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The map label for this gene is tyrS

Identifier: 42522726

GI number: 42522726

Start: 1129648

End: 1130865

Strand: Direct

Name: tyrS

Synonym: Bd1189

Alternate gene names: 42522726

Gene position: 1129648-1130865 (Clockwise)

Preceding gene: 42522725

Following gene: 42522727

Centisome position: 29.86

GC content: 50.9

Gene sequence:

>1218_bases
ATGATGAGTCCTCAGGAGCAGCTGGAAAGAATCAAATTCGGCACGGCCGACTTCATCAATGACGAGGACATGCTTAAAAA
ACTTAAGCGCTCGATCGAGACGAAGAAACCTTTGAATATCAAACTGGGTGCAGATCCAACACGTCCGGACATCCATCTGG
GCCACACCGTTGTGATCAACAAACTGAAAACCTTCCAGGATCTGGGGCACAAGGTGTCCTTCCTGATCGGCGATTTCACA
GCGATGATCGGGGACCCCTCCGGTAAAAACAGCACGCGTCCGATGCTGACTCGCGAAGAGATCGAGGAAAACGGCCGCTC
TTACGCAAAACAAATCTTCAAGATTCTGGATCCGGAAAAAACCGAGATCGTGTATAACTCCAGCTGGATCATGAAAATGA
CACCGGCGGAATTCATCACCATGACTTCCAAGTACACCGTGGCTCAGTTGCTAGAGCGCGAGGACTTCACCAAACGTTAC
AGATCCGGCACTCCAATCGGTATTCACGAATTCATCTATCCTTTGACTCAAGGTTACGATTCCGTGGCTTTGAAAACGGA
TGTCGAGCTGGGTGGGACGGATCAGAAGTTCAATCTTCTGGTCGGTCGTGCGATGCAGGCCGCTTACGGAATGGAAGCAC
AGTGTGTGCTGACCATGCCGATTCTGGAAGGTATCGACGGCGTGAACAAGATGTCCAAGTCTTTGGACAACTATATTTCT
GTAGTGGATACTCCAAAAGACATGTTCGGAAAAACTATGAGAATTTCCGACGAGCTGATGTACCGCTGGTACGAGCTTTT
GACTGACGTCGGTGCTGCTGGTTTGAATCAGCTGCGTGCGGATGTGGCCGAAGGCCGCAAGCACCCGCGCACAGTGAAAG
TGGAGCTGGCAAAATTCCTTATTAAACGTTTCCATTCCCAGGCTGAAGCGCAAGCGGCGGAAGACGAGTTCAACCGCATC
TTCGTGGAAAAAGGTCTGCCGGATGAAGTGCCTGACTTTGAAGTCGAAGCCGAGACCCAAATGGGGCTGGCGGCATTGAT
GGTGAAAGCCCAGCTGGCGGCTTCCAACAGTGAAGCCGGTCGCCTGATCCAGGGTGGAGGTGTTCAGATTGACGGCGAGA
AGGTTTCCGACCCTCGTTTGAAAATCGACCTGAAGTCCGGAGCAAGCTTCGTGCTGAAAGCCGGTAAAAAGAAATTCGTT
AAGATTGTTGTTAAGTAG

Upstream 100 bases:

>100_bases
TGTCCCGTGATATCGCTCGTAAGATCGAACGCGAGATGCCTCAGGCCGGTCAGGTGAAAGTCACTGTGGTTCGTGAAACC
CGTTCTGTGGAGCATGCAAG

Downstream 100 bases:

>100_bases
TCAGAACTGATCGGGGATCCGAGTTATGAAAATCAAATTTCTTCCGCAGAATATCGAAGTCGAAGGAACTCCTGACAAGA
GCCTTTTGCAGATTGCAACG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MMSPQEQLERIKFGTADFINDEDMLKKLKRSIETKKPLNIKLGADPTRPDIHLGHTVVINKLKTFQDLGHKVSFLIGDFT
AMIGDPSGKNSTRPMLTREEIEENGRSYAKQIFKILDPEKTEIVYNSSWIMKMTPAEFITMTSKYTVAQLLEREDFTKRY
RSGTPIGIHEFIYPLTQGYDSVALKTDVELGGTDQKFNLLVGRAMQAAYGMEAQCVLTMPILEGIDGVNKMSKSLDNYIS
VVDTPKDMFGKTMRISDELMYRWYELLTDVGAAGLNQLRADVAEGRKHPRTVKVELAKFLIKRFHSQAEAQAAEDEFNRI
FVEKGLPDEVPDFEVEAETQMGLAALMVKAQLAASNSEAGRLIQGGGVQIDGEKVSDPRLKIDLKSGASFVLKAGKKKFV
KIVVK

Sequences:

>Translated_405_residues
MMSPQEQLERIKFGTADFINDEDMLKKLKRSIETKKPLNIKLGADPTRPDIHLGHTVVINKLKTFQDLGHKVSFLIGDFT
AMIGDPSGKNSTRPMLTREEIEENGRSYAKQIFKILDPEKTEIVYNSSWIMKMTPAEFITMTSKYTVAQLLEREDFTKRY
RSGTPIGIHEFIYPLTQGYDSVALKTDVELGGTDQKFNLLVGRAMQAAYGMEAQCVLTMPILEGIDGVNKMSKSLDNYIS
VVDTPKDMFGKTMRISDELMYRWYELLTDVGAAGLNQLRADVAEGRKHPRTVKVELAKFLIKRFHSQAEAQAAEDEFNRI
FVEKGLPDEVPDFEVEAETQMGLAALMVKAQLAASNSEAGRLIQGGGVQIDGEKVSDPRLKIDLKSGASFVLKAGKKKFV
KIVVK
>Mature_405_residues
MMSPQEQLERIKFGTADFINDEDMLKKLKRSIETKKPLNIKLGADPTRPDIHLGHTVVINKLKTFQDLGHKVSFLIGDFT
AMIGDPSGKNSTRPMLTREEIEENGRSYAKQIFKILDPEKTEIVYNSSWIMKMTPAEFITMTSKYTVAQLLEREDFTKRY
RSGTPIGIHEFIYPLTQGYDSVALKTDVELGGTDQKFNLLVGRAMQAAYGMEAQCVLTMPILEGIDGVNKMSKSLDNYIS
VVDTPKDMFGKTMRISDELMYRWYELLTDVGAAGLNQLRADVAEGRKHPRTVKVELAKFLIKRFHSQAEAQAAEDEFNRI
FVEKGLPDEVPDFEVEAETQMGLAALMVKAQLAASNSEAGRLIQGGGVQIDGEKVSDPRLKIDLKSGASFVLKAGKKKFV
KIVVK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=418, Percent_Identity=25.5980861244019, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1787925, Length=394, Percent_Identity=28.9340101522843, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=206, Percent_Identity=31.5533980582524, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=251, Percent_Identity=23.9043824701195, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130383, Length=410, Percent_Identity=24.6341463414634, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_BDEBA (Q6MNQ2)

Other databases:

- EMBL:   BX842649
- RefSeq:   NP_968106.1
- ProteinModelPortal:   Q6MNQ2
- SMR:   Q6MNQ2
- GeneID:   2733227
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1189
- NMPDR:   fig|264462.1.peg.1076
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- PhylomeDB:   Q6MNQ2
- ProtClustDB:   PRK05912
- BioCyc:   BBAC264462:BD1189-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45369; Mature: 45369

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSPQEQLERIKFGTADFINDEDMLKKLKRSIETKKPLNIKLGADPTRPDIHLGHTVVIN
CCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCHHHHHH
KLKTFQDLGHKVSFLIGDFTAMIGDPSGKNSTRPMLTREEIEENGRSYAKQIFKILDPEK
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TEIVYNSSWIMKMTPAEFITMTSKYTVAQLLEREDFTKRYRSGTPIGIHEFIYPLTQGYD
CEEEECCCEEEEECCHHHHEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
SVALKTDVELGGTDQKFNLLVGRAMQAAYGMEAQCVLTMPILEGIDGVNKMSKSLDNYIS
CEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHH
VVDTPKDMFGKTMRISDELMYRWYELLTDVGAAGLNQLRADVAEGRKHPRTVKVELAKFL
HCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
IKRFHSQAEAQAAEDEFNRIFVEKGLPDEVPDFEVEAETQMGLAALMVKAQLAASNSEAG
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCC
RLIQGGGVQIDGEKVSDPRLKIDLKSGASFVLKAGKKKFVKIVVK
CEEECCCEEECCCCCCCCEEEEEECCCCHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MMSPQEQLERIKFGTADFINDEDMLKKLKRSIETKKPLNIKLGADPTRPDIHLGHTVVIN
CCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCEECCHHHHHH
KLKTFQDLGHKVSFLIGDFTAMIGDPSGKNSTRPMLTREEIEENGRSYAKQIFKILDPEK
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TEIVYNSSWIMKMTPAEFITMTSKYTVAQLLEREDFTKRYRSGTPIGIHEFIYPLTQGYD
CEEEECCCEEEEECCHHHHEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
SVALKTDVELGGTDQKFNLLVGRAMQAAYGMEAQCVLTMPILEGIDGVNKMSKSLDNYIS
CEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHH
VVDTPKDMFGKTMRISDELMYRWYELLTDVGAAGLNQLRADVAEGRKHPRTVKVELAKFL
HCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
IKRFHSQAEAQAAEDEFNRIFVEKGLPDEVPDFEVEAETQMGLAALMVKAQLAASNSEAG
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCC
RLIQGGGVQIDGEKVSDPRLKIDLKSGASFVLKAGKKKFVKIVVK
CEEECCCEEECCCCCCCCEEEEEECCCCHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA