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The map label for this gene is echA8_2

Identifier: 41410023

GI number: 41410023

Start: 4385187

End: 4386005

Strand: Direct

Name: echA8_2

Synonym: MAP3925

Alternate gene names: 41410023

Gene position: 4385187-4386005 (Clockwise)

Preceding gene: 41410022

Following gene: 41410025

Centisome position: 90.79

GC content: 72.04

Gene sequence:

>819_bases
GTGACCACCGACTTGACCGCCGAAATCGACTCGGGCGTCGCGGTTCTCACGCTCAACCGGCCTGACCGGCTGAACGCCTA
CACCGCCGAGATGGGCGAGCTGCTGGGCCGCGCCTACCGCGAGTGCGACGAGGACGACGACGTCCGGGTCATCGTGCTGA
CCGGCGCCGGGCGCGCCTTTTGCGCCGGGGCCGACTTCTCCGGCGGCGCAAGCCCGTTCGACGCCCCCGCCGGCGACGGC
GCCTTCACCGCCTCACCGATCACCCCGGCGGCGTTCGAGTTGCGCAAACCGGTGATCGCCGCGGTCAACGGCCACGCCGT
CGGGATCGGCCTGACCATCGCGTTGCAGGCCGACATCCGCATCGTCGCCGAGGACGCCAAATACGGTGTGGTGCAAGTGC
GCCGGGGTGTGCTACCCGACTGCATGGCGCACTGGACGCTGCCCCAGTTGACCAACCTGGGGGTGGCCGCCGACCTGCTG
CTCACCGGGCGGATGTTCGGCGGCGCCGAGGCGACCGCGCTCGGCATCGCCAACCGCGCGCTGCCCGCCGATCGGGTGCT
CGACGAGGCGATGGCGCTGGCCCGCGACATCGCCGTCAACGTGGCGCCGATGTCGGCGGCCCTGTGCAAACGCCTGCTGT
GGGACAGCGCGATCAATCGCTACCCACCGCGACAGGTCGCCGCGCTGGAAACCCTGCTGCACGAACGGGTGATGGGAGGC
GGCGACGCCGCCGAGGGCGTCGCCGCGTTCCTCGAGCACCGGGCGCCGCGCTTCACCGCGCGGCTGTCGTCGGAGTGGGA
GCCGCTGCCGGAGCCATGA

Upstream 100 bases:

>100_bases
AGCACGCCGACGAGGTGTGCTGTTACTTTCCGGGCTACACCCCCCGCCCCGCGGACGTCGCCGACCTGATCGGCGCCCTG
CACCGGGTGCCGGCGCTGCC

Downstream 100 bases:

>100_bases
CGGCGGCGCGCCGCATCAGCCGCGGTGCCACGATCCGGTGCAGCGGGCCGACCAGCGCCCAGACCGCTCGCGCGGCGAGC
TTGTGACGGTAATGCAGGCG

Product: EchA8_2

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTTDLTAEIDSGVAVLTLNRPDRLNAYTAEMGELLGRAYRECDEDDDVRVIVLTGAGRAFCAGADFSGGASPFDAPAGDG
AFTASPITPAAFELRKPVIAAVNGHAVGIGLTIALQADIRIVAEDAKYGVVQVRRGVLPDCMAHWTLPQLTNLGVAADLL
LTGRMFGGAEATALGIANRALPADRVLDEAMALARDIAVNVAPMSAALCKRLLWDSAINRYPPRQVAALETLLHERVMGG
GDAAEGVAAFLEHRAPRFTARLSSEWEPLPEP

Sequences:

>Translated_272_residues
MTTDLTAEIDSGVAVLTLNRPDRLNAYTAEMGELLGRAYRECDEDDDVRVIVLTGAGRAFCAGADFSGGASPFDAPAGDG
AFTASPITPAAFELRKPVIAAVNGHAVGIGLTIALQADIRIVAEDAKYGVVQVRRGVLPDCMAHWTLPQLTNLGVAADLL
LTGRMFGGAEATALGIANRALPADRVLDEAMALARDIAVNVAPMSAALCKRLLWDSAINRYPPRQVAALETLLHERVMGG
GDAAEGVAAFLEHRAPRFTARLSSEWEPLPEP
>Mature_271_residues
TTDLTAEIDSGVAVLTLNRPDRLNAYTAEMGELLGRAYRECDEDDDVRVIVLTGAGRAFCAGADFSGGASPFDAPAGDGA
FTASPITPAAFELRKPVIAAVNGHAVGIGLTIALQADIRIVAEDAKYGVVQVRRGVLPDCMAHWTLPQLTNLGVAADLLL
TGRMFGGAEATALGIANRALPADRVLDEAMALARDIAVNVAPMSAALCKRLLWDSAINRYPPRQVAALETLLHERVMGGG
DAAEGVAAFLEHRAPRFTARLSSEWEPLPEP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=29.4820717131474, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=28.515625, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI68989263, Length=237, Percent_Identity=29.957805907173, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI150378541, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=185, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI45643119, Length=185, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI260275230, Length=185, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI25453481, Length=217, Percent_Identity=21.6589861751152, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI51538573, Length=217, Percent_Identity=21.6589861751152, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=172, Percent_Identity=24.4186046511628, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI51538581, Length=172, Percent_Identity=24.4186046511628, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=271, Percent_Identity=34.6863468634686, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI221142681, Length=280, Percent_Identity=31.4285714285714, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787659, Length=268, Percent_Identity=29.1044776119403, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788682, Length=206, Percent_Identity=27.6699029126214, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=25.1282051282051, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=24.3346007604563, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=32.295719844358, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=33.1578947368421, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=199, Percent_Identity=29.6482412060301, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=29.126213592233, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=30.316742081448, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=31.6939890710383, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=32.9457364341085, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357171, Length=193, Percent_Identity=30.5699481865285, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=28.8, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24654903, Length=188, Percent_Identity=27.1276595744681, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28685; Mature: 28554

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDLTAEIDSGVAVLTLNRPDRLNAYTAEMGELLGRAYRECDEDDDVRVIVLTGAGRAF
CCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
CAGADFSGGASPFDAPAGDGAFTASPITPAAFELRKPVIAAVNGHAVGIGLTIALQADIR
EECCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHCCCEEEEECCCEEEEEEEEEEEECEE
IVAEDAKYGVVQVRRGVLPDCMAHWTLPQLTNLGVAADLLLTGRMFGGAEATALGIANRA
EEECCCCCCHHHHHHCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHC
LPADRVLDEAMALARDIAVNVAPMSAALCKRLLWDSAINRYPPRQVAALETLLHERVMGG
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GDAAEGVAAFLEHRAPRFTARLSSEWEPLPEP
CCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTDLTAEIDSGVAVLTLNRPDRLNAYTAEMGELLGRAYRECDEDDDVRVIVLTGAGRAF
CCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
CAGADFSGGASPFDAPAGDGAFTASPITPAAFELRKPVIAAVNGHAVGIGLTIALQADIR
EECCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHCCCEEEEECCCEEEEEEEEEEEECEE
IVAEDAKYGVVQVRRGVLPDCMAHWTLPQLTNLGVAADLLLTGRMFGGAEATALGIANRA
EEECCCCCCHHHHHHCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHC
LPADRVLDEAMALARDIAVNVAPMSAALCKRLLWDSAINRYPPRQVAALETLLHERVMGG
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GDAAEGVAAFLEHRAPRFTARLSSEWEPLPEP
CCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA