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The map label for this gene is cwlM

Identifier: 41410439

GI number: 41410439

Start: 4821429

End: 4822649

Strand: Direct

Name: cwlM

Synonym: MAP4341

Alternate gene names: 41410439

Gene position: 4821429-4822649 (Clockwise)

Preceding gene: 41410438

Following gene: NA

Centisome position: 99.83

GC content: 66.67

Gene sequence:

>1221_bases
ATGTCGAGTCCGCGCCGCGAATACGGCGACGCGCTGCGCTGTGGTGACCGCAGCGCTGCTGTGACCGAGATCCGGGCCAC
GCTCGCCTCGTTAGGGCTGCTGGCCAGCGCCGATGAGGATCTCAGCACCGGCCGGCACGTGGCCCTGGAACTCTTCGACG
CCGAGCTCGACCAGGCCGTCCGCGCCTTTCAACAGCATCGCGGCCTGCTGGTGGACGGCATCGTCGGGGAAGCGACCTAC
CGGGCGCTCAAGGAGGCGTCCTACCGGCTCGGCGCCCGCACGCTGTATCACCAATTCGGCGCGCCGCTTTACGGCGACGA
CGTCGCGACGCTGCAGGCCCGGCTGCAGGATCTCGGTTTCTACACCGGCCTGGTCGACGGCTACTTCGGGTTGCAGACCC
ACAACGCTTTGATGTCGTATCAGCGCGAGTACGGGTTGTCCGCCGACGGCATCTGCGGCCCGGAAACGTTGCGCTCGTTG
TATTTTCTGAGTTCGCGGGTCACCGGCGGATCGCCGCACGCGCTGCGCGAGGAGGAGCTGGTCCGCCGATCGGGCCCCAA
ACTGTCCGGCAAGCGAATCATCATCGACCCGGGCCGCGGCGGCGCCGACCACGGCATGATCATGCAGGGTCCGTCCGGCC
CGATCAGCGAAGCAGATGTGTTGTGGGACTTGGCAAGCCGGCTCGAGGGCCGGATGACCGCGATCGGCATGGAGACCTTC
CTGTCGCGGCCGGTCAACCGCAGCCCGCTGGATGCCGAACGCGCGGCCACCGCCAACAATGTCGGCGCCGACCTGATGAT
CAGCCTGCGCTGCGAAGCTCAAAAAAGCCCCGCCGCCAACGGGGTGGCATCCTTTCACTTCGGCAACTCGCACGGCTCGG
TATCCACCATCGGGCGCAACCTCGCCGACTTCATCCAACGAGAAGTGGTGGCCCGCACCGGTTTACGGGACTGCCGAACG
CACGGCCGGACGTGGGATCTGCTGCGGCTGACCCGCATGCCCACGGTTCAGGTCGACGTCGGCTACGTCACCAACCCGCA
CGACCGCGCGATGCTGGTGTCCACCCAGACCCGCGATGCGATCGCCGAGGGAATCCTCGCCGCGGTCAAGCGGCTCTACC
TGTTGGGCAAAAACGACCGTCCCACAGGCACATTCACTTTCGCCGAGTTACTGGCCCACGAATTGTCGGTGGAGCGCACC
AGCCAGATGGGCGGTTCCTGA

Upstream 100 bases:

>100_bases
GATGGCCTGGGGTTTTCCCGAATTCACGAAGCGTCTGCGACAATACGGTTAGCTGTTGCCGCTATGCGAGCCAGTCAGTT
TGTCCCGGAGGGCCCTTGGT

Downstream 100 bases:

>100_bases
ACCGCACCGGCCGCTAGTCGGGCGGGGCGGTTTTCAATGCGTTCGCCGGCGTGGACGCGGCCCCGACCGGCTGCTCGAGG
CGGGCGCTCTCCAGCAGACG

Product: CwlM

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSSPRREYGDALRCGDRSAAVTEIRATLASLGLLASADEDLSTGRHVALELFDAELDQAVRAFQQHRGLLVDGIVGEATY
RALKEASYRLGARTLYHQFGAPLYGDDVATLQARLQDLGFYTGLVDGYFGLQTHNALMSYQREYGLSADGICGPETLRSL
YFLSSRVTGGSPHALREEELVRRSGPKLSGKRIIIDPGRGGADHGMIMQGPSGPISEADVLWDLASRLEGRMTAIGMETF
LSRPVNRSPLDAERAATANNVGADLMISLRCEAQKSPAANGVASFHFGNSHGSVSTIGRNLADFIQREVVARTGLRDCRT
HGRTWDLLRLTRMPTVQVDVGYVTNPHDRAMLVSTQTRDAIAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVERT
SQMGGS

Sequences:

>Translated_406_residues
MSSPRREYGDALRCGDRSAAVTEIRATLASLGLLASADEDLSTGRHVALELFDAELDQAVRAFQQHRGLLVDGIVGEATY
RALKEASYRLGARTLYHQFGAPLYGDDVATLQARLQDLGFYTGLVDGYFGLQTHNALMSYQREYGLSADGICGPETLRSL
YFLSSRVTGGSPHALREEELVRRSGPKLSGKRIIIDPGRGGADHGMIMQGPSGPISEADVLWDLASRLEGRMTAIGMETF
LSRPVNRSPLDAERAATANNVGADLMISLRCEAQKSPAANGVASFHFGNSHGSVSTIGRNLADFIQREVVARTGLRDCRT
HGRTWDLLRLTRMPTVQVDVGYVTNPHDRAMLVSTQTRDAIAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVERT
SQMGGS
>Mature_405_residues
SSPRREYGDALRCGDRSAAVTEIRATLASLGLLASADEDLSTGRHVALELFDAELDQAVRAFQQHRGLLVDGIVGEATYR
ALKEASYRLGARTLYHQFGAPLYGDDVATLQARLQDLGFYTGLVDGYFGLQTHNALMSYQREYGLSADGICGPETLRSLY
FLSSRVTGGSPHALREEELVRRSGPKLSGKRIIIDPGRGGADHGMIMQGPSGPISEADVLWDLASRLEGRMTAIGMETFL
SRPVNRSPLDAERAATANNVGADLMISLRCEAQKSPAANGVASFHFGNSHGSVSTIGRNLADFIQREVVARTGLRDCRTH
GRTWDLLRLTRMPTVQVDVGYVTNPHDRAMLVSTQTRDAIAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVERTS
QMGGS

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44096; Mature: 43965

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRREYGDALRCGDRSAAVTEIRATLASLGLLASADEDLSTGRHVALELFDAELDQAV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEHHHHHHHHHHHH
RAFQQHRGLLVDGIVGEATYRALKEASYRLGARTLYHQFGAPLYGDDVATLQARLQDLGF
HHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YTGLVDGYFGLQTHNALMSYQREYGLSADGICGPETLRSLYFLSSRVTGGSPHALREEEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VRRSGPKLSGKRIIIDPGRGGADHGMIMQGPSGPISEADVLWDLASRLEGRMTAIGMETF
HHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHEEHHHH
LSRPVNRSPLDAERAATANNVGADLMISLRCEAQKSPAANGVASFHFGNSHGSVSTIGRN
HCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHH
LADFIQREVVARTGLRDCRTHGRTWDLLRLTRMPTVQVDVGYVTNPHDRAMLVSTQTRDA
HHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHCCCEEEEEECEEECCCCCEEEEECCHHHH
IAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVERTSQMGGS
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSPRREYGDALRCGDRSAAVTEIRATLASLGLLASADEDLSTGRHVALELFDAELDQAV
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCEEHHHHHHHHHHHH
RAFQQHRGLLVDGIVGEATYRALKEASYRLGARTLYHQFGAPLYGDDVATLQARLQDLGF
HHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YTGLVDGYFGLQTHNALMSYQREYGLSADGICGPETLRSLYFLSSRVTGGSPHALREEEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VRRSGPKLSGKRIIIDPGRGGADHGMIMQGPSGPISEADVLWDLASRLEGRMTAIGMETF
HHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHEEHHHH
LSRPVNRSPLDAERAATANNVGADLMISLRCEAQKSPAANGVASFHFGNSHGSVSTIGRN
HCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHH
LADFIQREVVARTGLRDCRTHGRTWDLLRLTRMPTVQVDVGYVTNPHDRAMLVSTQTRDA
HHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHCCCEEEEEECEEECCCCCEEEEECCHHHH
IAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVERTSQMGGS
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]