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The map label for this gene is spoU [C]

Identifier: 42522737

GI number: 42522737

Start: 1142753

End: 1143469

Strand: Direct

Name: spoU [C]

Synonym: Bd1203

Alternate gene names: 42522737

Gene position: 1142753-1143469 (Clockwise)

Preceding gene: 42522736

Following gene: 42522738

Centisome position: 30.21

GC content: 51.46

Gene sequence:

>717_bases
ATGTTCCCATATGGGCCCGAACTTGAAATCAATGCCAATCTTCGCGTGAACTACCAGCTGGTGCTGGAGAAGATCGGGCC
CCTGCTGACTGATGAACGCCGCCAGAAGATCGAAAAAGTGGTGGCGCTTCGCAATTTTGACACGGCCGTGGTGCTTGAGG
GCATCTATGACCGTGGCAATATCTCCGCCGTGATGAGATCTGCCGAGGGTCTTGGCTTTGGCAATTTCCACGTCATTGAA
ACCCAGGAAAAATTCAAAGAAGCCAACCGGGTCACCCAAGGGGCTGACAAATGGGTCGAAGTTAAAAAATGGAAAAAGAC
TGCGGACTGTGTGAAGGCTTTGAAAAATCAGGGTTACAAAATCTATGTGACTCATTTGGATGCGAACGCCAAACCTCTGC
ATGAGATTGATTTCAGCGGTAAGACAGCCCTGGTGCTTGGTAACGAGCGTGACGGTGTGACCCCTGAAATGATTGCGGCC
GCAGATCAAACGATCATCATTCCCATGACGGGCTTTGTGCAAAGCTTCAATATTTCCGTGGCCGGGGCTCTGGGGCTTTA
TCATATTTCCCAGGACCGCTTGAAACGCCGTGGAACCAACGCGTCCCTGACGGAAGAAGAGCAGGGGATCTTGCGGGCTC
ACTATTACATGAGAACCCAGGACAGCGCAGCCCAGTATCTGGAAGAGATGTTTTCCCGGGGCACTCTTAAATCCTGA

Upstream 100 bases:

>100_bases
TCGGTGGCGGTACTTTGGAGGCCCACCAGAAGAACATCACTCGCGACCTGGCAAAAAATCCGGAAGCTCTTTACAAATAG
TCCAGTCTACAGAGGTCAGA

Downstream 100 bases:

>100_bases
CTTCAAACTTGTGTCGCCCTTTAATTTCCAATAACCTTATTGGAAAGAAAGGGGACCCCGATGAAGAAACTGCTTTTGCT
TCCCGTTCTGTTTGCGGTCG

Product: putative RNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MFPYGPELEINANLRVNYQLVLEKIGPLLTDERRQKIEKVVALRNFDTAVVLEGIYDRGNISAVMRSAEGLGFGNFHVIE
TQEKFKEANRVTQGADKWVEVKKWKKTADCVKALKNQGYKIYVTHLDANAKPLHEIDFSGKTALVLGNERDGVTPEMIAA
ADQTIIIPMTGFVQSFNISVAGALGLYHISQDRLKRRGTNASLTEEEQGILRAHYYMRTQDSAAQYLEEMFSRGTLKS

Sequences:

>Translated_238_residues
MFPYGPELEINANLRVNYQLVLEKIGPLLTDERRQKIEKVVALRNFDTAVVLEGIYDRGNISAVMRSAEGLGFGNFHVIE
TQEKFKEANRVTQGADKWVEVKKWKKTADCVKALKNQGYKIYVTHLDANAKPLHEIDFSGKTALVLGNERDGVTPEMIAA
ADQTIIIPMTGFVQSFNISVAGALGLYHISQDRLKRRGTNASLTEEEQGILRAHYYMRTQDSAAQYLEEMFSRGTLKS
>Mature_238_residues
MFPYGPELEINANLRVNYQLVLEKIGPLLTDERRQKIEKVVALRNFDTAVVLEGIYDRGNISAVMRSAEGLGFGNFHVIE
TQEKFKEANRVTQGADKWVEVKKWKKTADCVKALKNQGYKIYVTHLDANAKPLHEIDFSGKTALVLGNERDGVTPEMIAA
ADQTIIIPMTGFVQSFNISVAGALGLYHISQDRLKRRGTNASLTEEEQGILRAHYYMRTQDSAAQYLEEMFSRGTLKS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=184, Percent_Identity=36.9565217391304, Blast_Score=127, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 26771; Mature: 26771

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPYGPELEINANLRVNYQLVLEKIGPLLTDERRQKIEKVVALRNFDTAVVLEGIYDRGN
CCCCCCCEEECCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
ISAVMRSAEGLGFGNFHVIETQEKFKEANRVTQGADKWVEVKKWKKTADCVKALKNQGYK
HHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCE
IYVTHLDANAKPLHEIDFSGKTALVLGNERDGVTPEMIAAADQTIIIPMTGFVQSFNISV
EEEEEECCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHCCCEEEEEEHHHHHHCCEEE
AGALGLYHISQDRLKRRGTNASLTEEEQGILRAHYYMRTQDSAAQYLEEMFSRGTLKS
EEHHHEEECCHHHHHHCCCCCCCCHHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFPYGPELEINANLRVNYQLVLEKIGPLLTDERRQKIEKVVALRNFDTAVVLEGIYDRGN
CCCCCCCEEECCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
ISAVMRSAEGLGFGNFHVIETQEKFKEANRVTQGADKWVEVKKWKKTADCVKALKNQGYK
HHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCE
IYVTHLDANAKPLHEIDFSGKTALVLGNERDGVTPEMIAAADQTIIIPMTGFVQSFNISV
EEEEEECCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHCCCEEEEEEHHHHHHCCEEE
AGALGLYHISQDRLKRRGTNASLTEEEQGILRAHYYMRTQDSAAQYLEEMFSRGTLKS
EEHHHEEECCHHHHHHCCCCCCCCHHHHHHHHHHHEEECCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]