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The map label for this gene is nth [H]

Identifier: 42522185

GI number: 42522185

Start: 548727

End: 549392

Strand: Direct

Name: nth [H]

Synonym: Bd0591

Alternate gene names: 42522185

Gene position: 548727-549392 (Clockwise)

Preceding gene: 42522184

Following gene: 42522186

Centisome position: 14.51

GC content: 51.8

Gene sequence:

>666_bases
ATGGCGAGTAAGCAGTCTCCTGCCAAAAAGCCCTCTGCCAAGCAGGCGGGCATTCTTGCAACTATTGAACTTCTCAAGAG
ATATTACCCTGACGCTTATTGCGCCCTGAATTACACCAATCCTTTTGAGCTTCTGGTGGCCACCATTCTTTCAGCCCAGT
GCACGGATGAAAGAGTCAACATGGTGACCCCGGCGCTGTTCAAAAAATACCCAACCCCGAAGGCCATGGCAAAAGCTCCG
GTAGAGTCCCTTGAGGAACTCATCCGTTCTACGGGGTTCTATAAAAACAAAGCAAAAAATCTGAAAGCCTGTGCAACCAC
TCTGGTGGAAAAGCACCATGGCGAAGTCCCGCAAAGTCTCGAGGCTTTGGTCGAGCTGGGAGGCGTGGGTCGCAAGACCG
CCAATGTGGTGCTGGGAAATGCCTTCAATATTCCAAGCGGGATCGTTGTGGACACCCATGTGACCCGATTGGCCAACCGC
TTAGGGTGGGTGAAAACCGACAATGCGGTGATGATTGAGCGTCAATTGTCAAAGCTCGTCCCAGTTGAAGATTGGATCAT
GCTGCCGCACTGGCTGATTTCTCATGGTCGAGCTGTCTGTAAGGCGAGAAAACCTGCGTGCAGTCATTGCTTTCTCGAGG
AGACTTGCCCTAAGAAGGGTGTCTAG

Upstream 100 bases:

>100_bases
GACGGCCACGCGTTCGTATCCCGCCAGCCGGAAACTCCGGAAGAAGAAGCTCTGTGCAAAGAGGCCATGGAAGGTTGCCC
GGTTGAGGCAATCGGAAACG

Downstream 100 bases:

>100_bases
TTCACAATGGCTTCATGTTAGTTTCATTCTTTGAGAGCGTTAAATACGTAGGTCATCTTCTTCCGATCTCTTTTTTAAGA
ATCTTCCTGGGTTATTATTA

Product: endo III-related endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MASKQSPAKKPSAKQAGILATIELLKRYYPDAYCALNYTNPFELLVATILSAQCTDERVNMVTPALFKKYPTPKAMAKAP
VESLEELIRSTGFYKNKAKNLKACATTLVEKHHGEVPQSLEALVELGGVGRKTANVVLGNAFNIPSGIVVDTHVTRLANR
LGWVKTDNAVMIERQLSKLVPVEDWIMLPHWLISHGRAVCKARKPACSHCFLEETCPKKGV

Sequences:

>Translated_221_residues
MASKQSPAKKPSAKQAGILATIELLKRYYPDAYCALNYTNPFELLVATILSAQCTDERVNMVTPALFKKYPTPKAMAKAP
VESLEELIRSTGFYKNKAKNLKACATTLVEKHHGEVPQSLEALVELGGVGRKTANVVLGNAFNIPSGIVVDTHVTRLANR
LGWVKTDNAVMIERQLSKLVPVEDWIMLPHWLISHGRAVCKARKPACSHCFLEETCPKKGV
>Mature_220_residues
ASKQSPAKKPSAKQAGILATIELLKRYYPDAYCALNYTNPFELLVATILSAQCTDERVNMVTPALFKKYPTPKAMAKAPV
ESLEELIRSTGFYKNKAKNLKACATTLVEKHHGEVPQSLEALVELGGVGRKTANVVLGNAFNIPSGIVVDTHVTRLANRL
GWVKTDNAVMIERQLSKLVPVEDWIMLPHWLISHGRAVCKARKPACSHCFLEETCPKKGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=31.4606741573034, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=43.9024390243902, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1789331, Length=189, Percent_Identity=26.4550264550265, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=37.8531073446328, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=29.9465240641711, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550361, Length=185, Percent_Identity=36.7567567567568, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24326; Mature: 24195

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKQSPAKKPSAKQAGILATIELLKRYYPDAYCALNYTNPFELLVATILSAQCTDERVN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHH
MVTPALFKKYPTPKAMAKAPVESLEELIRSTGFYKNKAKNLKACATTLVEKHHGEVPQSL
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
EALVELGGVGRKTANVVLGNAFNIPSGIVVDTHVTRLANRLGWVKTDNAVMIERQLSKLV
HHHHHHCCCCCHHHHEEECCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
PVEDWIMLPHWLISHGRAVCKARKPACSHCFLEETCPKKGV
CHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASKQSPAKKPSAKQAGILATIELLKRYYPDAYCALNYTNPFELLVATILSAQCTDERVN
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHH
MVTPALFKKYPTPKAMAKAPVESLEELIRSTGFYKNKAKNLKACATTLVEKHHGEVPQSL
HHHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
EALVELGGVGRKTANVVLGNAFNIPSGIVVDTHVTRLANRLGWVKTDNAVMIERQLSKLV
HHHHHHCCCCCHHHHEEECCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
PVEDWIMLPHWLISHGRAVCKARKPACSHCFLEETCPKKGV
CHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]