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The map label for this gene is kpsF [C]

Identifier: 42522385

GI number: 42522385

Start: 759304

End: 760284

Strand: Direct

Name: kpsF [C]

Synonym: Bd0809

Alternate gene names: 42522385

Gene position: 759304-760284 (Clockwise)

Preceding gene: 229221137

Following gene: 42522386

Centisome position: 20.07

GC content: 52.91

Gene sequence:

>981_bases
ATGTCCAAAGTTATTCAACAGGGTCTGAAAGTTCTAGAGGTTGAGGCCCAGGCCATTCTGGCATTGAAAGAGCGCCTTGG
GGACTCTTTTGAACAAGTGGTGAAGATGATCACAGCCTGTGACGGCAAAATCGTTCTGACGGGCATGGGTAAATCCGGCC
AGATCGCCAGAAAGCTTGCCAGCACTTTTTCTTCCACCGGAACTCCGGCGGTGTTTCTGCATCCGGCAGAAAGCTCGCAC
GGGGATTTGGGCCTTGTTGAAAACAACGATGTGGTGATTGCGCTGTCTTATGGCGGTGAGTCCCCTGAGTTCGCAGGCAT
CTTAAGATTTGTTTCCCGCAAAGGCATTCCTTTGATCGCGATCACCGGCAAACCGGAATCGTCATTGGCGAAAGCCGCTC
AAGTCACTTTGAATGTTCATGTGTCAGAAGAAGCTTGTCCATTGGGTCTTGCACCCACGGCCAGCAGTACAGCGACCCTG
GCAATGGGCGACGCTGTGGCAATGGCGGTGATGGCGGAAAAAGGCTTCAGCTCTGAAGACTTTGCTGAATTTCATCCCGG
CGGCAGTCTGGGGTATCGCCTGTTGACCCGCGTGCGCGATGTTATGCACGGTGGGGATGCCTTGCCGACAGTGACCCTGG
ATACACCGATTCGTCAGGTGTTCTCGATCATGACCCACAAGGATGTTCGCGGCGCTGCCGGTATCGTCGATGAAAAAGGC
GATTTGGTCGGGGTGATCACGGATGGTGATATCCGTCGTCGCCTGGAAAAATCCAATGACCCGCTGACCGGTCTTGCGAA
GGATCTGATGACCACCAATCCCAGAACCATTGATGCCAACGAATTGGCTGAAAAAGCCTTGTTCGTCATGGAGCAGTTCC
AAATTCAAATGGTCTTTGTGCTGGATAAAGAGTCCTCAAATCCGCGCAAACCCGTGGGAATCCTGCACATCCAGGATCTA
TTGCGCGCCAAAGTTCGTTAA

Upstream 100 bases:

>100_bases
TCCATCCGGAATTCAAATCAAAGCCCTTGGCTCCGCACCCTCTCTTCGTGCATTTTGTTAAAGCAAGCCTGAAGAAGAAG
TAAGAAAGGTTCGTTAGAAT

Downstream 100 bases:

>100_bases
TTTGTGAGGCCGCCTTTGGTTGTCACAAAAGGCGGCTTTGATTCCTAATACCTGAAAACAGGCAAAACGCAGGAGTCTGA
TATGTCCCTAAAGAAGTCAT

Product: polysialic acid capsule expression protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSKVIQQGLKVLEVEAQAILALKERLGDSFEQVVKMITACDGKIVLTGMGKSGQIARKLASTFSSTGTPAVFLHPAESSH
GDLGLVENNDVVIALSYGGESPEFAGILRFVSRKGIPLIAITGKPESSLAKAAQVTLNVHVSEEACPLGLAPTASSTATL
AMGDAVAMAVMAEKGFSSEDFAEFHPGGSLGYRLLTRVRDVMHGGDALPTVTLDTPIRQVFSIMTHKDVRGAAGIVDEKG
DLVGVITDGDIRRRLEKSNDPLTGLAKDLMTTNPRTIDANELAEKALFVMEQFQIQMVFVLDKESSNPRKPVGILHIQDL
LRAKVR

Sequences:

>Translated_326_residues
MSKVIQQGLKVLEVEAQAILALKERLGDSFEQVVKMITACDGKIVLTGMGKSGQIARKLASTFSSTGTPAVFLHPAESSH
GDLGLVENNDVVIALSYGGESPEFAGILRFVSRKGIPLIAITGKPESSLAKAAQVTLNVHVSEEACPLGLAPTASSTATL
AMGDAVAMAVMAEKGFSSEDFAEFHPGGSLGYRLLTRVRDVMHGGDALPTVTLDTPIRQVFSIMTHKDVRGAAGIVDEKG
DLVGVITDGDIRRRLEKSNDPLTGLAKDLMTTNPRTIDANELAEKALFVMEQFQIQMVFVLDKESSNPRKPVGILHIQDL
LRAKVR
>Mature_325_residues
SKVIQQGLKVLEVEAQAILALKERLGDSFEQVVKMITACDGKIVLTGMGKSGQIARKLASTFSSTGTPAVFLHPAESSHG
DLGLVENNDVVIALSYGGESPEFAGILRFVSRKGIPLIAITGKPESSLAKAAQVTLNVHVSEEACPLGLAPTASSTATLA
MGDAVAMAVMAEKGFSSEDFAEFHPGGSLGYRLLTRVRDVMHGGDALPTVTLDTPIRQVFSIMTHKDVRGAAGIVDEKGD
LVGVITDGDIRRRLEKSNDPLTGLAKDLMTTNPRTIDANELAEKALFVMEQFQIQMVFVLDKESSNPRKPVGILHIQDLL
RAKVR

Specific function: Unknown

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=320, Percent_Identity=45.3125, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI87082151, Length=322, Percent_Identity=40.3726708074534, Blast_Score=234, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 34818; Mature: 34686

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVIQQGLKVLEVEAQAILALKERLGDSFEQVVKMITACDGKIVLTGMGKSGQIARKLA
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
STFSSTGTPAVFLHPAESSHGDLGLVENNDVVIALSYGGESPEFAGILRFVSRKGIPLIA
HHHHCCCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEE
ITGKPESSLAKAAQVTLNVHVSEEACPLGLAPTASSTATLAMGDAVAMAVMAEKGFSSED
EECCCHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCC
FAEFHPGGSLGYRLLTRVRDVMHGGDALPTVTLDTPIRQVFSIMTHKDVRGAAGIVDEKG
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCEECCC
DLVGVITDGDIRRRLEKSNDPLTGLAKDLMTTNPRTIDANELAEKALFVMEQFQIQMVFV
CEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHEEEEEEE
LDKESSNPRKPVGILHIQDLLRAKVR
EECCCCCCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVIQQGLKVLEVEAQAILALKERLGDSFEQVVKMITACDGKIVLTGMGKSGQIARKLA
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
STFSSTGTPAVFLHPAESSHGDLGLVENNDVVIALSYGGESPEFAGILRFVSRKGIPLIA
HHHHCCCCCEEEEECCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEE
ITGKPESSLAKAAQVTLNVHVSEEACPLGLAPTASSTATLAMGDAVAMAVMAEKGFSSED
EECCCHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCC
FAEFHPGGSLGYRLLTRVRDVMHGGDALPTVTLDTPIRQVFSIMTHKDVRGAAGIVDEKG
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCEECCC
DLVGVITDGDIRRRLEKSNDPLTGLAKDLMTTNPRTIDANELAEKALFVMEQFQIQMVFV
CEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHEEEEEEE
LDKESSNPRKPVGILHIQDLLRAKVR
EECCCCCCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]