Click here to switch to the map view.

The map label for this gene is lasT [C]

Identifier: 45357897

GI number: 45357897

Start: 331555

End: 332259

Strand: Direct

Name: lasT [C]

Synonym: MMP0334

Alternate gene names: 45357897

Gene position: 331555-332259 (Clockwise)

Preceding gene: 45357896

Following gene: 45357898

Centisome position: 19.96

GC content: 34.18

Gene sequence:

>705_bases
GTGAAAACAGTAGTTATATTGGTTAATCCAAAATATGGTGGGAATTTAGGGGCTATTGCAAGAAATATGATGAATTTTGA
TGTGGAAGAATTAAGAATAGTTGGAACGCCTGAAATTTTAGATGATGAAGCATATATTCGGGCAGTTCACGCGAAAAACA
TACTAAACAATGCTAAATTTTATTCAAAACTTGAAGACGCAATTTCAGATATTGATTTTACCGTGGCAACAACGGGGGCA
GTTTGCGGTGATAGAAACGTAAACCGGGTTCCAATTACTCCTCGAGAACTTGCTAAAAAACATTCGAGCATATCTGGAAC
ATTAGGAATAGTTTTTGGGCGGGAAGATGATGGCCTAAAAAATGAAGATGTAGAACTCTGCGATATTTTAGTTTCCGTTC
CAACTTCAAACAAATACCCAATATTGAATCTTTCTCACGCCGTTTCAGTAATATTGTACGAAATTTACTTTGAATCATTT
TCAAAAGATATTCCTTACGATTTAAACATGAAAAACGCATCAGTTGATGAAAAAAATGCACTTCTTAGATTTTTCAACGA
GTTTGTAGATCGTTCAAAAGATGTGCCAGACTATAGAAAAGACATATGCAAGACAATTTTTAAAAGAATTGTTGGCAGGG
CGTTTATTTCGGGAAAAGAAGCAAATTCCTTAATATGTGTTTTTAAAGATAAATGCCCGAAATAA

Upstream 100 bases:

>100_bases
CAAAGATTATATTTTTAACGATAGTTAATATTTAATCAGAATCTATAATAATTATAAAATTGAATTGGAATAAGAAATTA
AAGTGAAAACTTGTGATAAC

Downstream 100 bases:

>100_bases
TATGGAAAGTTTTTTAAACCGTGCAAGGAATAATAATATTATCAATAGTATGGCGGAAAAGGTGAGGGGAATGAGTATAT
CTGGTGCACAGGGAAAGTCT

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKTVVILVNPKYGGNLGAIARNMMNFDVEELRIVGTPEILDDEAYIRAVHAKNILNNAKFYSKLEDAISDIDFTVATTGA
VCGDRNVNRVPITPRELAKKHSSISGTLGIVFGREDDGLKNEDVELCDILVSVPTSNKYPILNLSHAVSVILYEIYFESF
SKDIPYDLNMKNASVDEKNALLRFFNEFVDRSKDVPDYRKDICKTIFKRIVGRAFISGKEANSLICVFKDKCPK

Sequences:

>Translated_234_residues
MKTVVILVNPKYGGNLGAIARNMMNFDVEELRIVGTPEILDDEAYIRAVHAKNILNNAKFYSKLEDAISDIDFTVATTGA
VCGDRNVNRVPITPRELAKKHSSISGTLGIVFGREDDGLKNEDVELCDILVSVPTSNKYPILNLSHAVSVILYEIYFESF
SKDIPYDLNMKNASVDEKNALLRFFNEFVDRSKDVPDYRKDICKTIFKRIVGRAFISGKEANSLICVFKDKCPK
>Mature_234_residues
MKTVVILVNPKYGGNLGAIARNMMNFDVEELRIVGTPEILDDEAYIRAVHAKNILNNAKFYSKLEDAISDIDFTVATTGA
VCGDRNVNRVPITPRELAKKHSSISGTLGIVFGREDDGLKNEDVELCDILVSVPTSNKYPILNLSHAVSVILYEIYFESF
SKDIPYDLNMKNASVDEKNALLRFFNEFVDRSKDVPDYRKDICKTIFKRIVGRAFISGKEANSLICVFKDKCPK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=155, Percent_Identity=36.7741935483871, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788881, Length=176, Percent_Identity=27.8409090909091, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26249; Mature: 26249

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVVILVNPKYGGNLGAIARNMMNFDVEELRIVGTPEILDDEAYIRAVHAKNILNNAKF
CCEEEEEECCCCCCCHHHHHHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
YSKLEDAISDIDFTVATTGAVCGDRNVNRVPITPRELAKKHSSISGTLGIVFGREDDGLK
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
NEDVELCDILVSVPTSNKYPILNLSHAVSVILYEIYFESFSKDIPYDLNMKNASVDEKNA
CCCHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
LLRFFNEFVDRSKDVPDYRKDICKTIFKRIVGRAFISGKEANSLICVFKDKCPK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKTVVILVNPKYGGNLGAIARNMMNFDVEELRIVGTPEILDDEAYIRAVHAKNILNNAKF
CCEEEEEECCCCCCCHHHHHHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
YSKLEDAISDIDFTVATTGAVCGDRNVNRVPITPRELAKKHSSISGTLGIVFGREDDGLK
HHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCC
NEDVELCDILVSVPTSNKYPILNLSHAVSVILYEIYFESFSKDIPYDLNMKNASVDEKNA
CCCHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
LLRFFNEFVDRSKDVPDYRKDICKTIFKRIVGRAFISGKEANSLICVFKDKCPK
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]