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The map label for this gene is truA

Identifier: 41410333

GI number: 41410333

Start: 4702099

End: 4702854

Strand: Direct

Name: truA

Synonym: MAP4235

Alternate gene names: 41410333

Gene position: 4702099-4702854 (Clockwise)

Preceding gene: 41410332

Following gene: 41410336

Centisome position: 97.36

GC content: 73.15

Gene sequence:

>756_bases
GTGGCCGGGGTGCTCGACGAGGCGCTGACGACCGTCTTCCGCACGCCGGTGCGGTTGCGCGCCGCGGGCCGTACCGACGC
GGGGGTGCACGCCACCGGGCAGGTCGCTCATCTCGACGTGCCGGGCACCGCGCTGCCGAACGCCTATCCGCGCGCACCGC
ACGTCGGCGAAGCCGAATTCGGGCCGCTGCTGCGCCGGCTGGGCCGGTTCCTGCCCACCGACGTCCGCGTCGTCGACATC
ACCCGCGCGCCAGCGGGTTTCGACGCCCGGTTCTCGGCGCTGCGGCGCCACTACGTCTACCGGCTCTCGACGGCCCCGTG
GGGTGTGCTGCCGCAGCAGGCTCGCTACGTCACCGCCTGGCCCCGCCCCCTGGATGTCGAGGCGATGGCCGCCGCGTCGC
GAGACCTGTTGGGGCTGCACGACTTCGCCGCGTTCTGCCGTCACCGCGAGAACGCCACCACCATCCGCGACCTGCAGCGC
CTGGACTGGACCCGCGACGGCGACCTGATCACCGCGAGGGTCAGCGCCGACGCGTTCTGCTGGTCGATGGTGCGCTCCCT
GGTGGGGGCGCTGCTGGCGGTCGGGGAGAACCGGCGGCCGGTGTCCTGGTGTCGCGAATTGCTCAGCGCCACAAACCGAT
CCAGTGACTACGCCACCGCACCGGCGCACGGGCTGACCCTGGTCGGGGTCGACTACCCGCCGGATGACCAGCTGGCGGCG
CGCAACCTGATCACCCGCGACGTGCGCTCGCGCTAG

Upstream 100 bases:

>100_bases
GGGTCCAGCGAGGATTAGCGAGGACGTCCGTCTGCGGCTCGATATCGCCTATGACGGAACAGGTTTCGCGGGCTGGGCTA
CTCAGTCCGGCCAGCGGACC

Downstream 100 bases:

>100_bases
ACCCGGGTGGCGACGAACCTGGCTGCCTGGTTGGTCAATCCGGGCACATAGCTCAGGTGCGACGCCCACTGGTAACCGCC
CGAGCAGATCGGGTCGCCCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MAGVLDEALTTVFRTPVRLRAAGRTDAGVHATGQVAHLDVPGTALPNAYPRAPHVGEAEFGPLLRRLGRFLPTDVRVVDI
TRAPAGFDARFSALRRHYVYRLSTAPWGVLPQQARYVTAWPRPLDVEAMAAASRDLLGLHDFAAFCRHRENATTIRDLQR
LDWTRDGDLITARVSADAFCWSMVRSLVGALLAVGENRRPVSWCRELLSATNRSSDYATAPAHGLTLVGVDYPPDDQLAA
RNLITRDVRSR

Sequences:

>Translated_251_residues
MAGVLDEALTTVFRTPVRLRAAGRTDAGVHATGQVAHLDVPGTALPNAYPRAPHVGEAEFGPLLRRLGRFLPTDVRVVDI
TRAPAGFDARFSALRRHYVYRLSTAPWGVLPQQARYVTAWPRPLDVEAMAAASRDLLGLHDFAAFCRHRENATTIRDLQR
LDWTRDGDLITARVSADAFCWSMVRSLVGALLAVGENRRPVSWCRELLSATNRSSDYATAPAHGLTLVGVDYPPDDQLAA
RNLITRDVRSR
>Mature_250_residues
AGVLDEALTTVFRTPVRLRAAGRTDAGVHATGQVAHLDVPGTALPNAYPRAPHVGEAEFGPLLRRLGRFLPTDVRVVDIT
RAPAGFDARFSALRRHYVYRLSTAPWGVLPQQARYVTAWPRPLDVEAMAAASRDLLGLHDFAAFCRHRENATTIRDLQRL
DWTRDGDLITARVSADAFCWSMVRSLVGALLAVGENRRPVSWCRELLSATNRSSDYATAPAHGLTLVGVDYPPDDQLAAR
NLITRDVRSR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=238, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788657, Length=236, Percent_Identity=37.7118644067797, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_MYCPA (Q73S41)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_963169.1
- ProteinModelPortal:   Q73S41
- SMR:   Q73S41
- EnsemblBacteria:   EBMYCT00000039971
- GeneID:   2718866
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP4235
- NMPDR:   fig|262316.1.peg.4235
- GeneTree:   EBGT00050000017011
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27674; Mature: 27543

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: ACT_SITE 26-26

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVLDEALTTVFRTPVRLRAAGRTDAGVHATGQVAHLDVPGTALPNAYPRAPHVGEAEF
CCCHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHH
GPLLRRLGRFLPTDVRVVDITRAPAGFDARFSALRRHYVYRLSTAPWGVLPQQARYVTAW
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEC
PRPLDVEAMAAASRDLLGLHDFAAFCRHRENATTIRDLQRLDWTRDGDLITARVSADAFC
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHH
WSMVRSLVGALLAVGENRRPVSWCRELLSATNRSSDYATAPAHGLTLVGVDYPPDDQLAA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
RNLITRDVRSR
HHHHHHHHHCC
>Mature Secondary Structure 
AGVLDEALTTVFRTPVRLRAAGRTDAGVHATGQVAHLDVPGTALPNAYPRAPHVGEAEF
CCHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHH
GPLLRRLGRFLPTDVRVVDITRAPAGFDARFSALRRHYVYRLSTAPWGVLPQQARYVTAW
HHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEC
PRPLDVEAMAAASRDLLGLHDFAAFCRHRENATTIRDLQRLDWTRDGDLITARVSADAFC
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHH
WSMVRSLVGALLAVGENRRPVSWCRELLSATNRSSDYATAPAHGLTLVGVDYPPDDQLAA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
RNLITRDVRSR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA