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The map label for this gene is purT [H]

Identifier: 45357686

GI number: 45357686

Start: 129070

End: 130236

Strand: Direct

Name: purT [H]

Synonym: MMP0123

Alternate gene names: 45357686

Gene position: 129070-130236 (Clockwise)

Preceding gene: 45357679

Following gene: 45357692

Centisome position: 7.77

GC content: 38.65

Gene sequence:

>1167_bases
ATGGTTGGTACTCCTCTTTTTTCAAATGCTAAGAAAATTTTACTTCTCGGAAGCGGGGAACTCGGGAAAGAAGTAATAAT
AGAAGCTCAAAGGTTTGGTGTAGAATGTATTGCAGTTGATTCTTATGAAAACGCGCCAGCAATGCAGGTAGCACATAAGT
ATCATATAATTGACATGAAGGATGCTGGAGCTTTAAGGGCGGTAATCGAAAAAGAAAAACCTGACTTAATAGTTCCTGAA
ATTGAAGCGATAAACACGGATACTCTAAAAGAAATGGAAAGCGAAGGATACCACGTGGTTCCAACTGCAAATGCGACAAA
ACTTACAATGGACAGGGAAGGAATTAGGAGACTTGCATTTGAAAAATTAGGATTAAGAACCGCAAAATACGAATTTGCAG
AAAATTTGGAAGAATTAAAAGAAGCAGTAACAAGAATTGGAATTCCATGTATCATAAAACCAATCATGTCATCATCTGGG
AAAGGGCAGAGCACGATTAAATCTGAAAGCGATATCAAAACAGCGTGGAACTATGCAAAAAGCGCTGCAAGGGGAATCGG
AACAAAGGTAATCGTTGAAGAATTTATCAAGTTTGACTATGAAATTACGCTATTAACTGCAAGAACTGCAGAAGGAACTC
GATTTTGCGAGCCAATAGGCCATATACAGGTTGACGGGGATTATCATGAAAGCTGGCAGCCTCATCCAATGTGTGCACCA
ACAAAAGCAAAAGCTCAAGAGATGGCAAAGAAGATAACTGATGAACTTGGAGGATACGGTATTTTCGGAGTAGAACTCTT
TGTTTTAGACGACGAAGTTATATTCAGTGAAGTATCCCCAAGACCTCACGATACAGGAATGGTTACAATGGTAACCCAGA
AGATGAGCGAGTTTGAAATTCACGCAAGGGCAATTTTGGGACTTCCAGTAAACGTTGATATAGTTTCTCCCGGTGCAAGC
CACGTTATAAAATCAGAAATTCTCAAATGGGCTCCAGAATATGAAATTCATGAAGCTTCGAAAGTAAAAGATACAAAAAT
AAGATTATTTGGAAAACCTATTGCAAAAGTTGGACGTAGAATGGGTGTCGCCCTTGCAGTCTCAGATGATGTAATAAAAG
CAAGAGAACATGCAGAAAAAGTAGCGCATCTTGTAAAAATTAAATAA

Upstream 100 bases:

>100_bases
AATAAGCCACGATTAAACTTATTATATATTATAACAAAATTTATTTATATTTTTTATCTATAAACTGTAAAATAACCTTA
TTAACACGCATGGTGAAACA

Downstream 100 bases:

>100_bases
TTTTTTTATTTTTTTTATTTTCAGTTGTTATGCGAGTTTTATAACGTAAAACACTAAAAGTACCCAGATTGCTATTGCAA
AATAACCTTCTTTTTTATGA

Product: phosphoribosylglycinamide formyltransferase 2

Products: tetrahydrofolate; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MVGTPLFSNAKKILLLGSGELGKEVIIEAQRFGVECIAVDSYENAPAMQVAHKYHIIDMKDAGALRAVIEKEKPDLIVPE
IEAINTDTLKEMESEGYHVVPTANATKLTMDREGIRRLAFEKLGLRTAKYEFAENLEELKEAVTRIGIPCIIKPIMSSSG
KGQSTIKSESDIKTAWNYAKSAARGIGTKVIVEEFIKFDYEITLLTARTAEGTRFCEPIGHIQVDGDYHESWQPHPMCAP
TKAKAQEMAKKITDELGGYGIFGVELFVLDDEVIFSEVSPRPHDTGMVTMVTQKMSEFEIHARAILGLPVNVDIVSPGAS
HVIKSEILKWAPEYEIHEASKVKDTKIRLFGKPIAKVGRRMGVALAVSDDVIKAREHAEKVAHLVKIK

Sequences:

>Translated_388_residues
MVGTPLFSNAKKILLLGSGELGKEVIIEAQRFGVECIAVDSYENAPAMQVAHKYHIIDMKDAGALRAVIEKEKPDLIVPE
IEAINTDTLKEMESEGYHVVPTANATKLTMDREGIRRLAFEKLGLRTAKYEFAENLEELKEAVTRIGIPCIIKPIMSSSG
KGQSTIKSESDIKTAWNYAKSAARGIGTKVIVEEFIKFDYEITLLTARTAEGTRFCEPIGHIQVDGDYHESWQPHPMCAP
TKAKAQEMAKKITDELGGYGIFGVELFVLDDEVIFSEVSPRPHDTGMVTMVTQKMSEFEIHARAILGLPVNVDIVSPGAS
HVIKSEILKWAPEYEIHEASKVKDTKIRLFGKPIAKVGRRMGVALAVSDDVIKAREHAEKVAHLVKIK
>Mature_388_residues
MVGTPLFSNAKKILLLGSGELGKEVIIEAQRFGVECIAVDSYENAPAMQVAHKYHIIDMKDAGALRAVIEKEKPDLIVPE
IEAINTDTLKEMESEGYHVVPTANATKLTMDREGIRRLAFEKLGLRTAKYEFAENLEELKEAVTRIGIPCIIKPIMSSSG
KGQSTIKSESDIKTAWNYAKSAARGIGTKVIVEEFIKFDYEITLLTARTAEGTRFCEPIGHIQVDGDYHESWQPHPMCAP
TKAKAQEMAKKITDELGGYGIFGVELFVLDDEVIFSEVSPRPHDTGMVTMVTQKMSEFEIHARAILGLPVNVDIVSPGAS
HVIKSEILKWAPEYEIHEASKVKDTKIRLFGKPIAKVGRRMGVALAVSDDVIKAREHAEKVAHLVKIK

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=55.2699228791774, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1786733, Length=200, Percent_Identity=30.5, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=315, Percent_Identity=26.031746031746, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.2

Molecular weight: Translated: 42908; Mature: 42908

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGTPLFSNAKKILLLGSGELGKEVIIEAQRFGVECIAVDSYENAPAMQVAHKYHIIDMK
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHEEEEEEEC
DAGALRAVIEKEKPDLIVPEIEAINTDTLKEMESEGYHVVPTANATKLTMDREGIRRLAF
CCCHHHHHHHCCCCCEECCCHHHCCHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHH
EKLGLRTAKYEFAENLEELKEAVTRIGIPCIIKPIMSSSGKGQSTIKSESDIKTAWNYAK
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SAARGIGTKVIVEEFIKFDYEITLLTARTAEGTRFCEPIGHIQVDGDYHESWQPHPMCAP
HHHHCCCHHHHHHHHHHCCEEEEEEEEECCCCCHHHCCCCCEEECCCCCCCCCCCCCCCC
TKAKAQEMAKKITDELGGYGIFGVELFVLDDEVIFSEVSPRPHDTGMVTMVTQKMSEFEI
CHHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHCCCCCCCCCCEEEHHHHHHHHHHE
HARAILGLPVNVDIVSPGASHVIKSEILKWAPEYEIHEASKVKDTKIRLFGKPIAKVGRR
EEEEEEECCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHH
MGVALAVSDDVIKAREHAEKVAHLVKIK
HCEEEEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVGTPLFSNAKKILLLGSGELGKEVIIEAQRFGVECIAVDSYENAPAMQVAHKYHIIDMK
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHEEEEEEEC
DAGALRAVIEKEKPDLIVPEIEAINTDTLKEMESEGYHVVPTANATKLTMDREGIRRLAF
CCCHHHHHHHCCCCCEECCCHHHCCHHHHHHHHCCCCEEEECCCCEEEEECHHHHHHHHH
EKLGLRTAKYEFAENLEELKEAVTRIGIPCIIKPIMSSSGKGQSTIKSESDIKTAWNYAK
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SAARGIGTKVIVEEFIKFDYEITLLTARTAEGTRFCEPIGHIQVDGDYHESWQPHPMCAP
HHHHCCCHHHHHHHHHHCCEEEEEEEEECCCCCHHHCCCCCEEECCCCCCCCCCCCCCCC
TKAKAQEMAKKITDELGGYGIFGVELFVLDDEVIFSEVSPRPHDTGMVTMVTQKMSEFEI
CHHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHCCCCCCCCCCEEEHHHHHHHHHHE
HARAILGLPVNVDIVSPGASHVIKSEILKWAPEYEIHEASKVKDTKIRLFGKPIAKVGRR
EEEEEEECCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHH
MGVALAVSDDVIKAREHAEKVAHLVKIK
HCEEEEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 10-formyltetrahydrofolate; N1-(5-phospho-D-ribosyl)glycinamide

Specific reaction: 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA