Click here to switch to the map view.

The map label for this gene is menD

Identifier: 41410150

GI number: 41410150

Start: 4512395

End: 4514032

Strand: Direct

Name: menD

Synonym: MAP4052

Alternate gene names: 41410150

Gene position: 4512395-4514032 (Clockwise)

Preceding gene: 41410149

Following gene: 41410151

Centisome position: 93.43

GC content: 75.03

Gene sequence:

>1638_bases
GTGAACCCCTCGACGACCCAGGCCCGCGTCGTCGTCGACGAGCTGATCCGCGGCGGTGTCCGCGATGTGGTGCTGTGCCC
CGGTTCCCGCAACGCCCCGCTGGCCTTCGCGCTGGCCGACGCCGACCGCGCCGGCCGGATCCGGCTGCACGTGCGCATCG
ACGAGCGCACCGCCGGCTATCTGGCCATCGGCCTGGCCATCGCGGCCGGCGCTCCGGTGTGCGTCGCGATGACCTCGGGC
ACCGCGGTGGCCAACCTGGGCCCGGCCGTGGTGGAGGCCAACTACGCGCGGGTGCCGCTGATCGTGCTGAGCGCCAACCG
GCCCTACGAACTGCTGGGCACCGGCGCCAACCAGACCATGGAGCAGCTGGGCTACTTCGGCACCCAGGTCCGGGCCGCCA
TCAGCCTGGGCCTGGCCGAGGACGCCCCCGAGCGGCTGGACGCGCTCAACGCCACCTGGCGCTCGGCCACCTGCCGGGTG
CTGGCGGCGGCCACCGGATCCCGCACCGCCAACGCCGGCCCGGTGCAGTTCGACATCCCGCTGCGTGAGCCGCTGGTGCC
CGATCCCGAGCCGCACGGCGCCCCGACCCCGGCCGGCCGCCCGGGCGGCCGGCCGTGGACCTATACCCCGCCGGTCAGCT
TCGACCAGCCGCTGGACATCGATCTGTCACCGGACACCGTCGTCATCGCCGGCCACGGCGCCGGCACGCACCCCAACCTT
GCCCAGCTGCCGACCGTGGCCGAGCCGACGGCACCCGCCCCGGACAACCCGCTGCACCCGCTGGCGCTGCGGTTGCTGCG
CCCCAAGCAGGTGATCATGCTGGGCCGGCCCACCCTGCACCGGCCGGTGTCGGCGCTGCTGGCCGACCCGCAGGTGCCGG
TGTACGCGCTGACCACCGGTCCGCGCTGGCCCGACGTGTCGGGCAACTCGCAGGCCACCGGGACCCGGGCGGTCACCACC
GGGGCGCCGAGCCCGGCCTGGCTGCACCGCTGCGAACAGGCCAACCGGCACGCCGTGGCCGCCGTGCGCGGCCAGCTGGC
GGCGCACCCGCTGACCACCGGCCTGCACGTGGCGGCGGCGGTGGCCGACGCGCTGCGGCCCGGCGACCAGCTGGTGCTCG
GGGCGTCCAACCCGGTCCGCGACGCGGCGTTGGTCGGGCTGGACAGCCACCACATCCGGGTGCGCTCCAACCGCGGGGTG
GCCGGCATCGACGGCACGGTGTCGACGGCGATCGGCGCGGCCCTGGCCCACGAGGCGGCGCACGACGGGCGCACCGTCGC
GCTGATCGGCGACCTGACGTTCGTGCACGACAGCTCCGGGCTGCTGATCGGGCCGACCGAGCCCACCCCACGCCGGCTGA
CGATCGTGGTGTCCAACGACAACGGCGGCGGCATCTTCGAGCTGCTAGAGCAGGGCGACCCGCGGTTCTCCGACGTGTCG
TCGCGGGTCTTCGGCACTCCGCACGACGTCGACGTGGGGGCGCTGTGCCGGGCGTATCACGTCGAGAGCGCCCAGATCGA
GGTCGGCGAATTGGCCGCCGCCCTCGACGAACCCGGGCCCGGGATGCGGGTGCTGGAGGTCAAGGCCGACCGCTCGTCGC
TGCGGCAACTGCACGCCGCCATCAAGGCGGCGCTGTGA

Upstream 100 bases:

>100_bases
ACTAGGCGCCCAACGTCGACTTGTGCGGCGAGATCGCGCCGGCGGGCCGCAACAACTCCACGTTGGGCGCCTCATCGACC
GGCTGTGACACGCTGTATGG

Downstream 100 bases:

>100_bases
CGATATCCCCAAAAGCGTTGCTGCACATCCTGATTCACGGACGCAGCGACCCGCCGGAGACAACCCGGGGCCGGATCGCG
TTGCGCTGGGCCAGGATCGC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALADADRAGRIRLHVRIDERTAGYLAIGLAIAAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRAAISLGLAEDAPERLDALNATWRSATCRV
LAAATGSRTANAGPVQFDIPLREPLVPDPEPHGAPTPAGRPGGRPWTYTPPVSFDQPLDIDLSPDTVVIAGHGAGTHPNL
AQLPTVAEPTAPAPDNPLHPLALRLLRPKQVIMLGRPTLHRPVSALLADPQVPVYALTTGPRWPDVSGNSQATGTRAVTT
GAPSPAWLHRCEQANRHAVAAVRGQLAAHPLTTGLHVAAAVADALRPGDQLVLGASNPVRDAALVGLDSHHIRVRSNRGV
AGIDGTVSTAIGAALAHEAAHDGRTVALIGDLTFVHDSSGLLIGPTEPTPRRLTIVVSNDNGGGIFELLEQGDPRFSDVS
SRVFGTPHDVDVGALCRAYHVESAQIEVGELAAALDEPGPGMRVLEVKADRSSLRQLHAAIKAAL

Sequences:

>Translated_545_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALADADRAGRIRLHVRIDERTAGYLAIGLAIAAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRAAISLGLAEDAPERLDALNATWRSATCRV
LAAATGSRTANAGPVQFDIPLREPLVPDPEPHGAPTPAGRPGGRPWTYTPPVSFDQPLDIDLSPDTVVIAGHGAGTHPNL
AQLPTVAEPTAPAPDNPLHPLALRLLRPKQVIMLGRPTLHRPVSALLADPQVPVYALTTGPRWPDVSGNSQATGTRAVTT
GAPSPAWLHRCEQANRHAVAAVRGQLAAHPLTTGLHVAAAVADALRPGDQLVLGASNPVRDAALVGLDSHHIRVRSNRGV
AGIDGTVSTAIGAALAHEAAHDGRTVALIGDLTFVHDSSGLLIGPTEPTPRRLTIVVSNDNGGGIFELLEQGDPRFSDVS
SRVFGTPHDVDVGALCRAYHVESAQIEVGELAAALDEPGPGMRVLEVKADRSSLRQLHAAIKAAL
>Mature_545_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALADADRAGRIRLHVRIDERTAGYLAIGLAIAAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRAAISLGLAEDAPERLDALNATWRSATCRV
LAAATGSRTANAGPVQFDIPLREPLVPDPEPHGAPTPAGRPGGRPWTYTPPVSFDQPLDIDLSPDTVVIAGHGAGTHPNL
AQLPTVAEPTAPAPDNPLHPLALRLLRPKQVIMLGRPTLHRPVSALLADPQVPVYALTTGPRWPDVSGNSQATGTRAVTT
GAPSPAWLHRCEQANRHAVAAVRGQLAAHPLTTGLHVAAAVADALRPGDQLVLGASNPVRDAALVGLDSHHIRVRSNRGV
AGIDGTVSTAIGAALAHEAAHDGRTVALIGDLTFVHDSSGLLIGPTEPTPRRLTIVVSNDNGGGIFELLEQGDPRFSDVS
SRVFGTPHDVDVGALCRAYHVESAQIEVGELAAALDEPGPGMRVLEVKADRSSLRQLHAAIKAAL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=510, Percent_Identity=30.1960784313725, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_MYCA1 (A0QLC9)

Other databases:

- EMBL:   CP000479
- RefSeq:   YP_883717.1
- ProteinModelPortal:   A0QLC9
- STRING:   A0QLC9
- EnsemblBacteria:   EBMYCT00000013035
- GeneID:   4529506
- GenomeReviews:   CP000479_GR
- KEGG:   mav:MAV_4588
- TIGR:   MAV_4588
- eggNOG:   COG1165
- GeneTree:   EBGT00050000015760
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- PhylomeDB:   A0QLC9
- ProtClustDB:   PRK07449
- BioCyc:   MAVI243243:MAV_4588-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 56723; Mature: 56723

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALADADRAGRIRLHVRIDERTAGY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAIAAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTM
EEEEHHHHCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCEEEEECCHHHHH
EQLGYFGTQVRAAISLGLAEDAPERLDALNATWRSATCRVLAAATGSRTANAGPVQFDIP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHCCCEEEEEEEECCCCCCCCCCEEEECC
LREPLVPDPEPHGAPTPAGRPGGRPWTYTPPVSFDQPLDIDLSPDTVVIAGHGAGTHPNL
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEECCCCEEEEECCCCCCCCCH
AQLPTVAEPTAPAPDNPLHPLALRLLRPKQVIMLGRPTLHRPVSALLADPQVPVYALTTG
HHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECC
PRWPDVSGNSQATGTRAVTTGAPSPAWLHRCEQANRHAVAAVRGQLAAHPLTTGLHVAAA
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHH
VADALRPGDQLVLGASNPVRDAALVGLDSHHIRVRSNRGVAGIDGTVSTAIGAALAHEAA
HHHHHCCCCEEEEECCCCCCCEEEEECCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHH
HDGRTVALIGDLTFVHDSSGLLIGPTEPTPRRLTIVVSNDNGGGIFELLEQGDPRFSDVS
CCCCEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHH
SRVFGTPHDVDVGALCRAYHVESAQIEVGELAAALDEPGPGMRVLEVKADRSSLRQLHAA
HHHCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHH
IKAAL
HHHCC
>Mature Secondary Structure
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALADADRAGRIRLHVRIDERTAGY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAIAAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTM
EEEEHHHHCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCEEEEECCHHHHH
EQLGYFGTQVRAAISLGLAEDAPERLDALNATWRSATCRVLAAATGSRTANAGPVQFDIP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHCCCEEEEEEEECCCCCCCCCCEEEECC
LREPLVPDPEPHGAPTPAGRPGGRPWTYTPPVSFDQPLDIDLSPDTVVIAGHGAGTHPNL
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEECCCCEEEEECCCCCCCCCH
AQLPTVAEPTAPAPDNPLHPLALRLLRPKQVIMLGRPTLHRPVSALLADPQVPVYALTTG
HHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECC
PRWPDVSGNSQATGTRAVTTGAPSPAWLHRCEQANRHAVAAVRGQLAAHPLTTGLHVAAA
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHH
VADALRPGDQLVLGASNPVRDAALVGLDSHHIRVRSNRGVAGIDGTVSTAIGAALAHEAA
HHHHHCCCCEEEEECCCCCCCEEEEECCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHH
HDGRTVALIGDLTFVHDSSGLLIGPTEPTPRRLTIVVSNDNGGGIFELLEQGDPRFSDVS
CCCCEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHH
SRVFGTPHDVDVGALCRAYHVESAQIEVGELAAALDEPGPGMRVLEVKADRSSLRQLHAA
HHHCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHH
IKAAL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA