BacMap

Definition	Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession	NC_005823
Length	4,277,185

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The map label for this gene is yngF [H]

Identifier: 45655980

GI number: 45655980

Start: 84866

End: 85648

Strand: Reverse

Name: yngF [H]

Synonym: LIC10066

Alternate gene names: 45655980

Gene position: 85648-84866 (Counterclockwise)

Preceding gene: 45655983

Following gene: 45655979

Centisome position: 2.0

GC content: 37.93

Gene sequence:

>783_bases
ATGAACGAATTTTCTACAGTATTGTATTCCACGGAAAAAGAAATCGCGGTTCTACTTCTAAATAGACCGGAAAAAAGAAA
CGCAATCAGTAAAGAACTTCTTTCTACACTTCATAAAAATATTCTCAAAGCAAAAAAGGAAAAATCAATCCGTAGTCTTG
TTCTAAGCGGCGTTGGACCTTCCTTTTGTGCAGGAGCAGACTTAAAAGAAAGAGTAACCATGTCTCCTAAAGAAGTGAAA
CGTTTTTTAGAAGATCTCAAAAATTGTTTTTTGGAATTGGAAAACTTTCCATATCCTACGGTTGCCGCCTTAGACGGAGA
CGCATTTGGAGGAGGATTGGAACTTGCACTTTGTTGTGATTTGATTCTTCTTAAAAACGATATTAGAATTGGACTTACAG
AAACTCGTTTAGGAATTATTCCTGGTGGAGGAGGAACTCAAAGACTTTCACGTAGAATCGGAATCTCGAAAGCGAAAGAA
ATGATTTTTACGGGCAAAACAATTGACGCACAAACCGCTTTAGATTTTGGAATCGCAAATTCCATTTGGCACGATTCCTC
CCTTCCCGCAGCAAAAATGTTAGCTGAAGAAATCGCATCTCAATGTGCACCAATCGCACTTCAGCTTGCAAAGAAAGCAA
TTACAGAAGGTTATGGTCAGGACATACGAAAAGCTCTCATAACCGAGAGTAAATACTATAATAACACTTTAAATACGGAA
GATCGGTTGGAGGCACTCAAAGCGTTTCAGGAAAAACGAAAACCGATTTTTAAAGGAAAATAA

Upstream 100 bases:

>100_bases
ATAACTTTATTTGATCTAAAAAATCTAATACGGGCCTCTAGTTTATAAAAGTAAAAAGGTTTCGGCAAGAATTATTCTGC
TGAAAAAATGGTGTACTGAA

Downstream 100 bases:

>100_bases
ATGATTCTTACTGGAAAAGAAATTCAAAAACGAATCGGTAATGATATTGTAATTACTCCTTATTCAGAAAAACAACTCAA
TCCAAATTCTTACAATCTAA

Product: enoyl-CoA hydratase/isomerase family protein

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNEFSTVLYSTEKEIAVLLLNRPEKRNAISKELLSTLHKNILKAKKEKSIRSLVLSGVGPSFCAGADLKERVTMSPKEVK
RFLEDLKNCFLELENFPYPTVAALDGDAFGGGLELALCCDLILLKNDIRIGLTETRLGIIPGGGGTQRLSRRIGISKAKE
MIFTGKTIDAQTALDFGIANSIWHDSSLPAAKMLAEEIASQCAPIALQLAKKAITEGYGQDIRKALITESKYYNNTLNTE
DRLEALKAFQEKRKPIFKGK

Sequences:

>Translated_260_residues
MNEFSTVLYSTEKEIAVLLLNRPEKRNAISKELLSTLHKNILKAKKEKSIRSLVLSGVGPSFCAGADLKERVTMSPKEVK
RFLEDLKNCFLELENFPYPTVAALDGDAFGGGLELALCCDLILLKNDIRIGLTETRLGIIPGGGGTQRLSRRIGISKAKE
MIFTGKTIDAQTALDFGIANSIWHDSSLPAAKMLAEEIASQCAPIALQLAKKAITEGYGQDIRKALITESKYYNNTLNTE
DRLEALKAFQEKRKPIFKGK
>Mature_260_residues
MNEFSTVLYSTEKEIAVLLLNRPEKRNAISKELLSTLHKNILKAKKEKSIRSLVLSGVGPSFCAGADLKERVTMSPKEVK
RFLEDLKNCFLELENFPYPTVAALDGDAFGGGLELALCCDLILLKNDIRIGLTETRLGIIPGGGGTQRLSRRIGISKAKE
MIFTGKTIDAQTALDFGIANSIWHDSSLPAAKMLAEEIASQCAPIALQLAKKAITEGYGQDIRKALITESKYYNNTLNTE
DRLEALKAFQEKRKPIFKGK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=42.4710424710425, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI150378541, Length=263, Percent_Identity=36.1216730038023, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=35.2226720647773, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=27.6859504132231, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=28.957528957529, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=34.7593582887701, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI20127408, Length=263, Percent_Identity=30.4182509505703, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=25.7383966244726, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=25.7383966244726, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=26.1467889908257, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=26.1467889908257, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=26.1467889908257, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI116235487, Length=209, Percent_Identity=23.444976076555, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157694520, Length=201, Percent_Identity=25.8706467661692, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI261878539, Length=94, Percent_Identity=38.2978723404255, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI62530384, Length=171, Percent_Identity=30.9941520467836, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=199, Percent_Identity=24.1206030150754, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=202, Percent_Identity=23.7623762376238, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=37.8378378378378, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=32.0158102766798, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790281, Length=174, Percent_Identity=35.0574712643678, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788682, Length=242, Percent_Identity=32.6446280991736, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787660, Length=269, Percent_Identity=31.5985130111524, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=28.1954887218045, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI87082183, Length=271, Percent_Identity=26.9372693726937, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=38.0952380952381, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=36.2204724409449, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=34.8360655737705, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17558304, Length=210, Percent_Identity=31.4285714285714, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=25.390625, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=164, Percent_Identity=33.5365853658537, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=251, Percent_Identity=29.4820717131474, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=251, Percent_Identity=29.4820717131474, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=251, Percent_Identity=29.4820717131474, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=201, Percent_Identity=26.3681592039801, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=22.265625, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=205, Percent_Identity=23.9024390243902, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=41.3127413127413, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=38.7096774193548, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=38.7096774193548, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21357171, Length=178, Percent_Identity=32.5842696629214, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24650670, Length=178, Percent_Identity=33.1460674157303, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920382, Length=271, Percent_Identity=26.1992619926199, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=190, Percent_Identity=27.8947368421053, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571729, Length=190, Percent_Identity=27.8947368421053, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921018, Length=185, Percent_Identity=29.1891891891892, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=232, Percent_Identity=22.8448275862069, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583165, Length=139, Percent_Identity=33.0935251798561, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28663; Mature: 28663

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFSTVLYSTEKEIAVLLLNRPEKRNAISKELLSTLHKNILKAKKEKSIRSLVLSGVGP
CCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SFCAGADLKERVTMSPKEVKRFLEDLKNCFLELENFPYPTVAALDGDAFGGGLELALCCD
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
LILLKNDIRIGLTETRLGIIPGGGGTQRLSRRIGISKAKEMIFTGKTIDAQTALDFGIAN
HHHHHCCCEEEEEECEEEEEECCCCHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHH
SIWHDSSLPAAKMLAEEIASQCAPIALQLAKKAITEGYGQDIRKALITESKYYNNTLNTE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DRLEALKAFQEKRKPIFKGK
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNEFSTVLYSTEKEIAVLLLNRPEKRNAISKELLSTLHKNILKAKKEKSIRSLVLSGVGP
CCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SFCAGADLKERVTMSPKEVKRFLEDLKNCFLELENFPYPTVAALDGDAFGGGLELALCCD
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
LILLKNDIRIGLTETRLGIIPGGGGTQRLSRRIGISKAKEMIFTGKTIDAQTALDFGIAN
HHHHHCCCEEEEEECEEEEEECCCCHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHH
SIWHDSSLPAAKMLAEEIASQCAPIALQLAKKAITEGYGQDIRKALITESKYYNNTLNTE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DRLEALKAFQEKRKPIFKGK
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]