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The map label for this gene is leuD [H]

Identifier: 45357699

GI number: 45357699

Start: 142844

End: 143347

Strand: Direct

Name: leuD [H]

Synonym: MMP0136

Alternate gene names: 45357699

Gene position: 142844-143347 (Clockwise)

Preceding gene: 45357698

Following gene: 45357700

Centisome position: 8.6

GC content: 37.5

Gene sequence:

>504_bases
ATGAAAAAGACTGTCGAAGGCAAAGCATGGGTTTTTGGAGATAACATCGATACTGATGCAATATTACCTGCAAGATACCT
TGTATATACTACCGAAGAACAGCTTGCAAAATATGCAATGACTGGAACCGACCCAGAATTCCCTGAAAAAGCTACAGTTG
GCGATATCATCGTTGGCGGTAAGAATTTCGGTTGCGGAAGTTCAAGAGAACACGCTCCAATTGGTTTGAAAGGTTTAGGT
ATTTCAATGGTTATTGCAGAGAGTTTTGCAAGAATTTTTTACAGAAATTCCATCAATATCGGATTCCCATTGTTAGAATG
CAAAGATATTTCAAAACATGTAAAAGAAGGCGACGTTTTGAGAGTTGACCTTGATAAAGGTACCGTTAAAAACGTTACAA
CTGGCGTTGAACTAACTGGTCAAAATCTCCCTGATTTTATGATGGAAATCTTAAACAATGGCGGATTAATGCCACACTTG
AAGAAAAAGATTTCAAAAGCTTAA

Upstream 100 bases:

>100_bases
CAGATGCTGCAATCAAAGCAAGTAAAATGCCTACAGAAATTGAATGCGACATGGAAGTACTCAGAAAAGCAATAGAAGAA
TAATTAAATCTGGTGGAATT

Downstream 100 bases:

>100_bases
ATTTTTTTATTTAGTTTTTATTTTATTATTTTTTACATTATTTTTCGGTGATTTTTATGTCAGATCCAAAAAATGTAGTT
TTTAAGGAAAGTGAGGAATT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MKKTVEGKAWVFGDNIDTDAILPARYLVYTTEEQLAKYAMTGTDPEFPEKATVGDIIVGGKNFGCGSSREHAPIGLKGLG
ISMVIAESFARIFYRNSINIGFPLLECKDISKHVKEGDVLRVDLDKGTVKNVTTGVELTGQNLPDFMMEILNNGGLMPHL
KKKISKA

Sequences:

>Translated_167_residues
MKKTVEGKAWVFGDNIDTDAILPARYLVYTTEEQLAKYAMTGTDPEFPEKATVGDIIVGGKNFGCGSSREHAPIGLKGLG
ISMVIAESFARIFYRNSINIGFPLLECKDISKHVKEGDVLRVDLDKGTVKNVTTGVELTGQNLPDFMMEILNNGGLMPHL
KKKISKA
>Mature_167_residues
MKKTVEGKAWVFGDNIDTDAILPARYLVYTTEEQLAKYAMTGTDPEFPEKATVGDIIVGGKNFGCGSSREHAPIGLKGLG
ISMVIAESFARIFYRNSINIGFPLLECKDISKHVKEGDVLRVDLDKGTVKNVTTGVELTGQNLPDFMMEILNNGGLMPHL
KKKISKA

Specific function: Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3- isopropylmalate, via the formation of 2-isopropylmaleate, a step involved

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=99, Percent_Identity=40.4040404040404, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=106, Percent_Identity=43.3962264150943, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=103, Percent_Identity=40.7766990291262, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33; =4.2.1.35; =4.2.1.31 [H]

Molecular weight: Translated: 18248; Mature: 18248

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVEGKAWVFGDNIDTDAILPARYLVYTTEEQLAKYAMTGTDPEFPEKATVGDIIVGG
CCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCEEEECC
KNFGCGSSREHAPIGLKGLGISMVIAESFARIFYRNSINIGFPLLECKDISKHVKEGDVL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEE
RVDLDKGTVKNVTTGVELTGQNLPDFMMEILNNGGLMPHLKKKISKA
EEECCCCCCCCEECCEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKKTVEGKAWVFGDNIDTDAILPARYLVYTTEEQLAKYAMTGTDPEFPEKATVGDIIVGG
CCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCEEEECC
KNFGCGSSREHAPIGLKGLGISMVIAESFARIFYRNSINIGFPLLECKDISKHVKEGDVL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEE
RVDLDKGTVKNVTTGVELTGQNLPDFMMEILNNGGLMPHLKKKISKA
EEECCCCCCCCEECCEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]