Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 42522155

GI number: 42522155

Start: 515494

End: 516450

Strand: Direct

Name: ispB [H]

Synonym: Bd0557

Alternate gene names: 42522155

Gene position: 515494-516450 (Clockwise)

Preceding gene: 42522151

Following gene: 42522156

Centisome position: 13.63

GC content: 51.72

Gene sequence:

>957_bases
TTGCAACGCAGCGTCATTGATCTTAAAGTCTATGATCCAAAGGATTTTCCGGCGTACCTGCCAAAGCTGAACAAGCTTTA
CGAGGACCTGTTTTCCGGCGGCAAAGGCTTCCGCGCAAAATTGATCCGCATGATGTCGGCTCACCTGGGCATTGAACCTA
AAACTGAGCTGCTCTTGTCCCAGACAATCGAATTCATTCATAACGCCTCTTTGCTTCATGATGATCTGATCGACCGCTCT
CATCTTCGCCGTGGCAAGACAGCTGCGTGGCTGAAATACACGCCGGAGTACGCGGTTCTGGCGGGTGACTATCTGCTGGC
GCGTGTGATGGTGAACCTTTCCGGTCACGGCAACATCAAGCTGGTTCAGTACACGGCGGAAGTGATTTCTGATCTTTTGG
AAGGTGAATGGTTGCAGGACTCTGTGGTGGGCGATTTCTTCGTCACCATGGAACAACTGGATCGCATTCACAATCTGAAG
ACCGCAAGTCTGTTTAAGTGGTGCATCCGTGCGCCGTTCATCGCGCAAGAACGCTACGACGAGGAACTGCACAATATTCT
TTCTGAAATGGGCACGCTGCTGGGTCAGTTGTTCCAGCGCAGTGATGACCTTCTGGATTACGACATCCGTAACGACGAAG
GCAAAGCGATCCTGGGTGACTTGAAATCCGGTTATCTGAATTCCTTCGGGGCGTTCATGTGTGCCGGGGCCACTCGCCAT
GAAATCGACCATATCGTGAAATCCAAAACTCTGGAGCAGTACTACGAAAACGTCGGCGGCAAAGCGCGCTTTGACCAGAA
ACTGGCGGAATTCGACGAGATCAACCGCGGCCTGATCAAAATGTATGATCATCATCTGGAGCGCCTGAAGTCCCTGTTGA
AGCCGGGCGAAGAAAAGCTGATTGATCAACTGCGCCCGCTGACCGAGATTTTGTACTGGAGAAGGAAGCCTTCGTGA

Upstream 100 bases:

>100_bases
TTATTCATAATGTCTCGGACTTTACGGCCTTGAAGCCCTTGACAGCAAGCAGATAAATCGCGAAGTTAGCGGGACTATTC
GACCTCTTCAGGAGTTAAAA

Downstream 100 bases:

>100_bases
GTGAACTGATCACGCTTTCCAAAAAAGACGCCCAGTTTGAATCCTATTTGCTGGGGACGTTCTCCAAAACACACCGGGCT
CTGCCGCAGCAGACCCTGAA

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQRSVIDLKVYDPKDFPAYLPKLNKLYEDLFSGGKGFRAKLIRMMSAHLGIEPKTELLLSQTIEFIHNASLLHDDLIDRS
HLRRGKTAAWLKYTPEYAVLAGDYLLARVMVNLSGHGNIKLVQYTAEVISDLLEGEWLQDSVVGDFFVTMEQLDRIHNLK
TASLFKWCIRAPFIAQERYDEELHNILSEMGTLLGQLFQRSDDLLDYDIRNDEGKAILGDLKSGYLNSFGAFMCAGATRH
EIDHIVKSKTLEQYYENVGGKARFDQKLAEFDEINRGLIKMYDHHLERLKSLLKPGEEKLIDQLRPLTEILYWRRKPS

Sequences:

>Translated_318_residues
MQRSVIDLKVYDPKDFPAYLPKLNKLYEDLFSGGKGFRAKLIRMMSAHLGIEPKTELLLSQTIEFIHNASLLHDDLIDRS
HLRRGKTAAWLKYTPEYAVLAGDYLLARVMVNLSGHGNIKLVQYTAEVISDLLEGEWLQDSVVGDFFVTMEQLDRIHNLK
TASLFKWCIRAPFIAQERYDEELHNILSEMGTLLGQLFQRSDDLLDYDIRNDEGKAILGDLKSGYLNSFGAFMCAGATRH
EIDHIVKSKTLEQYYENVGGKARFDQKLAEFDEINRGLIKMYDHHLERLKSLLKPGEEKLIDQLRPLTEILYWRRKPS
>Mature_318_residues
MQRSVIDLKVYDPKDFPAYLPKLNKLYEDLFSGGKGFRAKLIRMMSAHLGIEPKTELLLSQTIEFIHNASLLHDDLIDRS
HLRRGKTAAWLKYTPEYAVLAGDYLLARVMVNLSGHGNIKLVQYTAEVISDLLEGEWLQDSVVGDFFVTMEQLDRIHNLK
TASLFKWCIRAPFIAQERYDEELHNILSEMGTLLGQLFQRSDDLLDYDIRNDEGKAILGDLKSGYLNSFGAFMCAGATRH
EIDHIVKSKTLEQYYENVGGKARFDQKLAEFDEINRGLIKMYDHHLERLKSLLKPGEEKLIDQLRPLTEILYWRRKPS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=310, Percent_Identity=29.3548387096774, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1789578, Length=204, Percent_Identity=34.3137254901961, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17505681, Length=195, Percent_Identity=34.3589743589744, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319475, Length=228, Percent_Identity=32.4561403508772, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24651612, Length=232, Percent_Identity=30.1724137931034, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36710; Mature: 36710

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSVIDLKVYDPKDFPAYLPKLNKLYEDLFSGGKGFRAKLIRMMSAHLGIEPKTELLLS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QTIEFIHNASLLHDDLIDRSHLRRGKTAAWLKYTPEYAVLAGDYLLARVMVNLSGHGNIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEE
LVQYTAEVISDLLEGEWLQDSVVGDFFVTMEQLDRIHNLKTASLFKWCIRAPFIAQERYD
EHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
EELHNILSEMGTLLGQLFQRSDDLLDYDIRNDEGKAILGDLKSGYLNSFGAFMCAGATRH
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
EIDHIVKSKTLEQYYENVGGKARFDQKLAEFDEINRGLIKMYDHHLERLKSLLKPGEEKL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IDQLRPLTEILYWRRKPS
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQRSVIDLKVYDPKDFPAYLPKLNKLYEDLFSGGKGFRAKLIRMMSAHLGIEPKTELLLS
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
QTIEFIHNASLLHDDLIDRSHLRRGKTAAWLKYTPEYAVLAGDYLLARVMVNLSGHGNIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEE
LVQYTAEVISDLLEGEWLQDSVVGDFFVTMEQLDRIHNLKTASLFKWCIRAPFIAQERYD
EHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHH
EELHNILSEMGTLLGQLFQRSDDLLDYDIRNDEGKAILGDLKSGYLNSFGAFMCAGATRH
HHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHH
EIDHIVKSKTLEQYYENVGGKARFDQKLAEFDEINRGLIKMYDHHLERLKSLLKPGEEKL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IDQLRPLTEILYWRRKPS
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]