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The map label for this gene is tpx [H]

Identifier: 42521937

GI number: 42521937

Start: 302192

End: 302689

Strand: Direct

Name: tpx [H]

Synonym: Bd0315

Alternate gene names: 42521937

Gene position: 302192-302689 (Clockwise)

Preceding gene: 42521936

Following gene: 42521938

Centisome position: 7.99

GC content: 50.8

Gene sequence:

>498_bases
ATGGCATCGATCACACTCAAAGGCAATCCCGTTAACACTTCCGGCACTCTTCCTGAAGCGGGCACTGCCGCCAAAGACTT
CAAATTTGTGCGCAACGATCTTTCTGAAGTCTCTTTGCACACCTTCGCAGGCAAAAAGAAAGTTTTGAACATCTTCCCAA
GCGTGGACACCGGCACTTGTGCTGCTTCTGTTCGTCGTTTCAATCAGGAAGCCTCCAAACTGGATAACACGGTTGTTCTG
AACATCTCTGCGGACCTGCCGTTTGCCCAAGCCCGTTTCTGTGGCGCTGAAGGCATCAAGAACTGCGAGACTGTTTCCAC
TTTCCGCAGCTCTTTTGGCAAGGACTGGGGCGTTCAGATCGTGGATTCTCCGCTGGCGGGTCTTCTTTCCCGTGCGGTGG
TGACTCTTTCTGCAGATAACAAGATTTTGTATGTTGAGCAGGTGTCCGAGATCGTCAATGAGCCAAATTACGAAGCGGCT
TTGGCGTCTTTGAAGTAA

Upstream 100 bases:

>100_bases
ATCGTCAGCTGGAGCCAGAATCTGTCTATTTGCAAGTAGATCGTTTTGATCTTCACCGGATCGGTGCTATGCTGGGTTCG
CTTAAACAAAAGGAGCATCC

Downstream 100 bases:

>100_bases
TCAGGTCTGTTATAATTGCAAAGCGGCTTTGGGCGAAGGCTCAAAGCCGCTTTTTTTTGTCTCTTTTCAACGGGTTGTGG
TACACCTGCCCGCTATGAAA

Product: thiol peroxidase

Products: oxidized thioredoxin; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MASITLKGNPVNTSGTLPEAGTAAKDFKFVRNDLSEVSLHTFAGKKKVLNIFPSVDTGTCAASVRRFNQEASKLDNTVVL
NISADLPFAQARFCGAEGIKNCETVSTFRSSFGKDWGVQIVDSPLAGLLSRAVVTLSADNKILYVEQVSEIVNEPNYEAA
LASLK

Sequences:

>Translated_165_residues
MASITLKGNPVNTSGTLPEAGTAAKDFKFVRNDLSEVSLHTFAGKKKVLNIFPSVDTGTCAASVRRFNQEASKLDNTVVL
NISADLPFAQARFCGAEGIKNCETVSTFRSSFGKDWGVQIVDSPLAGLLSRAVVTLSADNKILYVEQVSEIVNEPNYEAA
LASLK
>Mature_164_residues
ASITLKGNPVNTSGTLPEAGTAAKDFKFVRNDLSEVSLHTFAGKKKVLNIFPSVDTGTCAASVRRFNQEASKLDNTVVLN
ISADLPFAQARFCGAEGIKNCETVSTFRSSFGKDWGVQIVDSPLAGLLSRAVVTLSADNKILYVEQVSEIVNEPNYEAAL
ASLK

Specific function: Has antioxidant activity. Could remove peroxides or H(2)O(2) [H]

COG id: COG2077

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1787584, Length=164, Percent_Identity=57.3170731707317, Blast_Score=199, Evalue=7e-53,

Paralogues:

None

Copy number: 2480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018219
- InterPro:   IPR002065
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: 1.11.1.- [C]

Molecular weight: Translated: 17644; Mature: 17513

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01265 TPX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASITLKGNPVNTSGTLPEAGTAAKDFKFVRNDLSEVSLHTFAGKKKVLNIFPSVDTGTC
CCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHH
AASVRRFNQEASKLDNTVVLNISADLPFAQARFCGAEGIKNCETVSTFRSSFGKDWGVQI
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEE
VDSPLAGLLSRAVVTLSADNKILYVEQVSEIVNEPNYEAALASLK
ECCHHHHHHHHHEEEEECCCCEEEHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
ASITLKGNPVNTSGTLPEAGTAAKDFKFVRNDLSEVSLHTFAGKKKVLNIFPSVDTGTC
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHH
AASVRRFNQEASKLDNTVVLNISADLPFAQARFCGAEGIKNCETVSTFRSSFGKDWGVQI
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEE
VDSPLAGLLSRAVVTLSADNKILYVEQVSEIVNEPNYEAALASLK
ECCHHHHHHHHHEEEEECCCCEEEHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced thioredoxin; H2O2 [C]

Specific reaction: reduced thioredoxin + H2O2 = oxidized thioredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]