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The map label for this gene is leuC [H]

Identifier: 45358712

GI number: 45358712

Start: 1137792

End: 1139066

Strand: Direct

Name: leuC [H]

Synonym: MMP1149

Alternate gene names: 45358712

Gene position: 1137792-1139066 (Clockwise)

Preceding gene: 45358708

Following gene: 45358717

Centisome position: 68.49

GC content: 38.98

Gene sequence:

>1275_bases
ATGGCAATGACCCTAGCTGAAAAAATTTTGGCAAAAGCTTCAGGTAACTCAGAGGTTTCACCTGGAGACATCGTTATGGC
TAAAGTAGAAACCGCGATGGTCCACGATATTACAGGCCCGCTTTCAGTAAATACATTAAAAAAAGAAGGTATTTCAAAAG
TTTGGGACAATGAAAAGATTGTGATTCCATTCGACCACCAGATACCTGCTGATAGCATCAACGCTGCTGAAAATCATATA
TTAATGAGAAACTTTGTAAAAGAACAAAATATCAAACACTTCTATGACATTAGAGAAGGAGTTTGCCATCAGGTATTACC
TGAAAAAGGTCACATAGTTCCAGGAACTGTTGTAGTTGGTGCAGACAGCCACACCTGTACATACGGTGCATTTGGTGCAT
TTGCAACAGGAATTGGAAGTACCGACATGGCTGCTGCATTTGCAACTGGTGAATTATGGTTTAAAGTTCCTGAAACACTT
TACTTCAATATAACTGGTGAGTTAAAACCAGAAGTAATGTCTAAAGATGTAATCTTGAGCATTATCGGAATGGTTGGAGC
TGACGGTGCAACATACAAAGCTGCACAGTTTGCCGGAAACACAGTAGATAACATGACTATTGCATCAAGAATGACAATGT
CAAACATGGCTATCGAAATGGGTGGAAAAGCAGGATTAATCGCACCAGACGATAAAACAATTAATTATGTAAAAAATGCA
ATGGAAGCTAACAACACTGCAAAACCATTCGAATTAGTTCTTGGAGACAAAAATGCAGAATTTGAAGAAAAATTCGAAAT
TGATGTAAGCAATCTTGAACCTGTTATGGCATGCCCGCACAACGTGGACAATGTAAAAGCTGTTAGAGAAGTTGCAGGAA
CCCCAATTGATCAGGTATTCATTGGTTCATGTACTAACGGAAGACTTGAAGATTTGAGAGCTGCTTTAAACGTTATTGAA
AAACACGGCGGAATTTCAAAAGATATCAGAGTTGTTGTAACCCCTGCATCAAGAAGCATCATGCTTGAAGCTATTGATGA
AGGATTAATTAAAAAATTCTACCAGTACGGCTGTGTTGTAACAAATCCATCATGTTCTGCATGTATGGGTGCATTATACG
GTCTTTTAGGTCCTGGAGAAGTTGGAATTGCAACTTCAAACAGAAACTTCAGAGGAAGAGAAGGTTCTTTAGAATCAGAC
GTGTACCTTGCATCGCCAATTACTGCTGCAGCATGTGCTGTTAAAGGAGAAATTGTTGACCCAAGAGATCTCTAA

Upstream 100 bases:

>100_bases
CTAATTCTAATTCGTAGAAATAAATAAATAGTTCTGCTATATAATACTTTCCAAGTAAGTACGTCTTATTTGTCATATGG
TGCTATATGGAGGTATAAAC

Downstream 100 bases:

>100_bases
TCTAATTAAAACCTTATTTTTTTACTTTTTTTAAATTTTAAAGTTAAGAAGAAAAAAAGAATTTTTTATTGGTTATTTAA
TCTTGTTTTTTAAAATCTTC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAMTLAEKILAKASGNSEVSPGDIVMAKVETAMVHDITGPLSVNTLKKEGISKVWDNEKIVIPFDHQIPADSINAAENHI
LMRNFVKEQNIKHFYDIREGVCHQVLPEKGHIVPGTVVVGADSHTCTYGAFGAFATGIGSTDMAAAFATGELWFKVPETL
YFNITGELKPEVMSKDVILSIIGMVGADGATYKAAQFAGNTVDNMTIASRMTMSNMAIEMGGKAGLIAPDDKTINYVKNA
MEANNTAKPFELVLGDKNAEFEEKFEIDVSNLEPVMACPHNVDNVKAVREVAGTPIDQVFIGSCTNGRLEDLRAALNVIE
KHGGISKDIRVVVTPASRSIMLEAIDEGLIKKFYQYGCVVTNPSCSACMGALYGLLGPGEVGIATSNRNFRGREGSLESD
VYLASPITAAACAVKGEIVDPRDL

Sequences:

>Translated_424_residues
MAMTLAEKILAKASGNSEVSPGDIVMAKVETAMVHDITGPLSVNTLKKEGISKVWDNEKIVIPFDHQIPADSINAAENHI
LMRNFVKEQNIKHFYDIREGVCHQVLPEKGHIVPGTVVVGADSHTCTYGAFGAFATGIGSTDMAAAFATGELWFKVPETL
YFNITGELKPEVMSKDVILSIIGMVGADGATYKAAQFAGNTVDNMTIASRMTMSNMAIEMGGKAGLIAPDDKTINYVKNA
MEANNTAKPFELVLGDKNAEFEEKFEIDVSNLEPVMACPHNVDNVKAVREVAGTPIDQVFIGSCTNGRLEDLRAALNVIE
KHGGISKDIRVVVTPASRSIMLEAIDEGLIKKFYQYGCVVTNPSCSACMGALYGLLGPGEVGIATSNRNFRGREGSLESD
VYLASPITAAACAVKGEIVDPRDL
>Mature_423_residues
AMTLAEKILAKASGNSEVSPGDIVMAKVETAMVHDITGPLSVNTLKKEGISKVWDNEKIVIPFDHQIPADSINAAENHIL
MRNFVKEQNIKHFYDIREGVCHQVLPEKGHIVPGTVVVGADSHTCTYGAFGAFATGIGSTDMAAAFATGELWFKVPETLY
FNITGELKPEVMSKDVILSIIGMVGADGATYKAAQFAGNTVDNMTIASRMTMSNMAIEMGGKAGLIAPDDKTINYVKNAM
EANNTAKPFELVLGDKNAEFEEKFEIDVSNLEPVMACPHNVDNVKAVREVAGTPIDQVFIGSCTNGRLEDLRAALNVIEK
HGGISKDIRVVVTPASRSIMLEAIDEGLIKKFYQYGCVVTNPSCSACMGALYGLLGPGEVGIATSNRNFRGREGSLESDV
YLASPITAAACAVKGEIVDPRDL

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=26.1744966442953, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI8659555, Length=442, Percent_Identity=21.9457013574661, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=23.3766233766234, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786259, Length=473, Percent_Identity=33.4038054968288, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI2367097, Length=437, Percent_Identity=24.4851258581236, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787531, Length=390, Percent_Identity=23.3333333333333, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081781, Length=316, Percent_Identity=22.7848101265823, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32564738, Length=355, Percent_Identity=25.3521126760563, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25149337, Length=355, Percent_Identity=25.6338028169014, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17568399, Length=394, Percent_Identity=20.8121827411168, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=446, Percent_Identity=32.5112107623318, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321429, Length=451, Percent_Identity=33.4811529933481, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323335, Length=345, Percent_Identity=30.1449275362319, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=348, Percent_Identity=27.8735632183908, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=25.4464285714286, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=25.4464285714286, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=26.4631043256997, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=446, Percent_Identity=24.2152466367713, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=23.2432432432432, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24645686, Length=374, Percent_Identity=22.9946524064171, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 45409; Mature: 45277

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMTLAEKILAKASGNSEVSPGDIVMAKVETAMVHDITGPLSVNTLKKEGISKVWDNEKI
CCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCEE
VIPFDHQIPADSINAAENHILMRNFVKEQNIKHFYDIREGVCHQVLPEKGHIVPGTVVVG
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
ADSHTCTYGAFGAFATGIGSTDMAAAFATGELWFKVPETLYFNITGELKPEVMSKDVILS
CCCCCEECCCHHHHHHCCCCCHHHHHEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHH
IIGMVGADGATYKAAQFAGNTVDNMTIASRMTMSNMAIEMGGKAGLIAPDDKTINYVKNA
HHHHHCCCCCCEEHHHHCCCCCCCCHHHHHHHHHHHEEEECCCCCEECCCCCHHHHHHHH
MEANNTAKPFELVLGDKNAEFEEKFEIDVSNLEPVMACPHNVDNVKAVREVAGTPIDQVF
HHCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCHHHEE
IGSCTNGRLEDLRAALNVIEKHGGISKDIRVVVTPASRSIMLEAIDEGLIKKFYQYGCVV
EECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEE
TNPSCSACMGALYGLLGPGEVGIATSNRNFRGREGSLESDVYLASPITAAACAVKGEIVD
ECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCC
PRDL
CCCC
>Mature Secondary Structure 
AMTLAEKILAKASGNSEVSPGDIVMAKVETAMVHDITGPLSVNTLKKEGISKVWDNEKI
CHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCEE
VIPFDHQIPADSINAAENHILMRNFVKEQNIKHFYDIREGVCHQVLPEKGHIVPGTVVVG
EEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
ADSHTCTYGAFGAFATGIGSTDMAAAFATGELWFKVPETLYFNITGELKPEVMSKDVILS
CCCCCEECCCHHHHHHCCCCCHHHHHEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHH
IIGMVGADGATYKAAQFAGNTVDNMTIASRMTMSNMAIEMGGKAGLIAPDDKTINYVKNA
HHHHHCCCCCCEEHHHHCCCCCCCCHHHHHHHHHHHEEEECCCCCEECCCCCHHHHHHHH
MEANNTAKPFELVLGDKNAEFEEKFEIDVSNLEPVMACPHNVDNVKAVREVAGTPIDQVF
HHCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCHHHEE
IGSCTNGRLEDLRAALNVIEKHGGISKDIRVVVTPASRSIMLEAIDEGLIKKFYQYGCVV
EECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEE
TNPSCSACMGALYGLLGPGEVGIATSNRNFRGREGSLESDVYLASPITAAACAVKGEIVD
ECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCC
PRDL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]