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The map label for this gene is thrB [H]

Identifier: 45357858

GI number: 45357858

Start: 295253

End: 296158

Strand: Direct

Name: thrB [H]

Synonym: MMP0295

Alternate gene names: 45357858

Gene position: 295253-296158 (Clockwise)

Preceding gene: 45357857

Following gene: 45357861

Centisome position: 17.77

GC content: 37.2

Gene sequence:

>906_bases
ATGAAAAAAGTAAAGGTATGTTCTCCGGGAACTTCTGCAAATCTCGGTCCAGGATACGATATTTTTGGGCTTGCCCTTTC
AAATCCTTATGATATTGTAGAGGTTGAAAAAACAGAAAATGGTATCACGATATCTGTTGAAGGGGAAAAAGCAGAAGAAA
TACCTACAAATGTGGACGAAAATACTGCAGGCGTAGTTGCTAAAAAGATGATTGAAGATTTTAAAATCGAATCTGGAATT
CATATTCATATAATTAAAGGAATAAAACCCGGAAGCGGTCTTGGAAGCAGTTCTGCCTCATGTGCAGGAATTGCTTTTGC
GTTAAATGAACTTTTTGAACTAAAATTATCCAAATTAGAACTAGTTAAATATTCTTCACTTGGGGAAGCAGTTGCAGCAG
GTGCTCCTCACGCAGATAACGTAGCTCCAGCAATTTTTGGTGGATTTACTCTTACAACAAGTTATGAACCTTTAGAGGTA
CTTCACATCCCTGTGGACCTTGAAGTTTTGGTTGCACTTCCAAATATTCAGGTAAGTACAAAAACCGCAAGAGAAATACT
TCCAAAAGAAATCCCGATAAAAGATATGGTAAACAATGTCGGAAAAGCTGCAGGGATGGTTTATGCATTATATAACAACG
ATCTAGATCTTTTTGGAAGATACATGTCAAAAGACTGCGTTGTTGAGCCATGCAGGGCCAATTTAATTGACGGATATACG
GAAGTTAAAGAAAAAGTAAAAGACATGGTCTATGGTATAACGATTAGTGGTTCGGGACCTGCAATTATTACAATACCTAA
AAAAGAACACGTAATTGATATCGAAAATATATTTAAGGAAGTCTGGAACTGCCCAGTTTACTACACGAAAGTGGGGCCCG
GATGTTACGTTGAAGAAATTGAATAA

Upstream 100 bases:

>100_bases
TACGTTCAAAAGAGTGAATTCTTAAGTAGCATTTTTATATCTATTTTTACTTATTGTTTATTGAGCGATAAACAATATTC
AGGATTATTTTGGTGGTATT

Downstream 100 bases:

>100_bases
TTTAAATTAAAAAATTTTTTTATTTTTTATTTTTTAGTTATTCTATAATTTCCAAGATCCTGTTAATTTCTTCGTTTTTA
AATATTCTTATTTTATTTCC

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKKVKVCSPGTSANLGPGYDIFGLALSNPYDIVEVEKTENGITISVEGEKAEEIPTNVDENTAGVVAKKMIEDFKIESGI
HIHIIKGIKPGSGLGSSSASCAGIAFALNELFELKLSKLELVKYSSLGEAVAAGAPHADNVAPAIFGGFTLTTSYEPLEV
LHIPVDLEVLVALPNIQVSTKTAREILPKEIPIKDMVNNVGKAAGMVYALYNNDLDLFGRYMSKDCVVEPCRANLIDGYT
EVKEKVKDMVYGITISGSGPAIITIPKKEHVIDIENIFKEVWNCPVYYTKVGPGCYVEEIE

Sequences:

>Translated_301_residues
MKKVKVCSPGTSANLGPGYDIFGLALSNPYDIVEVEKTENGITISVEGEKAEEIPTNVDENTAGVVAKKMIEDFKIESGI
HIHIIKGIKPGSGLGSSSASCAGIAFALNELFELKLSKLELVKYSSLGEAVAAGAPHADNVAPAIFGGFTLTTSYEPLEV
LHIPVDLEVLVALPNIQVSTKTAREILPKEIPIKDMVNNVGKAAGMVYALYNNDLDLFGRYMSKDCVVEPCRANLIDGYT
EVKEKVKDMVYGITISGSGPAIITIPKKEHVIDIENIFKEVWNCPVYYTKVGPGCYVEEIE
>Mature_301_residues
MKKVKVCSPGTSANLGPGYDIFGLALSNPYDIVEVEKTENGITISVEGEKAEEIPTNVDENTAGVVAKKMIEDFKIESGI
HIHIIKGIKPGSGLGSSSASCAGIAFALNELFELKLSKLELVKYSSLGEAVAAGAPHADNVAPAIFGGFTLTTSYEPLEV
LHIPVDLEVLVALPNIQVSTKTAREILPKEIPIKDMVNNVGKAAGMVYALYNNDLDLFGRYMSKDCVVEPCRANLIDGYT
EVKEKVKDMVYGITISGSGPAIITIPKKEHVIDIENIFKEVWNCPVYYTKVGPGCYVEEIE

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate [H]

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786184, Length=274, Percent_Identity=30.2919708029197, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321814, Length=359, Percent_Identity=26.4623955431755, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.39 [H]

Molecular weight: Translated: 32454; Mature: 32454

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKVCSPGTSANLGPGYDIFGLALSNPYDIVEVEKTENGITISVEGEKAEEIPTNVDE
CCCEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEEECCCCHHCCCCCCCC
NTAGVVAKKMIEDFKIESGIHIHIIKGIKPGSGLGSSSASCAGIAFALNELFELKLSKLE
CHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LVKYSSLGEAVAAGAPHADNVAPAIFGGFTLTTSYEPLEVLHIPVDLEVLVALPNIQVST
EEHHHCCCHHHHCCCCCCCCCCHHHCCCEEEECCCCCEEEEEECCCEEEEEECCCCEECH
KTAREILPKEIPIKDMVNNVGKAAGMVYALYNNDLDLFGRYMSKDCVVEPCRANLIDGYT
HHHHHHCCCCCCHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCCCEECCHHHHHHCCHH
EVKEKVKDMVYGITISGSGPAIITIPKKEHVIDIENIFKEVWNCPVYYTKVGPGCYVEEI
HHHHHHHHHEEEEEECCCCCEEEEECCCCCEECHHHHHHHHHCCCEEEEECCCCEEEEEC
E
C
>Mature Secondary Structure
MKKVKVCSPGTSANLGPGYDIFGLALSNPYDIVEVEKTENGITISVEGEKAEEIPTNVDE
CCCEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEEECCCCHHCCCCCCCC
NTAGVVAKKMIEDFKIESGIHIHIIKGIKPGSGLGSSSASCAGIAFALNELFELKLSKLE
CHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LVKYSSLGEAVAAGAPHADNVAPAIFGGFTLTTSYEPLEVLHIPVDLEVLVALPNIQVST
EEHHHCCCHHHHCCCCCCCCCCHHHCCCEEEECCCCCEEEEEECCCEEEEEECCCCEECH
KTAREILPKEIPIKDMVNNVGKAAGMVYALYNNDLDLFGRYMSKDCVVEPCRANLIDGYT
HHHHHHCCCCCCHHHHHHHHHHHHCEEEEEECCCHHHHHHHHCCCCEECCHHHHHHCCHH
EVKEKVKDMVYGITISGSGPAIITIPKKEHVIDIENIFKEVWNCPVYYTKVGPGCYVEEI
HHHHHHHHHEEEEEECCCCCEEEEECCCCCEECHHHHHHHHHCCCEEEEECCCCEEEEEC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA