Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 45357959

GI number: 45357959

Start: 393105

End: 394397

Strand: Direct

Name: eno [H]

Synonym: MMP0396

Alternate gene names: 45357959

Gene position: 393105-394397 (Clockwise)

Preceding gene: 45357950

Following gene: 45357960

Centisome position: 23.66

GC content: 37.74

Gene sequence:

>1293_bases
TTGTTAAGAAACATGGACGATTCATTTGATATATATGAAATAAAAGCAAGACAGGTACTTGATTCACGGGGAAACCCAAC
TGTGGAAGCTGAAGTGTTAACTGCAGGTGGTGGATACGGTCATACAATTGTTCCAAGCGGTGCTTCAACAGGAACTTTCG
AAGCAGTTGAATTAAGAGATAAAAAAGAAAAATATGGCGGAAAAAGCGTATTAAATGCAGTTTCAAACGTAAATGATATT
ATTGCACAGGAATTAATCGGTGAAGATGCAAGAAACCAGAGATTAATTGATCAAATAATGTTAAACTTGGATGGAACTGA
AAATAAAGGAAATTTGGGCGCAAATGCAATTTTAGCAGTTTCACTTGCAGTTGCAAAAGCAGCGGCAGACACTGCATCAC
TTCCACTTTATAAATACATCGGTGGCTCTAACGCATATGTTATGCCTGCCCCAATGATGAACGTGTTAAACGGTGGCCAG
CACGCAGGAAATGCACTTGATTTTCAGGAATTCATGATAATGCCAGTTGGCGCGGATTCATTTGCAGAAGCTGTAAGAAT
GTGTTCTGAAACATACCAATCTTTGAAAAAAGTAGTTGCTGAAAAATACGGAAAAGATGCTGTAAATGTAGGGGATGAAG
GTGGATTTGCACCCCCTGTAAAAACAATCGACGAAGCTCTCGCAGTACTTTTAGAAGGGGTTAAAAAAGCAGGTTATGAA
GACGAAATCGTCTTTACAATCGATTCTGCCGCAAGTGAGTTTTACGATGAAAAATCCGGTTCATATATGATTGCAGGAGA
AAAAGTTTCAACCGATAAATTAATCGATATTTACAAAGAAATGGTTGAACAATATCCAATTGTTTCAATTGAGGATCCAT
TGTTTGAAGAAGATTTCGAAGGATTTAAAACTGCAACAAAAGAGTTAAAAGGAATTCAGATTGTCGGTGACGATTTGTTT
GTTACAAACACGAAAAGACTCAAAAAAGGAATTGAAATGGGCGCTGCAAATTCACTTCTCTTGAAAGTAAATCAGATTGG
AACCCTTTCAGAATCAATCGATGCTGCAAACATGGCATTTAGAAATGGATACAGTTTGGTTGTTTCACACAGAAGTGGAG
AATCAGAGGACTCAACAATTGCAGATTTAGCCGTTGCACTAAATGCAGGCCAGATAAAAACCGGTGCTCCTGCAAGGGGA
GAAAGAACTGCAAAATACAACCAGTTAATGAGAATCGAAGAAGAACTCCAGATTTCAAAATATGCTGGAAAAGATTTCAA
AATTCCATTTTAA

Upstream 100 bases:

>100_bases
AAAACTATATATTTCTTAAACCAATATCATTGATGTAGTTTTTAGCGGCATTCATCTTTTTTAAACTTTAATAATTTGGA
AATTTACCAGAGGTGAGACT

Downstream 100 bases:

>100_bases
TTCTATTTAATTCTATTTTTCAAATTTTTTTTATTCAATTTTATTTAAAATATGTATTTTCCCAAGATTTTTCAAATTTT
TAATTTAAAGTTATATATTA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MLRNMDDSFDIYEIKARQVLDSRGNPTVEAEVLTAGGGYGHTIVPSGASTGTFEAVELRDKKEKYGGKSVLNAVSNVNDI
IAQELIGEDARNQRLIDQIMLNLDGTENKGNLGANAILAVSLAVAKAAADTASLPLYKYIGGSNAYVMPAPMMNVLNGGQ
HAGNALDFQEFMIMPVGADSFAEAVRMCSETYQSLKKVVAEKYGKDAVNVGDEGGFAPPVKTIDEALAVLLEGVKKAGYE
DEIVFTIDSAASEFYDEKSGSYMIAGEKVSTDKLIDIYKEMVEQYPIVSIEDPLFEEDFEGFKTATKELKGIQIVGDDLF
VTNTKRLKKGIEMGAANSLLLKVNQIGTLSESIDAANMAFRNGYSLVVSHRSGESEDSTIADLAVALNAGQIKTGAPARG
ERTAKYNQLMRIEEELQISKYAGKDFKIPF

Sequences:

>Translated_430_residues
MLRNMDDSFDIYEIKARQVLDSRGNPTVEAEVLTAGGGYGHTIVPSGASTGTFEAVELRDKKEKYGGKSVLNAVSNVNDI
IAQELIGEDARNQRLIDQIMLNLDGTENKGNLGANAILAVSLAVAKAAADTASLPLYKYIGGSNAYVMPAPMMNVLNGGQ
HAGNALDFQEFMIMPVGADSFAEAVRMCSETYQSLKKVVAEKYGKDAVNVGDEGGFAPPVKTIDEALAVLLEGVKKAGYE
DEIVFTIDSAASEFYDEKSGSYMIAGEKVSTDKLIDIYKEMVEQYPIVSIEDPLFEEDFEGFKTATKELKGIQIVGDDLF
VTNTKRLKKGIEMGAANSLLLKVNQIGTLSESIDAANMAFRNGYSLVVSHRSGESEDSTIADLAVALNAGQIKTGAPARG
ERTAKYNQLMRIEEELQISKYAGKDFKIPF
>Mature_430_residues
MLRNMDDSFDIYEIKARQVLDSRGNPTVEAEVLTAGGGYGHTIVPSGASTGTFEAVELRDKKEKYGGKSVLNAVSNVNDI
IAQELIGEDARNQRLIDQIMLNLDGTENKGNLGANAILAVSLAVAKAAADTASLPLYKYIGGSNAYVMPAPMMNVLNGGQ
HAGNALDFQEFMIMPVGADSFAEAVRMCSETYQSLKKVVAEKYGKDAVNVGDEGGFAPPVKTIDEALAVLLEGVKKAGYE
DEIVFTIDSAASEFYDEKSGSYMIAGEKVSTDKLIDIYKEMVEQYPIVSIEDPLFEEDFEGFKTATKELKGIQIVGDDLF
VTNTKRLKKGIEMGAANSLLLKVNQIGTLSESIDAANMAFRNGYSLVVSHRSGESEDSTIADLAVALNAGQIKTGAPARG
ERTAKYNQLMRIEEELQISKYAGKDFKIPF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=53.1177829099307, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=51.3824884792627, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=52.1939953810624, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=52.1939953810624, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=48.491879350348, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=56.8345323741007, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=53.3180778032037, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=53.3180778032037, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=200, Percent_Identity=48.5, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=52.4027459954233, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=51.1520737327189, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=51.1520737327189, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=51.1520737327189, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=51.7162471395881, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=53.2258064516129, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=53.2258064516129, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=53.2258064516129, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=53.2258064516129, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=53.2258064516129, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=53.2258064516129, Blast_Score=411, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46444; Mature: 46444

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNMDDSFDIYEIKARQVLDSRGNPTVEAEVLTAGGGYGHTIVPSGASTGTFEAVELRD
CCCCCCCCCCEEEHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCEEEHHHHH
KKEKYGGKSVLNAVSNVNDIIAQELIGEDARNQRLIDQIMLNLDGTENKGNLGANAILAV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
SLAVAKAAADTASLPLYKYIGGSNAYVMPAPMMNVLNGGQHAGNALDFQEFMIMPVGADS
HHHHHHHHCCCCCCCHHEEECCCCCEEECHHHHHHHCCCCCCCCCCCCCCEEEEECCCHH
FAEAVRMCSETYQSLKKVVAEKYGKDAVNVGDEGGFAPPVKTIDEALAVLLEGVKKAGYE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DEIVFTIDSAASEFYDEKSGSYMIAGEKVSTDKLIDIYKEMVEQYPIVSIEDPLFEEDFE
CEEEEEECHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
GFKTATKELKGIQIVGDDLFVTNTKRLKKGIEMGAANSLLLKVNQIGTLSESIDAANMAF
HHHHHHHHHCCEEEECCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
RNGYSLVVSHRSGESEDSTIADLAVALNAGQIKTGAPARGERTAKYNQLMRIEEELQISK
HCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YAGKDFKIPF
HCCCCCCCCC
>Mature Secondary Structure
MLRNMDDSFDIYEIKARQVLDSRGNPTVEAEVLTAGGGYGHTIVPSGASTGTFEAVELRD
CCCCCCCCCCEEEHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCEEEHHHHH
KKEKYGGKSVLNAVSNVNDIIAQELIGEDARNQRLIDQIMLNLDGTENKGNLGANAILAV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
SLAVAKAAADTASLPLYKYIGGSNAYVMPAPMMNVLNGGQHAGNALDFQEFMIMPVGADS
HHHHHHHHCCCCCCCHHEEECCCCCEEECHHHHHHHCCCCCCCCCCCCCCEEEEECCCHH
FAEAVRMCSETYQSLKKVVAEKYGKDAVNVGDEGGFAPPVKTIDEALAVLLEGVKKAGYE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DEIVFTIDSAASEFYDEKSGSYMIAGEKVSTDKLIDIYKEMVEQYPIVSIEDPLFEEDFE
CEEEEEECHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHH
GFKTATKELKGIQIVGDDLFVTNTKRLKKGIEMGAANSLLLKVNQIGTLSESIDAANMAF
HHHHHHHHHCCEEEECCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
RNGYSLVVSHRSGESEDSTIADLAVALNAGQIKTGAPARGERTAKYNQLMRIEEELQISK
HCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YAGKDFKIPF
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA