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The map label for this gene is thiB [H]

Identifier: 42522187

GI number: 42522187

Start: 549893

End: 550933

Strand: Direct

Name: thiB [H]

Synonym: Bd0593

Alternate gene names: 42522187

Gene position: 549893-550933 (Clockwise)

Preceding gene: 42522186

Following gene: 42522188

Centisome position: 14.54

GC content: 51.59

Gene sequence:

>1041_bases
GTGAAGCACTTTGTTCTTTTCCTGACTGTAGTATTTTTGGGTCTGTTCTTGGCGGTGCTGAACCGCACGGATACGGCCGC
GCCAAACAGCTCTCTTCCCACCGTGCGTGTTTTTGGTTATGCGTCCTTCACCGGTCGCTGGGGACCGGGGCCTGCGTTGA
AGGATCTGTTTGAAAAAAGCTGCAAGTGCAAAGTGGAGTTTATCGAAGGCAGTGATTCCGGCATTCTATTGCAACGTCTG
AAAATCGAAGGTGAAAGCCTGGGCGCGGATGTTGTGGTGGGTTTGGATCAGTTTGATCTTTCCAAAGCCGTGTCCGAACA
AAGCTGGAGAAAACTAAGTCTGGGGCAGCTGGATGTCTATGATGCTGTGAAACCGGCACTGGCCAACAGCTTCTTTGTTC
CCTATGACTGGGGGGCTTTGACTTTCGTGGCCCGCAAAAACGAGCTGATGCGCATGCCGGCCTCTTTGGATGATCTGCTG
GCGCCGGAACTTGCCAAGAAAATCGCGCTGCAGGATCCCCGCACCAGTTCCCCGGGAATGCAGTTCCTGTACTGGGTGAT
TCGCTCCAAGGGCGAGGATGAAGGCTTCCGCTATCTGCAAAAGCTGATGAGCCAGGTGCACAGTTTTTCCCCGACATGGT
CCACGTCTTATGGTCTTTTCACCAACAAGCAGACCAAGCTGGTTTACTCTTATGTGACTTCGCCGTTGTATCACGAGGTC
GAGGAAAAAAAGAAAGACTATGTGGCGCTGCCGTTCAACGAAGACCTGCCAGTGCAGTTTGAATTTGTCGGCATTCCAGA
GTTCTGCCGCCACTGTGATTTGGCCGAGCAGTTTGTGAATCTGATGCTTTCCAACGAAGGCCAGAAGATCATCATGGAAA
AGAACTATATGTTCCCGGTGATGAAGGGTGTGATGGAAAACACTTCCTTTGCTCCACTTATGAATGTCAAAACCATGCAA
CAGGTTGAGATTCTTTCTTCCACCGAAGTGGACCGCCTGCTTCGCCGTTGGACAGAGATCCGCCGGGGCGATCTCAATTG
A

Upstream 100 bases:

>100_bases
CCTGGCCATTCACCTGATCCTGGCTTGGGTCGGTGCGGGACGCTGTCTGGGCTTTGACTATTATTTCTTCAAACGCCGTC
GCGGACTTTGGTGGTAGAGC

Downstream 100 bases:

>100_bases
ACCTGCGCCAGTGCCTCAGAATTGGCCTGGTGCTGTTTCTGATTTTTCCATTCCTGTTTTTGCTGACCCAGTTTGGCTTT
TCCGGCTGGCCCGATATGGG

Product: thiamine ABC transporter

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKHFVLFLTVVFLGLFLAVLNRTDTAAPNSSLPTVRVFGYASFTGRWGPGPALKDLFEKSCKCKVEFIEGSDSGILLQRL
KIEGESLGADVVVGLDQFDLSKAVSEQSWRKLSLGQLDVYDAVKPALANSFFVPYDWGALTFVARKNELMRMPASLDDLL
APELAKKIALQDPRTSSPGMQFLYWVIRSKGEDEGFRYLQKLMSQVHSFSPTWSTSYGLFTNKQTKLVYSYVTSPLYHEV
EEKKKDYVALPFNEDLPVQFEFVGIPEFCRHCDLAEQFVNLMLSNEGQKIIMEKNYMFPVMKGVMENTSFAPLMNVKTMQ
QVEILSSTEVDRLLRRWTEIRRGDLN

Sequences:

>Translated_346_residues
MKHFVLFLTVVFLGLFLAVLNRTDTAAPNSSLPTVRVFGYASFTGRWGPGPALKDLFEKSCKCKVEFIEGSDSGILLQRL
KIEGESLGADVVVGLDQFDLSKAVSEQSWRKLSLGQLDVYDAVKPALANSFFVPYDWGALTFVARKNELMRMPASLDDLL
APELAKKIALQDPRTSSPGMQFLYWVIRSKGEDEGFRYLQKLMSQVHSFSPTWSTSYGLFTNKQTKLVYSYVTSPLYHEV
EEKKKDYVALPFNEDLPVQFEFVGIPEFCRHCDLAEQFVNLMLSNEGQKIIMEKNYMFPVMKGVMENTSFAPLMNVKTMQ
QVEILSSTEVDRLLRRWTEIRRGDLN
>Mature_346_residues
MKHFVLFLTVVFLGLFLAVLNRTDTAAPNSSLPTVRVFGYASFTGRWGPGPALKDLFEKSCKCKVEFIEGSDSGILLQRL
KIEGESLGADVVVGLDQFDLSKAVSEQSWRKLSLGQLDVYDAVKPALANSFFVPYDWGALTFVARKNELMRMPASLDDLL
APELAKKIALQDPRTSSPGMQFLYWVIRSKGEDEGFRYLQKLMSQVHSFSPTWSTSYGLFTNKQTKLVYSYVTSPLYHEV
EEKKKDYVALPFNEDLPVQFEFVGIPEFCRHCDLAEQFVNLMLSNEGQKIIMEKNYMFPVMKGVMENTSFAPLMNVKTMQ
QVEILSSTEVDRLLRRWTEIRRGDLN

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=274, Percent_Identity=37.5912408759124, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39373; Mature: 39373

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFVLFLTVVFLGLFLAVLNRTDTAAPNSSLPTVRVFGYASFTGRWGPGPALKDLFEKS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHC
CKCKVEFIEGSDSGILLQRLKIEGESLGADVVVGLDQFDLSKAVSEQSWRKLSLGQLDVY
CCEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHH
DAVKPALANSFFVPYDWGALTFVARKNELMRMPASLDDLLAPELAKKIALQDPRTSSPGM
HHHHHHHHCCCCCEECCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
QFLYWVIRSKGEDEGFRYLQKLMSQVHSFSPTWSTSYGLFTNKQTKLVYSYVTSPLYHEV
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHH
EEKKKDYVALPFNEDLPVQFEFVGIPEFCRHCDLAEQFVNLMLSNEGQKIIMEKNYMFPV
HHHCCCEEEECCCCCCCEEEEEECCHHHHHHCHHHHHHHHHHHCCCCCEEEEECCCHHHH
MKGVMENTSFAPLMNVKTMQQVEILSSTEVDRLLRRWTEIRRGDLN
HHHHHCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKHFVLFLTVVFLGLFLAVLNRTDTAAPNSSLPTVRVFGYASFTGRWGPGPALKDLFEKS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHC
CKCKVEFIEGSDSGILLQRLKIEGESLGADVVVGLDQFDLSKAVSEQSWRKLSLGQLDVY
CCEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHH
DAVKPALANSFFVPYDWGALTFVARKNELMRMPASLDDLLAPELAKKIALQDPRTSSPGM
HHHHHHHHCCCCCEECCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
QFLYWVIRSKGEDEGFRYLQKLMSQVHSFSPTWSTSYGLFTNKQTKLVYSYVTSPLYHEV
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHCCHHHHHH
EEKKKDYVALPFNEDLPVQFEFVGIPEFCRHCDLAEQFVNLMLSNEGQKIIMEKNYMFPV
HHHCCCEEEECCCCCCCEEEEEECCHHHHHHCHHHHHHHHHHHCCCCCEEEEECCCHHHH
MKGVMENTSFAPLMNVKTMQQVEILSSTEVDRLLRRWTEIRRGDLN
HHHHHCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA