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The map label for this gene is truA

Identifier: 42522098

GI number: 42522098

Start: 459344

End: 460132

Strand: Direct

Name: truA

Synonym: Bd0491

Alternate gene names: 42522098

Gene position: 459344-460132 (Clockwise)

Preceding gene: 42522097

Following gene: 42522099

Centisome position: 12.14

GC content: 50.95

Gene sequence:

>789_bases
ATGACTACTAAAGTGCGCTTTACCGTTGCTTATGACGGAACAGGATTCTGCGGCTGGCAGAAACAAAAACCCGAGGACCA
GATTTCGGTCGCTCAGGTGATCGAAGAGGCCCTGTCCAAGGTCTTCAATGAAAAAATCACCCTGTTTGCTTCCGGGCGCA
CAGACGCCGGGGTTCATGCCCTGAACCAGGTCTGCCACTTTTCTACGCATCGCAAGATTGATCCGAACAAGAAATGGGAC
CTGTGCTGGGCTTTGAATTCCCACCTGCCCCCAAGTATTGTGGCCAAAAAAGCCTGGATTGCTCCGGATGATTTCCATGC
GACCTTGTCGGCGACTCATAAGACCTATCGGTATCTTATAGTGAACAAGCCCCGTCCCAGCGCCCATTTAAACCGCTATG
CGGACTGGGTGCGTCTGCCCATCGATATCGAACATTTGCAGGAAAGTTCAAAATATCTCTTGGGAAATCAAGACTTTAAG
AGCTTTCAATCGGTCGGAACCCCGGTTCCTGATACGGTTCGTGAGATCTATAAAGCAGACTGGGAGTGGCGCAAACCGGG
TGTGATGCAGTTCACGATCACCGGCAGCGGCTTTTTAAAGCAGATGGTTCGAAATATTGTGGGGACCTCATTGTTTTTGG
AGCGCAAGGGCCTGGATCCATCTAAAATGCAGGAGATCATTGCGGCTCAGGACCGCATGAAAGCCGGACCGCCGGCCCCC
GCACAGGGCCTGTATTTGATGAAGGTTTATTATCCTCAGGACCTTGACAATAGGTGCCTAGAACTTTAA

Upstream 100 bases:

>100_bases
TTCGGCGGCAAGGTCGTCACCTGCCAGGGAGCTTTGTTCTAGGCTGTTAAAAGTTCCCTCCATCTTGCGGCCCTTCGGGG
AAACTGGTAGGTTGCCGCGC

Downstream 100 bases:

>100_bases
AACATCCCTTCCCGCAAAATAGTGTAAGAGCCCGTTGTTAAACTCAAACATATTTTTGCCGAGCAGACGGACGGTAAAAC
CGGCTGTTCAAAATGAAACT

Product: pseudouridylate synthase I

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTTKVRFTVAYDGTGFCGWQKQKPEDQISVAQVIEEALSKVFNEKITLFASGRTDAGVHALNQVCHFSTHRKIDPNKKWD
LCWALNSHLPPSIVAKKAWIAPDDFHATLSATHKTYRYLIVNKPRPSAHLNRYADWVRLPIDIEHLQESSKYLLGNQDFK
SFQSVGTPVPDTVREIYKADWEWRKPGVMQFTITGSGFLKQMVRNIVGTSLFLERKGLDPSKMQEIIAAQDRMKAGPPAP
AQGLYLMKVYYPQDLDNRCLEL

Sequences:

>Translated_262_residues
MTTKVRFTVAYDGTGFCGWQKQKPEDQISVAQVIEEALSKVFNEKITLFASGRTDAGVHALNQVCHFSTHRKIDPNKKWD
LCWALNSHLPPSIVAKKAWIAPDDFHATLSATHKTYRYLIVNKPRPSAHLNRYADWVRLPIDIEHLQESSKYLLGNQDFK
SFQSVGTPVPDTVREIYKADWEWRKPGVMQFTITGSGFLKQMVRNIVGTSLFLERKGLDPSKMQEIIAAQDRMKAGPPAP
AQGLYLMKVYYPQDLDNRCLEL
>Mature_261_residues
TTKVRFTVAYDGTGFCGWQKQKPEDQISVAQVIEEALSKVFNEKITLFASGRTDAGVHALNQVCHFSTHRKIDPNKKWDL
CWALNSHLPPSIVAKKAWIAPDDFHATLSATHKTYRYLIVNKPRPSAHLNRYADWVRLPIDIEHLQESSKYLLGNQDFKS
FQSVGTPVPDTVREIYKADWEWRKPGVMQFTITGSGFLKQMVRNIVGTSLFLERKGLDPSKMQEIIAAQDRMKAGPPAPA
QGLYLMKVYYPQDLDNRCLEL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=31.0469314079422, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1788657, Length=253, Percent_Identity=37.5494071146245, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BDEBA (Q6MQH6)

Other databases:

- EMBL:   BX842647
- RefSeq:   NP_967478.1
- ProteinModelPortal:   Q6MQH6
- SMR:   Q6MQH6
- GeneID:   2733092
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0491
- NMPDR:   fig|264462.1.peg.448
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q6MQH6
- BioCyc:   BBAC264462:BD0491-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29867; Mature: 29735

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: ACT_SITE 55-55

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKVRFTVAYDGTGFCGWQKQKPEDQISVAQVIEEALSKVFNEKITLFASGRTDAGVHA
CCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
LNQVCHFSTHRKIDPNKKWDLCWALNSHLPPSIVAKKAWIAPDDFHATLSATHKTYRYLI
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCEEEEEE
VNKPRPSAHLNRYADWVRLPIDIEHLQESSKYLLGNQDFKSFQSVGTPVPDTVREIYKAD
EECCCCHHHHHHHHHHEECCCCHHHHHCCCCEECCCCHHHHHHHCCCCCCHHHHHHHHCC
WEWRKPGVMQFTITGSGFLKQMVRNIVGTSLFLERKGLDPSKMQEIIAAQDRMKAGPPAP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCC
AQGLYLMKVYYPQDLDNRCLEL
CCCEEEEEEECCCCCCHHHHCC
>Mature Secondary Structure 
TTKVRFTVAYDGTGFCGWQKQKPEDQISVAQVIEEALSKVFNEKITLFASGRTDAGVHA
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
LNQVCHFSTHRKIDPNKKWDLCWALNSHLPPSIVAKKAWIAPDDFHATLSATHKTYRYLI
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHCCCEEEEEE
VNKPRPSAHLNRYADWVRLPIDIEHLQESSKYLLGNQDFKSFQSVGTPVPDTVREIYKAD
EECCCCHHHHHHHHHHEECCCCHHHHHCCCCEECCCCHHHHHHHCCCCCCHHHHHHHHCC
WEWRKPGVMQFTITGSGFLKQMVRNIVGTSLFLERKGLDPSKMQEIIAAQDRMKAGPPAP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCC
AQGLYLMKVYYPQDLDNRCLEL
CCCEEEEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA