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The map label for this gene is ilvE [H]

Identifier: 42522679

GI number: 42522679

Start: 1072642

End: 1073709

Strand: Direct

Name: ilvE [H]

Synonym: Bd1131

Alternate gene names: 42522679

Gene position: 1072642-1073709 (Clockwise)

Preceding gene: 42522676

Following gene: 42522681

Centisome position: 28.35

GC content: 52.15

Gene sequence:

>1068_bases
ATGACCACAATTACGAAGAATCTAACCAACACTCCGAAAGCCCTTCCACCCTCTGATCAGCTTGGATTCGGGCAGCATTT
TTCTGACCACATGTTTGTGGCTAAATACACTGAAGGCAAGGGCTGGTCTGAAGCCAGCGTCGTCCCTTATGGGCCGCTTT
CTTTGGATCCCGGGGCTTCGGTGTTTCATTATGGTCAGGCTTTGTTTGAAGGCATGAAGGCCTTCCGCAAAGAAAGCGGC
GAAGTGGTCTTCTTCCGTCCTGAGTTCAATTATCACCGTTTGGCTGAAGGCGCTGCTCGTTTGTGCCTGGAAGCTCCGCC
AATGGACCTTTTCATGGAAGGTCTGCACCAGGTGGTGGCGGCTGATGAGCGCTGGATTCCAAAAGGGCCGAACACGTCTT
TGTATATCCGTCCGACCTTGATTGGGTCTGAGCCGTTCCTGGGGGTTCGTCCTTCCCGTGAAACGTTGTTCTTTATTCTG
TTGTCTCCGGTGGGTTCTTATTATTCTGAAGGCACAAAACCAATCAAGATTTGGACCGAGGAAAAGTACCTCCGTGCAGC
TCCAGGGGGCCTGGGTGCGGTGAAGGCCGGGGCGAACTATGCCTCGAGTTTAAAGGCGGCCTTGGAAGCCAAGAAGAAGG
GCTATGCCCAAGTTCTGTGGCTGGATGTCGAGCACCAGGGTATTGAGGAGGTCGGCACAATGAACGTGTTCTTCGTCTTC
GATAATGAAATCGTCACGCCGGCATTGAATGGCAGCATTCTTTCTGGTGGCACCCGCGATGCGATTCTGCAGTTGTTGCG
CTCTAAAAATCTGCCAGTGGTGGAAAGACGTATCACGATCACGGAAGTGGTGGATCGTCTGGCAAAAGGTGAATTGAAAG
AAGCTTTCGGCACGGGTACGGCGGCGGTGATTTCCCCAGTGGGTGTTTTGCACTATCAGGGGAAAGACTGGACAATCAAC
AACAACGAAAACGGACCTCTGAGCACACAGTTGTACAACGAGATAACGGCGATCCAGCGTGGCGCTCAAGCCGATACCTT
GAACTGGCTTGTTCCTTTGAAGAAGTAA

Upstream 100 bases:

>100_bases
AGATTGCAGGGCGTTAGCCGAGGCCGGGGACTGCCTGTCAAAATGTAAAAAAGGCCAATGGATGTTTGACCATTCCGACC
ATGTTCGATAGGTCCTTTCT

Downstream 100 bases:

>100_bases
TTTTTCAGGGATTTTGTCTTAAGAAATTCGTTCGCCAAAAAAAGTGCTAGCGAGGAATCGTCAGTGATAACTCGCTAGCT
AAGGGGAATGAAACCAATCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTTITKNLTNTPKALPPSDQLGFGQHFSDHMFVAKYTEGKGWSEASVVPYGPLSLDPGASVFHYGQALFEGMKAFRKESG
EVVFFRPEFNYHRLAEGAARLCLEAPPMDLFMEGLHQVVAADERWIPKGPNTSLYIRPTLIGSEPFLGVRPSRETLFFIL
LSPVGSYYSEGTKPIKIWTEEKYLRAAPGGLGAVKAGANYASSLKAALEAKKKGYAQVLWLDVEHQGIEEVGTMNVFFVF
DNEIVTPALNGSILSGGTRDAILQLLRSKNLPVVERRITITEVVDRLAKGELKEAFGTGTAAVISPVGVLHYQGKDWTIN
NNENGPLSTQLYNEITAIQRGAQADTLNWLVPLKK

Sequences:

>Translated_355_residues
MTTITKNLTNTPKALPPSDQLGFGQHFSDHMFVAKYTEGKGWSEASVVPYGPLSLDPGASVFHYGQALFEGMKAFRKESG
EVVFFRPEFNYHRLAEGAARLCLEAPPMDLFMEGLHQVVAADERWIPKGPNTSLYIRPTLIGSEPFLGVRPSRETLFFIL
LSPVGSYYSEGTKPIKIWTEEKYLRAAPGGLGAVKAGANYASSLKAALEAKKKGYAQVLWLDVEHQGIEEVGTMNVFFVF
DNEIVTPALNGSILSGGTRDAILQLLRSKNLPVVERRITITEVVDRLAKGELKEAFGTGTAAVISPVGVLHYQGKDWTIN
NNENGPLSTQLYNEITAIQRGAQADTLNWLVPLKK
>Mature_354_residues
TTITKNLTNTPKALPPSDQLGFGQHFSDHMFVAKYTEGKGWSEASVVPYGPLSLDPGASVFHYGQALFEGMKAFRKESGE
VVFFRPEFNYHRLAEGAARLCLEAPPMDLFMEGLHQVVAADERWIPKGPNTSLYIRPTLIGSEPFLGVRPSRETLFFILL
SPVGSYYSEGTKPIKIWTEEKYLRAAPGGLGAVKAGANYASSLKAALEAKKKGYAQVLWLDVEHQGIEEVGTMNVFFVFD
NEIVTPALNGSILSGGTRDAILQLLRSKNLPVVERRITITEVVDRLAKGELKEAFGTGTAAVISPVGVLHYQGKDWTINN
NENGPLSTQLYNEITAIQRGAQADTLNWLVPLKK

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=360, Percent_Identity=40.8333333333333, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI296010906, Length=360, Percent_Identity=40.8333333333333, Blast_Score=261, Evalue=9e-70,
Organism=Homo sapiens, GI296010904, Length=360, Percent_Identity=40.8333333333333, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI50658084, Length=356, Percent_Identity=37.6404494382022, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI296010900, Length=360, Percent_Identity=37.5, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI296010902, Length=360, Percent_Identity=37.5, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=38.4353741496599, Blast_Score=205, Evalue=5e-53,
Organism=Escherichia coli, GI48994963, Length=314, Percent_Identity=29.6178343949045, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17568601, Length=345, Percent_Identity=38.8405797101449, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17565728, Length=350, Percent_Identity=32.8571428571429, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322002, Length=359, Percent_Identity=42.3398328690808, Blast_Score=269, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6322608, Length=346, Percent_Identity=41.3294797687861, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24641779, Length=343, Percent_Identity=41.1078717201166, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39002; Mature: 38871

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTITKNLTNTPKALPPSDQLGFGQHFSDHMFVAKYTEGKGWSEASVVPYGPLSLDPGAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
VFHYGQALFEGMKAFRKESGEVVFFRPEFNYHRLAEGAARLCLEAPPMDLFMEGLHQVVA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ADERWIPKGPNTSLYIRPTLIGSEPFLGVRPSRETLFFILLSPVGSYYSEGTKPIKIWTE
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECHHHHHHHCCCCEEEEEEC
EKYLRAAPGGLGAVKAGANYASSLKAALEAKKKGYAQVLWLDVEHQGIEEVGTMNVFFVF
CCEEECCCCCCCCHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCEEEEEEE
DNEIVTPALNGSILSGGTRDAILQLLRSKNLPVVERRITITEVVDRLAKGELKEAFGTGT
CCCEECCCCCCCEECCCCHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHHHHHHHHHCCCC
AAVISPVGVLHYQGKDWTINNNENGPLSTQLYNEITAIQRGAQADTLNWLVPLKK
HHHHCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCC
>Mature Secondary Structure 
TTITKNLTNTPKALPPSDQLGFGQHFSDHMFVAKYTEGKGWSEASVVPYGPLSLDPGAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCH
VFHYGQALFEGMKAFRKESGEVVFFRPEFNYHRLAEGAARLCLEAPPMDLFMEGLHQVVA
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ADERWIPKGPNTSLYIRPTLIGSEPFLGVRPSRETLFFILLSPVGSYYSEGTKPIKIWTE
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECHHHHHHHCCCCEEEEEEC
EKYLRAAPGGLGAVKAGANYASSLKAALEAKKKGYAQVLWLDVEHQGIEEVGTMNVFFVF
CCEEECCCCCCCCHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCEEEEEEE
DNEIVTPALNGSILSGGTRDAILQLLRSKNLPVVERRITITEVVDRLAKGELKEAFGTGT
CCCEECCCCCCCEECCCCHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHHHHHHHHHCCCC
AAVISPVGVLHYQGKDWTINNNENGPLSTQLYNEITAIQRGAQADTLNWLVPLKK
HHHHCCCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]