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The map label for this gene is pdxJ

Identifier: 42523410

GI number: 42523410

Start: 1844883

End: 1845608

Strand: Direct

Name: pdxJ

Synonym: Bd1932

Alternate gene names: 42523410

Gene position: 1844883-1845608 (Clockwise)

Preceding gene: 42523409

Following gene: 42523411

Centisome position: 48.77

GC content: 47.52

Gene sequence:

>726_bases
ATGAAACATAAAATTCGTTTGGGTGTAAATGTGGACCACGTGGCGACGCTTCGTCAGGTGCGTGGTGGGACAACGGCTTA
TCCTAATTTGCTGGATATGGTGAAAAAGTCCGTCAAAGGTGGCGCGGAACAAATCACGATTCATCTTCGTGAAGACCGCC
GTCATATTCAATTGGAAGATCTTAAAGTGCTTTCCAAGCATTGTTCAGTGCCGCTGAATCTGGAGATGGCAGCGACCTCT
CAGATGGTGACTTACGCCAAGAAATACCGTCCAGACTGGGTGTGCTTCGTCCCGGAAAAGCGTGCTGAGCTGACAACTGA
AGGCGGCCTGGATGTAAAGAAAGGCTTTAAGAAAATGTTCCCGATGGTTGAAAAGCTTCAGCGCATCGGTATTGAAATTT
CCATGTTTATTGAGCCTTCCCTTGAGCAGGTTGAAGCTTCTTATGAGATTGGTGCGGATGCCGTTGAATTCCACACCGGC
AAATGGGTGATGCTAAAAGGCGCTCGGAAAGCCGCTGAATGGAAGCGTCTTTGTGATGCCGCTGAATGGGCGCACTATCT
GGGTTTGAATGTGCACGCAGGCCACGGGCTTGATTATGAGCACTCCAAGCTTATCAATAAGTTGCCGCATCTTCAGGAAG
TGAATATTGGGCATTCTCTGGTGTGTTATGCACTAGAGCATGGTATGGAAGAATCTGTTCGTAAGATGAGAAAAATCCTG
AAATAA

Upstream 100 bases:

>100_bases
AAAGCTCAGATCACTCAATTCGCGGAAGAAATCGCCTCTTTCCTGGAAAAAGAACTGTCTTAATTAAAGGGGGATGGCAG
TTCTGCCATCCTAAAAATCT

Downstream 100 bases:

>100_bases
TATGACCATTCTGAATTCCCAACACATCCTAAAAAACCTCCAGGAGCTGAAAAGCTTCTGGCAGGATTTTTTGCCGCACC
TGAATGACCGCTGTATTTTG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKHKIRLGVNVDHVATLRQVRGGTTAYPNLLDMVKKSVKGGAEQITIHLREDRRHIQLEDLKVLSKHCSVPLNLEMAATS
QMVTYAKKYRPDWVCFVPEKRAELTTEGGLDVKKGFKKMFPMVEKLQRIGIEISMFIEPSLEQVEASYEIGADAVEFHTG
KWVMLKGARKAAEWKRLCDAAEWAHYLGLNVHAGHGLDYEHSKLINKLPHLQEVNIGHSLVCYALEHGMEESVRKMRKIL
K

Sequences:

>Translated_241_residues
MKHKIRLGVNVDHVATLRQVRGGTTAYPNLLDMVKKSVKGGAEQITIHLREDRRHIQLEDLKVLSKHCSVPLNLEMAATS
QMVTYAKKYRPDWVCFVPEKRAELTTEGGLDVKKGFKKMFPMVEKLQRIGIEISMFIEPSLEQVEASYEIGADAVEFHTG
KWVMLKGARKAAEWKRLCDAAEWAHYLGLNVHAGHGLDYEHSKLINKLPHLQEVNIGHSLVCYALEHGMEESVRKMRKIL
K
>Mature_241_residues
MKHKIRLGVNVDHVATLRQVRGGTTAYPNLLDMVKKSVKGGAEQITIHLREDRRHIQLEDLKVLSKHCSVPLNLEMAATS
QMVTYAKKYRPDWVCFVPEKRAELTTEGGLDVKKGFKKMFPMVEKLQRIGIEISMFIEPSLEQVEASYEIGADAVEFHTG
KWVMLKGARKAAEWKRLCDAAEWAHYLGLNVHAGHGLDYEHSKLINKLPHLQEVNIGHSLVCYALEHGMEESVRKMRKIL
K

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=235, Percent_Identity=43.8297872340426, Blast_Score=208, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_BDEBA (Q3V7R4)

Other databases:

- EMBL:   BX842651
- RefSeq:   NP_968790.1
- ProteinModelPortal:   Q3V7R4
- SMR:   Q3V7R4
- GeneID:   2734666
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1932
- NMPDR:   fig|264462.1.peg.1761
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q3V7R4
- ProtClustDB:   PRK05265
- BioCyc:   BBAC264462:BD1932-MONOMER
- BRENDA:   2.6.99.2
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27463; Mature: 27463

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 195-195 BINDING 10-10 BINDING 21-21 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKIRLGVNVDHVATLRQVRGGTTAYPNLLDMVKKSVKGGAEQITIHLREDRRHIQLED
CCCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
LKVLSKHCSVPLNLEMAATSQMVTYAKKYRPDWVCFVPEKRAELTTEGGLDVKKGFKKMF
HHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHH
PMVEKLQRIGIEISMFIEPSLEQVEASYEIGADAVEFHTGKWVMLKGARKAAEWKRLCDA
HHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHHHHHHHH
AEWAHYLGLNVHAGHGLDYEHSKLINKLPHLQEVNIGHSLVCYALEHGMEESVRKMRKIL
HHHHHHHCCEEECCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHCHHHHHHHHHHHH
K
C
>Mature Secondary Structure
MKHKIRLGVNVDHVATLRQVRGGTTAYPNLLDMVKKSVKGGAEQITIHLREDRRHIQLED
CCCEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
LKVLSKHCSVPLNLEMAATSQMVTYAKKYRPDWVCFVPEKRAELTTEGGLDVKKGFKKMF
HHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHH
PMVEKLQRIGIEISMFIEPSLEQVEASYEIGADAVEFHTGKWVMLKGARKAAEWKRLCDA
HHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHHHHHHHH
AEWAHYLGLNVHAGHGLDYEHSKLINKLPHLQEVNIGHSLVCYALEHGMEESVRKMRKIL
HHHHHHHCCEEECCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHCHHHHHHHHHHHH
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA