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The map label for this gene is pilD [H]

Identifier: 42522434

GI number: 42522434

Start: 809503

End: 810282

Strand: Direct

Name: pilD [H]

Synonym: Bd0862

Alternate gene names: 42522434

Gene position: 809503-810282 (Clockwise)

Preceding gene: 42522433

Following gene: 42522435

Centisome position: 21.4

GC content: 51.28

Gene sequence:

>780_bases
CTGGATACGGGCTTTTACGTCCTTTTCTTTGTTTTGGGTGCGATTTTTGGAAGTTTCGGCAATGTCGTGATCTATCGCCT
GCCGCGCGAGGAAAGTGTCGTCAAGCCTCGCAGCTACTGTTACGGCTGCAAAACCCAGATCAAATGGTATGACAATATCC
CTATCCTGAGCTGGTTCATTCTTCGTGGCAAATGCCGCAAGTGTCAGGCCAAGTTCTCTTTCCGCTACCCTCTGGTGGAA
ATCATCATGGCGGTGCTGTTTGCGCTTTCTTACCACTATGCGGGTCTGACTTGGACGCTGCTTGAATACTTGATCTTTAT
TTTTGGACTTGTTGTCTGCACGTTCATCGATCTGGATCACATGATCCTGCCGGACGAATTTACCCTGTCAGGGATTGTCA
TTGGACTTGTCGGTGCAGCCTTGAATCCCCAGCGTGAGTTCCTGGATGCGCTGTTTGGCGTGTTGATGGGCGGGGGCTTC
CTTTGGGGGATGGCTTACGTCTATTATATGTTCACCAAAAATGAAGGCATGGGTGGGGGCGATATCAAGCTTCTGGCCTG
GATCGGTGCCATCGTCGGCTGGAAAGCCATTCCGTTTGTGATCATGACCTCGGCGATTGTGGGCAGTGTGATCGGTTTGA
TCGCTGCCAGAAAGCAGAAAGCTGGACTAAAGACGGTGATTCCGTTCGGTCCGTACCTGGCTTTGGGAGCTGTGGTTTAT
CTTTTTGGCGGTGAAGCCATCGCCCTTTGGTACCTCGGTTTGTTCCTTCCCGCTGTCTAA

Upstream 100 bases:

>100_bases
GCTGAAAATATTCTGTGGATGCTGGCGCACATGACGGGCTGGGCTTTGTTGGCGGTGCTGGCGACGAACTTCTTCTTCAG
GAGAAAAGACATTGTTTGAT

Downstream 100 bases:

>100_bases
TCTGAAGGCAAGACCTTCGCCCCGGGGCTGGACCTTTTGGTTTCAGCCTCGACCCTTGTTTGACATTCCCCACCCAAGCA
GAAATAATTTTAGTATATCA

Product: leader peptidase (prepilin peptidase)

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDTGFYVLFFVLGAIFGSFGNVVIYRLPREESVVKPRSYCYGCKTQIKWYDNIPILSWFILRGKCRKCQAKFSFRYPLVE
IIMAVLFALSYHYAGLTWTLLEYLIFIFGLVVCTFIDLDHMILPDEFTLSGIVIGLVGAALNPQREFLDALFGVLMGGGF
LWGMAYVYYMFTKNEGMGGGDIKLLAWIGAIVGWKAIPFVIMTSAIVGSVIGLIAARKQKAGLKTVIPFGPYLALGAVVY
LFGGEAIALWYLGLFLPAV

Sequences:

>Translated_259_residues
MDTGFYVLFFVLGAIFGSFGNVVIYRLPREESVVKPRSYCYGCKTQIKWYDNIPILSWFILRGKCRKCQAKFSFRYPLVE
IIMAVLFALSYHYAGLTWTLLEYLIFIFGLVVCTFIDLDHMILPDEFTLSGIVIGLVGAALNPQREFLDALFGVLMGGGF
LWGMAYVYYMFTKNEGMGGGDIKLLAWIGAIVGWKAIPFVIMTSAIVGSVIGLIAARKQKAGLKTVIPFGPYLALGAVVY
LFGGEAIALWYLGLFLPAV
>Mature_259_residues
MDTGFYVLFFVLGAIFGSFGNVVIYRLPREESVVKPRSYCYGCKTQIKWYDNIPILSWFILRGKCRKCQAKFSFRYPLVE
IIMAVLFALSYHYAGLTWTLLEYLIFIFGLVVCTFIDLDHMILPDEFTLSGIVIGLVGAALNPQREFLDALFGVLMGGGF
LWGMAYVYYMFTKNEGMGGGDIKLLAWIGAIVGWKAIPFVIMTSAIVGSVIGLIAARKQKAGLKTVIPFGPYLALGAVVY
LFGGEAIALWYLGLFLPAV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=259, Percent_Identity=37.8378378378378, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1789732, Length=237, Percent_Identity=27.8481012658228, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 28795; Mature: 28795

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure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HHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9826339 [H]