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The map label for this gene is rfcB

Identifier: 45357885

GI number: 45357885

Start: 318173

End: 319633

Strand: Direct

Name: rfcB

Synonym: MMP0322

Alternate gene names: 45357885

Gene position: 318173-319633 (Clockwise)

Preceding gene: 45357884

Following gene: 45357889

Centisome position: 19.15

GC content: 35.59

Gene sequence:

>1461_bases
ATGGAAGAGTGGGTTGAAAAATACAGGCCAAAGTCATTAAATGATGTTGCAGGGCACAATAAAACTAAACAAGCGCTTAT
TGAATGGATTGAATCTATCATAGGTGGCCAAAATCAAAAACCAATACTCTTGGCAGGGCCTCCAGGATCGGGTAAAACCA
CATTAGCTTACGCGATTGCAAACGATTATGCATTTGATGTAATCGAACTTAATGCAAGTGACAAGCGAAATAAGGATGTA
ATTTCACAAGTTGTGGGAACTGCGGCAACTTCAAAATCTCTTACTGGAAGAAGGACGTTAATCGTTTTAGACGAGGTTGA
CGGGCTCTCTGGAAATGATGATAGGGGAGGGGTAGCTGAAATAATAAAAGTTTTAAAAACAGCAGAAAACCCTGTAATTT
TAACTGCAAACGATGTATACAAACCTGCTTTAATGACGCTTAGAAATTCTGTAAATCTGATAAATGTCGGTTCAGTTCAC
ACAAACTCGATTCCGCCGGTTTTAAGAAGAATTGCACTAAAAGAAGGTTTTGAAATTGATGAAAAAATAATTAAAATGAT
TGCAAGTCATTCTGGCGGAGATTTAAGGGCTGCAATAAATGATTTACAATCACTTGCAACAGGTGGCTCTATTGAAATCG
AAGATGCAAAAGAACTTCCCGATAGGGACAGTGAAAAGAGCATTTTCGATGCAATGAGAATAATAATGAAAACAACCCAC
TACGACATTGCAACAAGTGCTACAAGAGATGTAAAAGAAGATATTGGAACCATTGAAGAGTGGATTTCTGAAAATCTTCC
AAAAGAATATTTAAAATACAAGGATCTTGCAGAAGGGTATGATTACCTTTCAAAATCAGATGTATTTTTGGGAAGGGTTT
ACCGAAGACAGTATTTTGGACTTTGGAGATATGCTTCAGCTTTGATGACTGCAGGGACTGCTTTAGCAAAAGAAGAAAAG
TATCGAGGATTTACCAGATATGGGCCACCCGCCATATTTACAAAATTAAGCAGGACTAAGGGTAGCAGACAGAAAATGAA
AGATATTTTGAAAAAAATAGCTTTAAAGACCCATACTTCAACAAAAAGAGCAAGAAACACTGTAGATTACTTGACTGTAA
TTTTTGAATCAAATGCGGAAGTTTCTGCAGAACTTGTGGAATATTATGAACTTACAAAGGATGAAATAGAGTTTTTAACT
AACAAAACAATTACAAAAAAGATACTTTCAGTAATTGCAGGTAAAAAACCAAAAGTTAAAAAAGAAACTCCAAAGAAAAC
GGAAAAACCAAAAGAAGTAATGCCAATTATTCCAAAACGCCCAAGAATTTCGGAACCTCCAAAAGAACCTTTAAAAGAAG
TAATCGAAGAAACCCTCGAAAAATCTGTTGAAAAAGCAGATACAAAAGAAGAAAAGAAAAAAGATCCAAAGAAACAGGCG
ACTTTGGACAGTTTCTTTTAA

Upstream 100 bases:

>100_bases
AAAAGAAGAAATTAATGATGAAATAACTAGATTAATGAAAATTATTAAAAAAGTCGGCAGTAAAAATCATATCGAACTTG
CAAAAAAGTTAGGTGTTTAA

Downstream 100 bases:

>100_bases
AAATTTAAGTTAATTCTTTTTTAGTATTTTAAAGTCAATTACAAATACGTATTCTCTTGGGGAATAGGATTTTACGATTC
GTTTGTCCAAAATTTCACAT

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MEEWVEKYRPKSLNDVAGHNKTKQALIEWIESIIGGQNQKPILLAGPPGSGKTTLAYAIANDYAFDVIELNASDKRNKDV
ISQVVGTAATSKSLTGRRTLIVLDEVDGLSGNDDRGGVAEIIKVLKTAENPVILTANDVYKPALMTLRNSVNLINVGSVH
TNSIPPVLRRIALKEGFEIDEKIIKMIASHSGGDLRAAINDLQSLATGGSIEIEDAKELPDRDSEKSIFDAMRIIMKTTH
YDIATSATRDVKEDIGTIEEWISENLPKEYLKYKDLAEGYDYLSKSDVFLGRVYRRQYFGLWRYASALMTAGTALAKEEK
YRGFTRYGPPAIFTKLSRTKGSRQKMKDILKKIALKTHTSTKRARNTVDYLTVIFESNAEVSAELVEYYELTKDEIEFLT
NKTITKKILSVIAGKKPKVKKETPKKTEKPKEVMPIIPKRPRISEPPKEPLKEVIEETLEKSVEKADTKEEKKKDPKKQA
TLDSFF

Sequences:

>Translated_486_residues
MEEWVEKYRPKSLNDVAGHNKTKQALIEWIESIIGGQNQKPILLAGPPGSGKTTLAYAIANDYAFDVIELNASDKRNKDV
ISQVVGTAATSKSLTGRRTLIVLDEVDGLSGNDDRGGVAEIIKVLKTAENPVILTANDVYKPALMTLRNSVNLINVGSVH
TNSIPPVLRRIALKEGFEIDEKIIKMIASHSGGDLRAAINDLQSLATGGSIEIEDAKELPDRDSEKSIFDAMRIIMKTTH
YDIATSATRDVKEDIGTIEEWISENLPKEYLKYKDLAEGYDYLSKSDVFLGRVYRRQYFGLWRYASALMTAGTALAKEEK
YRGFTRYGPPAIFTKLSRTKGSRQKMKDILKKIALKTHTSTKRARNTVDYLTVIFESNAEVSAELVEYYELTKDEIEFLT
NKTITKKILSVIAGKKPKVKKETPKKTEKPKEVMPIIPKRPRISEPPKEPLKEVIEETLEKSVEKADTKEEKKKDPKKQA
TLDSFF
>Mature_486_residues
MEEWVEKYRPKSLNDVAGHNKTKQALIEWIESIIGGQNQKPILLAGPPGSGKTTLAYAIANDYAFDVIELNASDKRNKDV
ISQVVGTAATSKSLTGRRTLIVLDEVDGLSGNDDRGGVAEIIKVLKTAENPVILTANDVYKPALMTLRNSVNLINVGSVH
TNSIPPVLRRIALKEGFEIDEKIIKMIASHSGGDLRAAINDLQSLATGGSIEIEDAKELPDRDSEKSIFDAMRIIMKTTH
YDIATSATRDVKEDIGTIEEWISENLPKEYLKYKDLAEGYDYLSKSDVFLGRVYRRQYFGLWRYASALMTAGTALAKEEK
YRGFTRYGPPAIFTKLSRTKGSRQKMKDILKKIALKTHTSTKRARNTVDYLTVIFESNAEVSAELVEYYELTKDEIEFLT
NKTITKKILSVIAGKKPKVKKETPKKTEKPKEVMPIIPKRPRISEPPKEPLKEVIEETLEKSVEKADTKEEKKKDPKKQA
TLDSFF

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=431, Percent_Identity=25.2900232018561, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI6677723, Length=305, Percent_Identity=26.2295081967213, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI194306571, Length=305, Percent_Identity=25.9016393442623, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI194306567, Length=305, Percent_Identity=25.9016393442623, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI31563534, Length=324, Percent_Identity=26.8518518518519, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4506491, Length=224, Percent_Identity=29.4642857142857, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI31881687, Length=224, Percent_Identity=29.4642857142857, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17563226, Length=466, Percent_Identity=24.2489270386266, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17541988, Length=300, Percent_Identity=28, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17541368, Length=297, Percent_Identity=25.2525252525253, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17554730, Length=224, Percent_Identity=29.4642857142857, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71986063, Length=221, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324791, Length=225, Percent_Identity=30.2222222222222, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323724, Length=303, Percent_Identity=25.4125412541254, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324039, Length=215, Percent_Identity=30.6976744186047, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324478, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322528, Length=235, Percent_Identity=27.2340425531915, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737765, Length=237, Percent_Identity=30.8016877637131, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI62472391, Length=257, Percent_Identity=29.1828793774319, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574716, Length=339, Percent_Identity=26.2536873156342, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19921076, Length=208, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17647857, Length=228, Percent_Identity=26.7543859649123, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI18859927, Length=217, Percent_Identity=28.110599078341, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_METMP (Q6M0E9)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_987442.1
- ProteinModelPortal:   Q6M0E9
- SMR:   Q6M0E9
- GeneID:   2761723
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0322
- NMPDR:   fig|267377.1.peg.322
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- ProtClustDB:   PRK04195
- BioCyc:   MMAR267377:MMP0322-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: 3.4.24.- [C]

Molecular weight: Translated: 54502; Mature: 54502

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEWVEKYRPKSLNDVAGHNKTKQALIEWIESIIGGQNQKPILLAGPPGSGKTTLAYAIA
CCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEE
NDYAFDVIELNASDKRNKDVISQVVGTAATSKSLTGRRTLIVLDEVDGLSGNDDRGGVAE
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
IIKVLKTAENPVILTANDVYKPALMTLRNSVNLINVGSVHTNSIPPVLRRIALKEGFEID
HHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHH
EKIIKMIASHSGGDLRAAINDLQSLATGGSIEIEDAKELPDRDSEKSIFDAMRIIMKTTH
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCCCCCHHHHHHHHHHHHHHHC
YDIATSATRDVKEDIGTIEEWISENLPKEYLKYKDLAEGYDYLSKSDVFLGRVYRRQYFG
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LWRYASALMTAGTALAKEEKYRGFTRYGPPAIFTKLSRTKGSRQKMKDILKKIALKTHTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCH
TKRARNTVDYLTVIFESNAEVSAELVEYYELTKDEIEFLTNKTITKKILSVIAGKKPKVK
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCC
KETPKKTEKPKEVMPIIPKRPRISEPPKEPLKEVIEETLEKSVEKADTKEEKKKDPKKQA
CCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHH
TLDSFF
HHHHCC
>Mature Secondary Structure
MEEWVEKYRPKSLNDVAGHNKTKQALIEWIESIIGGQNQKPILLAGPPGSGKTTLAYAIA
CCHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEE
NDYAFDVIELNASDKRNKDVISQVVGTAATSKSLTGRRTLIVLDEVDGLSGNDDRGGVAE
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHH
IIKVLKTAENPVILTANDVYKPALMTLRNSVNLINVGSVHTNSIPPVLRRIALKEGFEID
HHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHH
EKIIKMIASHSGGDLRAAINDLQSLATGGSIEIEDAKELPDRDSEKSIFDAMRIIMKTTH
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCCCCCHHHHHHHHHHHHHHHC
YDIATSATRDVKEDIGTIEEWISENLPKEYLKYKDLAEGYDYLSKSDVFLGRVYRRQYFG
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LWRYASALMTAGTALAKEEKYRGFTRYGPPAIFTKLSRTKGSRQKMKDILKKIALKTHTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCH
TKRARNTVDYLTVIFESNAEVSAELVEYYELTKDEIEFLTNKTITKKILSVIAGKKPKVK
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCC
KETPKKTEKPKEVMPIIPKRPRISEPPKEPLKEVIEETLEKSVEKADTKEEKKKDPKKQA
CCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHH
TLDSFF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA