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The map label for this gene is galE1

Identifier: 41410091

GI number: 41410091

Start: 4450363

End: 4451490

Strand: Direct

Name: galE1

Synonym: MAP3993

Alternate gene names: 41410091

Gene position: 4450363-4451490 (Clockwise)

Preceding gene: 41410090

Following gene: 41410092

Centisome position: 92.14

GC content: 67.64

Gene sequence:

>1128_bases
GTGGATCCGTCGAATGGGCACAATTCCGGACCGGACGACACCGCCGGCAACACGGTGCATTACCCCAAGATCGTGTTGGT
CACCGGCGCCTGCCGGTTTCTCGGCGGCTACCTGACCGCTCGCCTGGCGCAGAACCCGATGATCAAAGGGGTCATCGCGG
TGGACGCGATCGCCCCGAGCAAGGACATGCTGCGCCGGATGGGTCGCGCCGAGTTCGTCCGCGCCGACATCCGTAATCCC
TTCATAGCCAAGGTGATTCGCAACGGCGACGTCGACACCGTGGTGCACGCGGCGGCCGCGTCGTACGCGCCCCGGTCCGG
CGGCACCGCGGCGCTCAAAGAGATCAACGTGATGGGCGCGATGCAGCTGTTCGCCGCCTGCCAGAAGGCGCCGTCGGTGC
GCCGGGTGGTGCTCAAGTCGACGTCGGAGGTGTACGGCTCCAGTGCGCACGACCCGGTGATGTTCACCGAGGACAGCACC
AGCCGCCGGCCGTTCCGGGCCGGCTTCGCCAAGGACAGCCTGGACATCGAGGCCTATGCGCGCGGTCTGGGGCGGCGCCG
GCCCGACATCGCGGTCACGATCCTGCGGCTGGCCAACATGATCGGCCCGGCGATGGACACCACCCTGTCGCGCTATCTGG
CCGGCCCGTTGGTGCCGACCATTCTCGGCCGGGACGCGCGGCTGCAGCTGCTGCATGAGCAGGACGCGCTGGGCGCGCTG
GAGCGCGCGGCGATGGCCGGCAAGGCGGGCACCTTCAACATCGGCGCCGACGGCATCATCATGCTCTCGCAGGCGATCCG
GCGGGCCGGCCGGATTCCTTTGCCGGTACCGGGTTTCGGCGTCTGGGCGCTGGACTCGTTGCGGCGCGCCAACCGCTACA
ACGAGATCAGTCGCGATCAGTTCGATTATTTGAGCTACGGCCGCGTGATGGACACCAGCCGGATGCGTTCAGAGCTTGGC
TACCAGCCGAAATGGACGACCGCGGAGGCCTTCGACGACTACGTTCGCGGCCGCGGCCTGACTCCCATAATCGATCCAGA
TCGGGTACGCTCCCTGGAGAGTCGCGCCATAGCCCTGGCCCAGCGCTGGGGTAGCCGAAATCCAATTCCGTGGGGTGGGG
TCAGGTAG

Upstream 100 bases:

>100_bases
CAAGCTGCTGCGTCGCACCCGGGTGCAGCGCAGAAAACTCGGCAAGTAACTCCGTCGGCGGCCCGGTGCCGCCGCCCCGC
CCGGCCGTTAGGCTGTTCGG

Downstream 100 bases:

>100_bases
GTATCGTGGCGGGTGAAACCAGAGCGAATGTCATTCCACTGCACACGAATCGGGGTCGGGTAGCGGCGCGTCGGAGAGCC
GATCAACGGGCCGAGTCAGC

Product: GalE1

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDPSNGHNSGPDDTAGNTVHYPKIVLVTGACRFLGGYLTARLAQNPMIKGVIAVDAIAPSKDMLRRMGRAEFVRADIRNP
FIAKVIRNGDVDTVVHAAAASYAPRSGGTAALKEINVMGAMQLFAACQKAPSVRRVVLKSTSEVYGSSAHDPVMFTEDST
SRRPFRAGFAKDSLDIEAYARGLGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTILGRDARLQLLHEQDALGAL
ERAAMAGKAGTFNIGADGIIMLSQAIRRAGRIPLPVPGFGVWALDSLRRANRYNEISRDQFDYLSYGRVMDTSRMRSELG
YQPKWTTAEAFDDYVRGRGLTPIIDPDRVRSLESRAIALAQRWGSRNPIPWGGVR

Sequences:

>Translated_375_residues
MDPSNGHNSGPDDTAGNTVHYPKIVLVTGACRFLGGYLTARLAQNPMIKGVIAVDAIAPSKDMLRRMGRAEFVRADIRNP
FIAKVIRNGDVDTVVHAAAASYAPRSGGTAALKEINVMGAMQLFAACQKAPSVRRVVLKSTSEVYGSSAHDPVMFTEDST
SRRPFRAGFAKDSLDIEAYARGLGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTILGRDARLQLLHEQDALGAL
ERAAMAGKAGTFNIGADGIIMLSQAIRRAGRIPLPVPGFGVWALDSLRRANRYNEISRDQFDYLSYGRVMDTSRMRSELG
YQPKWTTAEAFDDYVRGRGLTPIIDPDRVRSLESRAIALAQRWGSRNPIPWGGVR
>Mature_375_residues
MDPSNGHNSGPDDTAGNTVHYPKIVLVTGACRFLGGYLTARLAQNPMIKGVIAVDAIAPSKDMLRRMGRAEFVRADIRNP
FIAKVIRNGDVDTVVHAAAASYAPRSGGTAALKEINVMGAMQLFAACQKAPSVRRVVLKSTSEVYGSSAHDPVMFTEDST
SRRPFRAGFAKDSLDIEAYARGLGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTILGRDARLQLLHEQDALGAL
ERAAMAGKAGTFNIGADGIIMLSQAIRRAGRIPLPVPGFGVWALDSLRRANRYNEISRDQFDYLSYGRVMDTSRMRSELG
YQPKWTTAEAFDDYVRGRGLTPIIDPDRVRSLESRAIALAQRWGSRNPIPWGGVR

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 40896; Mature: 40896

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSNGHNSGPDDTAGNTVHYPKIVLVTGACRFLGGYLTARLAQNPMIKGVIAVDAIAPS
CCCCCCCCCCCCCCCCCCEECCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
KDMLRRMGRAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASYAPRSGGTAALKEINVMGA
HHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
MQLFAACQKAPSVRRVVLKSTSEVYGSSAHDPVMFTEDSTSRRPFRAGFAKDSLDIEAYA
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHCCCCCCCCHHHHHH
RGLGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTILGRDARLQLLHEQDALGAL
HHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ERAAMAGKAGTFNIGADGIIMLSQAIRRAGRIPLPVPGFGVWALDSLRRANRYNEISRDQ
HHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FDYLSYGRVMDTSRMRSELGYQPKWTTAEAFDDYVRGRGLTPIIDPDRVRSLESRAIALA
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
QRWGSRNPIPWGGVR
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MDPSNGHNSGPDDTAGNTVHYPKIVLVTGACRFLGGYLTARLAQNPMIKGVIAVDAIAPS
CCCCCCCCCCCCCCCCCCEECCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
KDMLRRMGRAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASYAPRSGGTAALKEINVMGA
HHHHHHCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
MQLFAACQKAPSVRRVVLKSTSEVYGSSAHDPVMFTEDSTSRRPFRAGFAKDSLDIEAYA
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHCCCCCCCCHHHHHH
RGLGRRRPDIAVTILRLANMIGPAMDTTLSRYLAGPLVPTILGRDARLQLLHEQDALGAL
HHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ERAAMAGKAGTFNIGADGIIMLSQAIRRAGRIPLPVPGFGVWALDSLRRANRYNEISRDQ
HHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FDYLSYGRVMDTSRMRSELGYQPKWTTAEAFDDYVRGRGLTPIIDPDRVRSLESRAIALA
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
QRWGSRNPIPWGGVR
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]