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The map label for this gene is bfmBAA [H]

Identifier: 42522535

GI number: 42522535

Start: 920241

End: 921371

Strand: Direct

Name: bfmBAA [H]

Synonym: Bd0972

Alternate gene names: 42522535

Gene position: 920241-921371 (Clockwise)

Preceding gene: 42522534

Following gene: 42522536

Centisome position: 24.33

GC content: 51.37

Gene sequence:

>1131_bases
ATGGCTAAAAAGACCACTGTGAAAGCAGGGGGTAAGGCGGCTGCTGGTAAGACTACCAAGAAGGCTGCAAAACCTGCTGC
AAAGTCCACTGTGAAAGCTTCTAAAAAAGCGACTGCTCCTGCAAAAAAGAAGCAGAGCTTTGGTGGATTGTCTGAAGAAA
TTTTGCTTCGCATGCATAACCTGATGGTTAAATCCCGCGTGCTTGAAGAACGTCTGATCAAAATTTATAAAGCCGGCGAG
GCTTACTTCTGGATCGGTGGACCTGGTGAGGAAGCCTGGGGTGTTGCCTTGGGTATGCTGGCGCGCAAAGGCTCTGGCCC
AGAGAACGACTGGTTGCACCTGCACTATCGCTGTACTCCGACGATGGTGGCTTTGGGCATGGACATGATTGATTCCATCC
GCTTGATGATGAACCGTGCGACGGATCCATCCACAGGTGGTCGTAACTTCTCCAGTCACTATTGCTTCCCGCAATGGAAC
GTGGCTCCGGTGACGTCTCCTTTGGAAGTTCAATATCCGATCGCGTGCGGTACGGCTCACGTGCAAAAGCGGGCGGGTAA
AGGTGCGATCTCCATCGTGACTGGTGGTGACGCTGGGACTGCTGAAGGTGATTTTGCATCCTGCTTGATTCTTGCCTCCC
GTAAGGGCCAGGAACTTCCGATGCTGATCACTGTACAGAACAACGGCTACGGTATTTCGACTCCGTACGAAGGTCAGCAC
GGTGAAACGAACATCGCGGATCGCGCTGCTGCGTTCAACATCCGTTCCCGTGTGATCAACGGCAATGACCCGATTGAAAC
ATATCTGGCTCTGAAAGAAGAAATGGAATACATCCGTAAAACCGGCAAACCATCCTTCATCGAAGCGAAGGTGACTCGTT
TGTACGGTCACTCTTCCGCGGACGGCGCCAATAAAAAACCACACTTGTTTGACCCGGTTCTGACTTTCGAAAAAAACCTG
ATCGACGCTGGCATTCTGGATCCGAAGGTGGCGAAGAAGATCTGGGAAGATTATGAAGCCGAAGGCGTCAGCGCTCAGGA
ACAAGCCCGCCAGGAACCGGTACCGACTCCGGAATCTGTTTGGGATCATGTTTACGTTAACAGCGAAAATGCAGATTGGA
GAAAATTCTAA

Upstream 100 bases:

>100_bases
CCCTTTAAAATTGATTTGAAGAGACTTCTCACCGTGGCAAATGGGCGTGACAAAAGCCTCTCTTTTCGCTTAGATAGACG
CGAATATGGAGGCCCTGAAA

Downstream 100 bases:

>100_bases
TGGCAAGTATTGCTCAGGCCATCAGAATGGCCCTTCACTATGGTGAAGCCAACATGGGAGTTAAAGACATCTTCGGTCAG
GACGTGGGCGCACCACTGGG

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAKKTTVKAGGKAAAGKTTKKAAKPAAKSTVKASKKATAPAKKKQSFGGLSEEILLRMHNLMVKSRVLEERLIKIYKAGE
AYFWIGGPGEEAWGVALGMLARKGSGPENDWLHLHYRCTPTMVALGMDMIDSIRLMMNRATDPSTGGRNFSSHYCFPQWN
VAPVTSPLEVQYPIACGTAHVQKRAGKGAISIVTGGDAGTAEGDFASCLILASRKGQELPMLITVQNNGYGISTPYEGQH
GETNIADRAAAFNIRSRVINGNDPIETYLALKEEMEYIRKTGKPSFIEAKVTRLYGHSSADGANKKPHLFDPVLTFEKNL
IDAGILDPKVAKKIWEDYEAEGVSAQEQARQEPVPTPESVWDHVYVNSENADWRKF

Sequences:

>Translated_376_residues
MAKKTTVKAGGKAAAGKTTKKAAKPAAKSTVKASKKATAPAKKKQSFGGLSEEILLRMHNLMVKSRVLEERLIKIYKAGE
AYFWIGGPGEEAWGVALGMLARKGSGPENDWLHLHYRCTPTMVALGMDMIDSIRLMMNRATDPSTGGRNFSSHYCFPQWN
VAPVTSPLEVQYPIACGTAHVQKRAGKGAISIVTGGDAGTAEGDFASCLILASRKGQELPMLITVQNNGYGISTPYEGQH
GETNIADRAAAFNIRSRVINGNDPIETYLALKEEMEYIRKTGKPSFIEAKVTRLYGHSSADGANKKPHLFDPVLTFEKNL
IDAGILDPKVAKKIWEDYEAEGVSAQEQARQEPVPTPESVWDHVYVNSENADWRKF
>Mature_375_residues
AKKTTVKAGGKAAAGKTTKKAAKPAAKSTVKASKKATAPAKKKQSFGGLSEEILLRMHNLMVKSRVLEERLIKIYKAGEA
YFWIGGPGEEAWGVALGMLARKGSGPENDWLHLHYRCTPTMVALGMDMIDSIRLMMNRATDPSTGGRNFSSHYCFPQWNV
APVTSPLEVQYPIACGTAHVQKRAGKGAISIVTGGDAGTAEGDFASCLILASRKGQELPMLITVQNNGYGISTPYEGQHG
ETNIADRAAAFNIRSRVINGNDPIETYLALKEEMEYIRKTGKPSFIEAKVTRLYGHSSADGANKKPHLFDPVLTFEKNLI
DAGILDPKVAKKIWEDYEAEGVSAQEQARQEPVPTPESVWDHVYVNSENADWRKF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=328, Percent_Identity=25.9146341463415, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI258645172, Length=328, Percent_Identity=25.9146341463415, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI86563355, Length=255, Percent_Identity=27.843137254902, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI86563357, Length=255, Percent_Identity=27.843137254902, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355903, Length=258, Percent_Identity=28.6821705426357, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 41055; Mature: 40924

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKTTVKAGGKAAAGKTTKKAAKPAAKSTVKASKKATAPAKKKQSFGGLSEEILLRMHN
CCCCCEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHH
LMVKSRVLEERLIKIYKAGEAYFWIGGPGEEAWGVALGMLARKGSGPENDWLHLHYRCTP
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCH
TMVALGMDMIDSIRLMMNRATDPSTGGRNFSSHYCFPQWNVAPVTSPLEVQYPIACGTAH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECEECCHHH
VQKRAGKGAISIVTGGDAGTAEGDFASCLILASRKGQELPMLITVQNNGYGISTPYEGQH
HHHHCCCCEEEEEECCCCCCCCCCHHEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCC
GETNIADRAAAFNIRSRVINGNDPIETYLALKEEMEYIRKTGKPSFIEAKVTRLYGHSSA
CCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
DGANKKPHLFDPVLTFEKNLIDAGILDPKVAKKIWEDYEAEGVSAQEQARQEPVPTPESV
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
WDHVYVNSENADWRKF
HCEEEECCCCCCCCCC
>Mature Secondary Structure 
AKKTTVKAGGKAAAGKTTKKAAKPAAKSTVKASKKATAPAKKKQSFGGLSEEILLRMHN
CCCCEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHH
LMVKSRVLEERLIKIYKAGEAYFWIGGPGEEAWGVALGMLARKGSGPENDWLHLHYRCTP
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCH
TMVALGMDMIDSIRLMMNRATDPSTGGRNFSSHYCFPQWNVAPVTSPLEVQYPIACGTAH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECEECCHHH
VQKRAGKGAISIVTGGDAGTAEGDFASCLILASRKGQELPMLITVQNNGYGISTPYEGQH
HHHHCCCCEEEEEECCCCCCCCCCHHEEEEEECCCCCCCCEEEEEECCCEECCCCCCCCC
GETNIADRAAAFNIRSRVINGNDPIETYLALKEEMEYIRKTGKPSFIEAKVTRLYGHSSA
CCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
DGANKKPHLFDPVLTFEKNLIDAGILDPKVAKKIWEDYEAEGVSAQEQARQEPVPTPESV
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
WDHVYVNSENADWRKF
HCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]