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The map label for this gene is speE [H]

Identifier: 45359147

GI number: 45359147

Start: 1533965

End: 1534807

Strand: Direct

Name: speE [H]

Synonym: MMP1584

Alternate gene names: 45359147

Gene position: 1533965-1534807 (Clockwise)

Preceding gene: 45359146

Following gene: 45359148

Centisome position: 92.34

GC content: 37.13

Gene sequence:

>843_bases
ATGAAATTCGATGCATGGTATACGGAACCACAAACTGAAAATTTAAGCCTTTCAACCAAATTAAAAGACATACTCTATGT
TGGGCAATCAGAATACCAAGAAATTCAAGTTCTTGACACTTACGAGTTTGGAAGAGTCTTGATTTTAGAAAACACGTACC
AAACTACTGAAAGAGATGAATTTATATACCACGAATTGATTTCACACCCTGCACTCTTTACGCACGGAAACCCTAAAAAA
GTTCTTGTCATCGGTGGAGGAGATGGCGGATCTGTCAGGGAAGTTTTAAAACATAAAAGCGTTGAAAAAATAGATTTTGT
TGAACTCGATGGACAGGTTGTAGAGGTTGCAAAAAAATTCCTTCCAACCCTAAGCTGCGAAATAGACAACGAGAAAGTAA
ACACGATAATTACGGATGGAATTAAATACGTTGCAGAAACTTCTGAAAAATACGATGTAATCCTCGTAGACTGCCCAGAC
CCAGTTGGCCCTGCTGCAGGATTATTCGAAAAAGAATTCTACAACAACCTCTACAAGTGCTTAAATGATGATGGAATCAT
GGTACAGCAGACAGAAAGCCCGCTTTTACATGAAAAATTGATAAATAAAATCAAAGGACACTTAAAAGATGCTGGCTTTT
CAACGATCCGCCCACTCGTATGTAGTATTCCAACATACCCAAGCGGATTCTGGAGCTTTACACTAGCTTCAAAGAAAAAC
GATCCATTAAATTCCGATGTTTCAGAAATTGAAAACAAATTAAAAGATATGACTACAAAATACTACGATCAGGACGTTCA
CAAGGGAGTATTTTTAGCAACACCTAGATACTTAAAAGAATAA

Upstream 100 bases:

>100_bases
CCGAGGATACAATCCCCGAAATTGAAAAATTCTTAAAACCTGAGAAAACAGAAGTTATGGATATCAAAAGAGGAATTATT
GATGACGGGGAGGTGAAAGA

Downstream 100 bases:

>100_bases
TTTCTAACTTTTTTATTATTTTTTGGTGATAATTCATGTATTTTGAAGATTATTCCAAGTTTCTCACAGCTTATGATACA
TTTAACGATGCTGATTTTGT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKFDAWYTEPQTENLSLSTKLKDILYVGQSEYQEIQVLDTYEFGRVLILENTYQTTERDEFIYHELISHPALFTHGNPKK
VLVIGGGDGGSVREVLKHKSVEKIDFVELDGQVVEVAKKFLPTLSCEIDNEKVNTIITDGIKYVAETSEKYDVILVDCPD
PVGPAAGLFEKEFYNNLYKCLNDDGIMVQQTESPLLHEKLINKIKGHLKDAGFSTIRPLVCSIPTYPSGFWSFTLASKKN
DPLNSDVSEIENKLKDMTTKYYDQDVHKGVFLATPRYLKE

Sequences:

>Translated_280_residues
MKFDAWYTEPQTENLSLSTKLKDILYVGQSEYQEIQVLDTYEFGRVLILENTYQTTERDEFIYHELISHPALFTHGNPKK
VLVIGGGDGGSVREVLKHKSVEKIDFVELDGQVVEVAKKFLPTLSCEIDNEKVNTIITDGIKYVAETSEKYDVILVDCPD
PVGPAAGLFEKEFYNNLYKCLNDDGIMVQQTESPLLHEKLINKIKGHLKDAGFSTIRPLVCSIPTYPSGFWSFTLASKKN
DPLNSDVSEIENKLKDMTTKYYDQDVHKGVFLATPRYLKE
>Mature_280_residues
MKFDAWYTEPQTENLSLSTKLKDILYVGQSEYQEIQVLDTYEFGRVLILENTYQTTERDEFIYHELISHPALFTHGNPKK
VLVIGGGDGGSVREVLKHKSVEKIDFVELDGQVVEVAKKFLPTLSCEIDNEKVNTIITDGIKYVAETSEKYDVILVDCPD
PVGPAAGLFEKEFYNNLYKCLNDDGIMVQQTESPLLHEKLINKIKGHLKDAGFSTIRPLVCSIPTYPSGFWSFTLASKKN
DPLNSDVSEIENKLKDMTTKYYDQDVHKGVFLATPRYLKE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=273, Percent_Identity=36.996336996337, Blast_Score=197, Evalue=9e-51,
Organism=Escherichia coli, GI1786312, Length=268, Percent_Identity=38.0597014925373, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25154106, Length=269, Percent_Identity=37.9182156133829, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI212645616, Length=223, Percent_Identity=42.6008968609865, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6325326, Length=281, Percent_Identity=35.9430604982206, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323175, Length=280, Percent_Identity=36.4285714285714, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24645443, Length=280, Percent_Identity=37.5, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=37.2093023255814, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31816; Mature: 31816

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDAWYTEPQTENLSLSTKLKDILYVGQSEYQEIQVLDTYEFGRVLILENTYQTTERDE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHH
FIYHELISHPALFTHGNPKKVLVIGGGDGGSVREVLKHKSVEKIDFVELDGQVVEVAKKF
HHHHHHHCCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
LPTLSCEIDNEKVNTIITDGIKYVAETSEKYDVILVDCPDPVGPAAGLFEKEFYNNLYKC
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LNDDGIMVQQTESPLLHEKLINKIKGHLKDAGFSTIRPLVCSIPTYPSGFWSFTLASKKN
HCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCC
DPLNSDVSEIENKLKDMTTKYYDQDVHKGVFLATPRYLKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCC
>Mature Secondary Structure
MKFDAWYTEPQTENLSLSTKLKDILYVGQSEYQEIQVLDTYEFGRVLILENTYQTTERDE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHH
FIYHELISHPALFTHGNPKKVLVIGGGDGGSVREVLKHKSVEKIDFVELDGQVVEVAKKF
HHHHHHHCCCEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
LPTLSCEIDNEKVNTIITDGIKYVAETSEKYDVILVDCPDPVGPAAGLFEKEFYNNLYKC
CCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LNDDGIMVQQTESPLLHEKLINKIKGHLKDAGFSTIRPLVCSIPTYPSGFWSFTLASKKN
HCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCC
DPLNSDVSEIENKLKDMTTKYYDQDVHKGVFLATPRYLKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]