Click here to switch to the map view.

The map label for this gene is echA5

Identifier: 41410234

GI number: 41410234

Start: 4615039

End: 4615806

Strand: Direct

Name: echA5

Synonym: MAP4136

Alternate gene names: 41410234

Gene position: 4615039-4615806 (Clockwise)

Preceding gene: 41410233

Following gene: 41410237

Centisome position: 95.55

GC content: 71.74

Gene sequence:

>768_bases
ATGAGCGATCCGGTGCGAATCGAACGCAACGGCCCGGTCACCACGGTGATCATCAACCGGCCGGCGGCGCGCAACGCGGT
CAACGGCCCGACGGCGGCCGCGCTGTACGCGGCGTTCGAGGAGTTCGACCGCGACGACTCCGCGTCGGTGGCCGTGCTGT
GGGGCGAGGGCGGAACATTCTGCGCCGGAGCAGATTTGAAGGCGTTCGGCACGCCCGAGGCCAACGCCGTGCACCGGAGT
GGCCCCGGCCCGATGGGCCCGACCCGGATGGTGCTGTCCAAACCGGTCATCGCCGCGGTCAGCGGCTACGCCGTCGCGGG
TGGTCTGGAGCTGGCCATCTGGTGTGATCTGCGGGTGGTCGAGCAGGACGCCGTGTTCGGGGTGTTCTGCCGGCGCTGGG
GGGTCCCGCTGATCGACGGCGGCACGGTGCGGCTGCCGCGGCTGATCGGGCACAGCCGCGCGATGGACATGATCCTCACC
GGCCGCGCCGTGGCCGCCGACGAAGCCCAGGCCATCGGCTTGGCGAATCGCGTTGTCCCCAAAGGTCAATCGCGCCGGGC
GGCCGAGGAATTGGCGGCGCAACTGGCCGCGCTGCCGCAGCAGTGCCTGCGCTCGGACCGGCTCTCGGCGCTACATCAGT
GGGGCGCAACGGAATCCGAGGCACTCGACTTCGAATTCGCCAGCATCTCCCGGGTGGCCGCCGAGGCCAACGAGGGCGCC
GGCCGGTTCGCCGCGGGCGCGGGACGGCACGGCGCCTCCGCGGTTTGA

Upstream 100 bases:

>100_bases
TGCTGCCCCCGGACTGGCCCGGCACCCGGCTGCGGGCGGCCTACCACGACTTCGCCGCGGAGCTGATGGCGCGGCGCGAC
CCACTATTTGCGGAGGCGAC

Downstream 100 bases:

>100_bases
GGGTCAGCCGCCCTTGTTGATGGTGTTGCTCTGGCCGATCTTGTCGACCTTGGGGTCACCGTCCTTGTAGGTGATCGTGT
TGTTCATCCCCAGCACGGTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSDPVRIERNGPVTTVIINRPAARNAVNGPTAAALYAAFEEFDRDDSASVAVLWGEGGTFCAGADLKAFGTPEANAVHRS
GPGPMGPTRMVLSKPVIAAVSGYAVAGGLELAIWCDLRVVEQDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILT
GRAVAADEAQAIGLANRVVPKGQSRRAAEELAAQLAALPQQCLRSDRLSALHQWGATESEALDFEFASISRVAAEANEGA
GRFAAGAGRHGASAV

Sequences:

>Translated_255_residues
MSDPVRIERNGPVTTVIINRPAARNAVNGPTAAALYAAFEEFDRDDSASVAVLWGEGGTFCAGADLKAFGTPEANAVHRS
GPGPMGPTRMVLSKPVIAAVSGYAVAGGLELAIWCDLRVVEQDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILT
GRAVAADEAQAIGLANRVVPKGQSRRAAEELAAQLAALPQQCLRSDRLSALHQWGATESEALDFEFASISRVAAEANEGA
GRFAAGAGRHGASAV
>Mature_254_residues
SDPVRIERNGPVTTVIINRPAARNAVNGPTAAALYAAFEEFDRDDSASVAVLWGEGGTFCAGADLKAFGTPEANAVHRSG
PGPMGPTRMVLSKPVIAAVSGYAVAGGLELAIWCDLRVVEQDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILTG
RAVAADEAQAIGLANRVVPKGQSRRAAEELAAQLAALPQQCLRSDRLSALHQWGATESEALDFEFASISRVAAEANEGAG
RFAAGAGRHGASAV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=172, Percent_Identity=35.4651162790698, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=32.15859030837, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI20127408, Length=194, Percent_Identity=31.4432989690722, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=33.1491712707182, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=203, Percent_Identity=34.9753694581281, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI31542718, Length=196, Percent_Identity=26.0204081632653, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI221142681, Length=197, Percent_Identity=37.5634517766497, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787659, Length=196, Percent_Identity=36.2244897959184, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=34.7368421052632, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=28.8372093023256, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790281, Length=245, Percent_Identity=28.1632653061224, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787660, Length=247, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=242, Percent_Identity=48.7603305785124, Blast_Score=226, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI25145438, Length=239, Percent_Identity=32.6359832635983, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=32.2222222222222, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=234, Percent_Identity=32.9059829059829, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=29.8076923076923, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=188, Percent_Identity=33.5106382978723, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=34.7593582887701, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=229, Percent_Identity=27.0742358078603, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=34.5744680851064, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=199, Percent_Identity=34.6733668341709, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=204, Percent_Identity=28.921568627451, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=195, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357171, Length=209, Percent_Identity=48.3253588516746, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24650670, Length=252, Percent_Identity=41.6666666666667, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI20129971, Length=214, Percent_Identity=34.1121495327103, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653477, Length=214, Percent_Identity=34.1121495327103, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653139, Length=197, Percent_Identity=34.5177664974619, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24583077, Length=194, Percent_Identity=32.9896907216495, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583079, Length=194, Percent_Identity=32.9896907216495, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921000, Length=191, Percent_Identity=32.4607329842932, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922422, Length=230, Percent_Identity=24.7826086956522, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26600; Mature: 26469

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPVRIERNGPVTTVIINRPAARNAVNGPTAAALYAAFEEFDRDDSASVAVLWGEGGTF
CCCCEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
CAGADLKAFGTPEANAVHRSGPGPMGPTRMVLSKPVIAAVSGYAVAGGLELAIWCDLRVV
EECCCCEECCCCCCCCEECCCCCCCCCHHHHHHCCHHHHHCCEEEECCEEEEEEEEEEEE
EQDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILTGRAVAADEAQAIGLANRVVP
CCHHHHHHHHHHCCCCEECCCCEEHHHHHCCHHHHHHEEECCCCCCCCHHHHHHHHHCCC
KGQSRRAAEELAAQLAALPQQCLRSDRLSALHQWGATESEALDFEFASISRVAAEANEGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEHHHHHHHHHHHCCCCC
GRFAAGAGRHGASAV
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDPVRIERNGPVTTVIINRPAARNAVNGPTAAALYAAFEEFDRDDSASVAVLWGEGGTF
CCCEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
CAGADLKAFGTPEANAVHRSGPGPMGPTRMVLSKPVIAAVSGYAVAGGLELAIWCDLRVV
EECCCCEECCCCCCCCEECCCCCCCCCHHHHHHCCHHHHHCCEEEECCEEEEEEEEEEEE
EQDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILTGRAVAADEAQAIGLANRVVP
CCHHHHHHHHHHCCCCEECCCCEEHHHHHCCHHHHHHEEECCCCCCCCHHHHHHHHHCCC
KGQSRRAAEELAAQLAALPQQCLRSDRLSALHQWGATESEALDFEFASISRVAAEANEGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCEEHHHHHHHHHHHCCCCC
GRFAAGAGRHGASAV
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA