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The map label for this gene is thiP [H]

Identifier: 42522188

GI number: 42522188

Start: 550930

End: 552393

Strand: Direct

Name: thiP [H]

Synonym: Bd0594

Alternate gene names: 42522188

Gene position: 550930-552393 (Clockwise)

Preceding gene: 42522187

Following gene: 42522189

Centisome position: 14.56

GC content: 51.09

Gene sequence:

>1464_bases
TTGAACCTGCGCCAGTGCCTCAGAATTGGCCTGGTGCTGTTTCTGATTTTTCCATTCCTGTTTTTGCTGACCCAGTTTGG
CTTTTCCGGCTGGCCCGATATGGGCGAGCTGGTTTGGGCGTTTAAAAACAGTTTCCTGCAGGCATTCTTTTCTTCCGTCT
TTTCATTGATCATGGGTTTTTGGGTGGCGCTGGGTTTGTTGACTCTGCACTCGGCCCGCGGTCGCAAAATTCGCTGGGGG
CTTGAGATTCTGTGCCTGCTGCCGAACTTTATGCCGCCGATCTTTATTCTGCTTTCCACTTTGAATGTGATTGATCCGTT
TCCGATGGGAATCCCCGGGATTGTGATCGTGCACACATTAATGAATTTCGGTCTGGTGGCGGTTTTGTTGGCGTCGACCA
TTGAAAACAAACTGGGCGGCATGATCGAACTTGCTTATGTGGAAGGGGCGGGTCGGTGGCAGTTCCTGCTGCGCGGGCTT
TTCCCGATGTTGAAAAAAGACTTCTGGCTGCTGGGTTTATTTATTTTTGTGATCTGTTTTGGCAGCTTCGCGGTGCCGTT
GATCGTGGGCGGCGGGCGTGGCACCACCGTGGAAGTTTTGATCTATGAAAAGATCCGGCTTTCCAGCAACTGGGGGGACG
CCGTTTTACTGGCCTTCCTGCAATCGGTGTTTATCTTTGCCTTGTCCTTTGTTGCCAGCCGTGGAAAAGGAATGCACAGC
ACCCGCACGGCGAACTTGAGCCTGCTGAAAACACCGAGCGGAATTTTTGTTATTGCCGGTTTGTCTTTATTGTACCTGTT
TGGGTATGTGCAGGGCTTCTTGTCAGGACTTTCGCAGATTTCTACTTTCTATGAGTTGCAGTCCGCTTTGATCTGGAACT
TTATCGGCAGTCTGACCTTGGGAATGAGCGTGGGAATTCTGTCTTATGCCGGTTTGCTGCTGATTGCTTACTGTTGGCCA
AAGGCTTGGTTTGAAAGATTCCTGAACGGGTATGTGGCGCCGTCGACTTCATTGGCGTGTTTTTGTCTTTTGATCCTGGG
CCCCAATGAGGGTTTTTATCCGTTCATCAAAATTCCGGTGGCGTTGACCCTGCTCAGTCTGAACAGTTTGTTCCGCATGG
GCTGGGACAGTGAACTGCACGCTTTGCAGAATCAGATGACGGTCGCTTATTCCATGGGGGCTTCAAAAGCCCAGATCTTT
AAAGAGATCCTGTTCCCGCAGCTTTCAGGGCGTGCGGGTTTGCTGGCGGGGATTGCGTCGATCTGGGCTGCCGGTGACTT
TGCGGTGTCGCGTATTCTGGCGCACCGGGATCTGAGCATCGCCATGATGACGGAAACTTTGATGTCAGGTTATCGCCTGA
ATCAGGCCATTGTTCTTAGCAGTTTGATTATTGTCGCCGGTTTGATTTGTTTTGCATTGTGTGTGGGAGGCAGCCGTGTC
CTTGGTCGAAAATTTGCACCGTGA

Upstream 100 bases:

>100_bases
TTATGAATGTCAAAACCATGCAACAGGTTGAGATTCTTTCTTCCACCGAAGTGGACCGCCTGCTTCGCCGTTGGACAGAG
ATCCGCCGGGGCGATCTCAA

Downstream 100 bases:

>100_bases
TTACGGAGACTTCAAGTTAGACATCGATTCCTGGGAAATCCTGGATGAAGGTGTCACTGTGCTATGGGGGCCGTCAGGCT
CCGGGAAAACCTCGGTGTTT

Product: thiamine transport system permease protein ThiP

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MNLRQCLRIGLVLFLIFPFLFLLTQFGFSGWPDMGELVWAFKNSFLQAFFSSVFSLIMGFWVALGLLTLHSARGRKIRWG
LEILCLLPNFMPPIFILLSTLNVIDPFPMGIPGIVIVHTLMNFGLVAVLLASTIENKLGGMIELAYVEGAGRWQFLLRGL
FPMLKKDFWLLGLFIFVICFGSFAVPLIVGGGRGTTVEVLIYEKIRLSSNWGDAVLLAFLQSVFIFALSFVASRGKGMHS
TRTANLSLLKTPSGIFVIAGLSLLYLFGYVQGFLSGLSQISTFYELQSALIWNFIGSLTLGMSVGILSYAGLLLIAYCWP
KAWFERFLNGYVAPSTSLACFCLLILGPNEGFYPFIKIPVALTLLSLNSLFRMGWDSELHALQNQMTVAYSMGASKAQIF
KEILFPQLSGRAGLLAGIASIWAAGDFAVSRILAHRDLSIAMMTETLMSGYRLNQAIVLSSLIIVAGLICFALCVGGSRV
LGRKFAP

Sequences:

>Translated_487_residues
MNLRQCLRIGLVLFLIFPFLFLLTQFGFSGWPDMGELVWAFKNSFLQAFFSSVFSLIMGFWVALGLLTLHSARGRKIRWG
LEILCLLPNFMPPIFILLSTLNVIDPFPMGIPGIVIVHTLMNFGLVAVLLASTIENKLGGMIELAYVEGAGRWQFLLRGL
FPMLKKDFWLLGLFIFVICFGSFAVPLIVGGGRGTTVEVLIYEKIRLSSNWGDAVLLAFLQSVFIFALSFVASRGKGMHS
TRTANLSLLKTPSGIFVIAGLSLLYLFGYVQGFLSGLSQISTFYELQSALIWNFIGSLTLGMSVGILSYAGLLLIAYCWP
KAWFERFLNGYVAPSTSLACFCLLILGPNEGFYPFIKIPVALTLLSLNSLFRMGWDSELHALQNQMTVAYSMGASKAQIF
KEILFPQLSGRAGLLAGIASIWAAGDFAVSRILAHRDLSIAMMTETLMSGYRLNQAIVLSSLIIVAGLICFALCVGGSRV
LGRKFAP
>Mature_487_residues
MNLRQCLRIGLVLFLIFPFLFLLTQFGFSGWPDMGELVWAFKNSFLQAFFSSVFSLIMGFWVALGLLTLHSARGRKIRWG
LEILCLLPNFMPPIFILLSTLNVIDPFPMGIPGIVIVHTLMNFGLVAVLLASTIENKLGGMIELAYVEGAGRWQFLLRGL
FPMLKKDFWLLGLFIFVICFGSFAVPLIVGGGRGTTVEVLIYEKIRLSSNWGDAVLLAFLQSVFIFALSFVASRGKGMHS
TRTANLSLLKTPSGIFVIAGLSLLYLFGYVQGFLSGLSQISTFYELQSALIWNFIGSLTLGMSVGILSYAGLLLIAYCWP
KAWFERFLNGYVAPSTSLACFCLLILGPNEGFYPFIKIPVALTLLSLNSLFRMGWDSELHALQNQMTVAYSMGASKAQIF
KEILFPQLSGRAGLLAGIASIWAAGDFAVSRILAHRDLSIAMMTETLMSGYRLNQAIVLSSLIIVAGLICFALCVGGSRV
LGRKFAP

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=209, Percent_Identity=28.7081339712919, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 53551; Mature: 53551

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRQCLRIGLVLFLIFPFLFLLTQFGFSGWPDMGELVWAFKNSFLQAFFSSVFSLIMGF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVALGLLTLHSARGRKIRWGLEILCLLPNFMPPIFILLSTLNVIDPFPMGIPGIVIVHTL
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
MNFGLVAVLLASTIENKLGGMIELAYVEGAGRWQFLLRGLFPMLKKDFWLLGLFIFVICF
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSFAVPLIVGGGRGTTVEVLIYEKIRLSSNWGDAVLLAFLQSVFIFALSFVASRGKGMHS
HHHHHHEEEECCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TRTANLSLLKTPSGIFVIAGLSLLYLFGYVQGFLSGLSQISTFYELQSALIWNFIGSLTL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMSVGILSYAGLLLIAYCWPKAWFERFLNGYVAPSTSLACFCLLILGPNEGFYPFIKIPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHH
ALTLLSLNSLFRMGWDSELHALQNQMTVAYSMGASKAQIFKEILFPQLSGRAGLLAGIAS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
IWAAGDFAVSRILAHRDLSIAMMTETLMSGYRLNQAIVLSSLIIVAGLICFALCVGGSRV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LGRKFAP
HCCCCCC
>Mature Secondary Structure
MNLRQCLRIGLVLFLIFPFLFLLTQFGFSGWPDMGELVWAFKNSFLQAFFSSVFSLIMGF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVALGLLTLHSARGRKIRWGLEILCLLPNFMPPIFILLSTLNVIDPFPMGIPGIVIVHTL
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
MNFGLVAVLLASTIENKLGGMIELAYVEGAGRWQFLLRGLFPMLKKDFWLLGLFIFVICF
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSFAVPLIVGGGRGTTVEVLIYEKIRLSSNWGDAVLLAFLQSVFIFALSFVASRGKGMHS
HHHHHHEEEECCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TRTANLSLLKTPSGIFVIAGLSLLYLFGYVQGFLSGLSQISTFYELQSALIWNFIGSLTL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMSVGILSYAGLLLIAYCWPKAWFERFLNGYVAPSTSLACFCLLILGPNEGFYPFIKIPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHH
ALTLLSLNSLFRMGWDSELHALQNQMTVAYSMGASKAQIFKEILFPQLSGRAGLLAGIAS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
IWAAGDFAVSRILAHRDLSIAMMTETLMSGYRLNQAIVLSSLIIVAGLICFALCVGGSRV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LGRKFAP
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]