSAITCLTSQNPSSVTGILEVNANFLEKQILAVLNYFPVKAI
KTGMLFSTSIIETTSSILSKRKKEENHFFLVIDPVMVATSGAKLLQDSAIDVLLTKLIPIGNLITPNLDEAEILSGKKIN
TSAEMPDLAKEIFEKFKVPTLLKGGHLQNEKVAIDVLYDGKKISKFEKPFVNGFYPHGTGCTYSSAIASYLALGKNLIEA
IDYAKEYLHAAIEQSYIAGKDKTLNHTPLFHKT

Sequences:

>Translated_273_residues
MDNENIKPVTLTIAGSDSGGGAGIQADLKTFTALGTFGTSAITCLTSQNPSSVTGILEVNANFLEKQILAVLNYFPVKAI
KTGMLFSTSIIETTSSILSKRKKEENHFFLVIDPVMVATSGAKLLQDSAIDVLLTKLIPIGNLITPNLDEAEILSGKKIN
TSAEMPDLAKEIFEKFKVPTLLKGGHLQNEKVAIDVLYDGKKISKFEKPFVNGFYPHGTGCTYSSAIASYLALGKNLIEA
IDYAKEYLHAAIEQSYIAGKDKTLNHTPLFHKT
>Mature_273_residues
MDNENIKPVTLTIAGSDSGGGAGIQADLKTFTALGTFGTSAITCLTSQNPSSVTGILEVNANFLEKQILAVLNYFPVKAI
KTGMLFSTSIIETTSSILSKRKKEENHFFLVIDPVMVATSGAKLLQDSAIDVLLTKLIPIGNLITPNLDEAEILSGKKIN
TSAEMPDLAKEIFEKFKVPTLLKGGHLQNEKVAIDVLYDGKKISKFEKPFVNGFYPHGTGCTYSSAIASYLALGKNLIEA
IDYAKEYLHAAIEQSYIAGKDKTLNHTPLFHKT

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=246, Percent_Identity=38.2113821138211, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325378, Length=259, Percent_Identity=34.7490347490347, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=278, Percent_Identity=35.2517985611511, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324997, Length=278, Percent_Identity=35.2517985611511, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29516; Mature: 29516

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNENIKPVTLTIAGSDSGGGAGIQADLKTFTALGTFGTSAITCLTSQNPSSVTGILEVN
CCCCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEC
ANFLEKQILAVLNYFPVKAIKTGMLFSTSIIETTSSILSKRKKEENHFFLVIDPVMVATS
HHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCC
GAKLLQDSAIDVLLTKLIPIGNLITPNLDEAEILSGKKINTSAEMPDLAKEIFEKFKVPT
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCC
LLKGGHLQNEKVAIDVLYDGKKISKFEKPFVNGFYPHGTGCTYSSAIASYLALGKNLIEA
EECCCCCCCCEEEEEEEECCCHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IDYAKEYLHAAIEQSYIAGKDKTLNHTPLFHKT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDNENIKPVTLTIAGSDSGGGAGIQADLKTFTALGTFGTSAITCLTSQNPSSVTGILEVN
CCCCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEC
ANFLEKQILAVLNYFPVKAIKTGMLFSTSIIETTSSILSKRKKEENHFFLVIDPVMVATS
HHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCC
GAKLLQDSAIDVLLTKLIPIGNLITPNLDEAEILSGKKINTSAEMPDLAKEIFEKFKVPT
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCC
LLKGGHLQNEKVAIDVLYDGKKISKFEKPFVNGFYPHGTGCTYSSAIASYLALGKNLIEA
EECCCCCCCCEEEEEEEECCCHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IDYAKEYLHAAIEQSYIAGKDKTLNHTPLFHKT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA