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The map label for this gene is thiO/thiG [H]

Identifier: 41409996

GI number: 41409996

Start: 4362984

End: 4364063

Strand: Direct

Name: thiO/thiG [H]

Synonym: MAP3898

Alternate gene names: 41409996

Gene position: 4362984-4364063 (Clockwise)

Preceding gene: 41409994

Following gene: 41409997

Centisome position: 90.34

GC content: 74.72

Gene sequence:

>1080_bases
GTGGGCGCGCCCGCCCTTACCGTCACACCTGATCCGGATCATGCCGGCGAAGGGAGGCCGCGAATGCCCTCGGACTGCGG
GTCGCTGGCCGTCGTCGGTGGCGGGGTCATCGGGCTGGCGGTGGCGCGCCGGGCCGCGCAGGCCGGGTGGTCGGTGCGGG
TGCACCGCAGCGGCGAGCGGGGCGCCTCCTGGGTGGCCGGCGGCATGCTGGCCCCGCACAGCGAAGGCTGGCCCGGCGAG
GAGCGACTGTTGCGGCTGGGCCTGGAATCGCTGCGGCTGTGGCGGGAGGGCGGTTTCCTCGACGGGCTGCCGGGGCGGCT
GGTCACCGCGCGCGAGTCCCTGGTGGTGGCGGTCGACCGCGCCGACGTCGCCGACCTGCGCACCGTCGCGGATTGGCTGT
CCGCACAAGGACATCCGGTGGTCTGGGAGTCGGCGGCCCGCGACGTCGAACCCCTGCTGGCGCAGGGCATCCGGCACGGC
TTCACTGCGCCCACCGAACTGGCCGTGGACAACCGCGCGGTGCTCGAGGCGCTGGACGCGGCCTGCGAGCGGCTCGGGGT
GCGCTGGGCGCCGCCGCTGCACGACCTGGCGGAGGCCGACGCCGACGCGGTGGTGATCGCCAACGGCATCGACGCGCAGG
CGTTGTGGCCGGGCCTGCCGGTGCGCCCGGTGAAGGGCGAAGTGCTGCGGCTGCGGTGGCGAAAGGGTTGTATGCCGGTG
CCGCAGCGGGTCATTCGGGCCCGCATGCACGGCCGGCAGGTGTACCTGGTGCCACGCGCGGACGGGGTGGTGGTCGGCGC
CACCCAGTACGAACACGGGCGAGACACCGCCCCGGTGGTCTCCGGGGTGCGGGACCTGCTCGACGACGCCTGCGCGGTGC
TGCCGGCGCTGGGCGAATACGAGCTGGCCGAGTGCGGCGCCGGGCTGCGCCCGATGACGCCCGACAATCTGCCGCTGGTG
GGCCGCCTGGACGCGCGGACCCTGGTTGCCGCCGGTCACGGCCGGTCGGGATTCCTGTTGGCGCCCTGGACGGCAGAACA
GATCGTGTCCGAACTGGCCCCGGTGGGAGCACACCGATGA

Upstream 100 bases:

>100_bases
AGAGTTGCCAGACGTTGATGCACACCGCGACGCTAGCGCGCTAGCGTGGAACCCGAACAACTCGTTTTAGACACGGGAGT
CCCGGGACCGGGGTCTGAGA

Downstream 100 bases:

>100_bases
TCCCGCCGGCGACGATGCTTCAGCGAAGCGATGAGGAGGAGCGGCGCCAATGATCGTCGTAGTCAACGAGCAAAAGGTCG
ACGTCGACGCGCACACCACG

Product: hypothetical protein

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGAPALTVTPDPDHAGEGRPRMPSDCGSLAVVGGGVIGLAVARRAAQAGWSVRVHRSGERGASWVAGGMLAPHSEGWPGE
ERLLRLGLESLRLWREGGFLDGLPGRLVTARESLVVAVDRADVADLRTVADWLSAQGHPVVWESAARDVEPLLAQGIRHG
FTAPTELAVDNRAVLEALDAACERLGVRWAPPLHDLAEADADAVVIANGIDAQALWPGLPVRPVKGEVLRLRWRKGCMPV
PQRVIRARMHGRQVYLVPRADGVVVGATQYEHGRDTAPVVSGVRDLLDDACAVLPALGEYELAECGAGLRPMTPDNLPLV
GRLDARTLVAAGHGRSGFLLAPWTAEQIVSELAPVGAHR

Sequences:

>Translated_359_residues
MGAPALTVTPDPDHAGEGRPRMPSDCGSLAVVGGGVIGLAVARRAAQAGWSVRVHRSGERGASWVAGGMLAPHSEGWPGE
ERLLRLGLESLRLWREGGFLDGLPGRLVTARESLVVAVDRADVADLRTVADWLSAQGHPVVWESAARDVEPLLAQGIRHG
FTAPTELAVDNRAVLEALDAACERLGVRWAPPLHDLAEADADAVVIANGIDAQALWPGLPVRPVKGEVLRLRWRKGCMPV
PQRVIRARMHGRQVYLVPRADGVVVGATQYEHGRDTAPVVSGVRDLLDDACAVLPALGEYELAECGAGLRPMTPDNLPLV
GRLDARTLVAAGHGRSGFLLAPWTAEQIVSELAPVGAHR
>Mature_358_residues
GAPALTVTPDPDHAGEGRPRMPSDCGSLAVVGGGVIGLAVARRAAQAGWSVRVHRSGERGASWVAGGMLAPHSEGWPGEE
RLLRLGLESLRLWREGGFLDGLPGRLVTARESLVVAVDRADVADLRTVADWLSAQGHPVVWESAARDVEPLLAQGIRHGF
TAPTELAVDNRAVLEALDAACERLGVRWAPPLHDLAEADADAVVIANGIDAQALWPGLPVRPVKGEVLRLRWRKGCMPVP
QRVIRARMHGRQVYLVPRADGVVVGATQYEHGRDTAPVVSGVRDLLDDACAVLPALGEYELAECGAGLRPMTPDNLPLVG
RLDARTLVAAGHGRSGFLLAPWTAEQIVSELAPVGAHR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 38106; Mature: 37975

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAPALTVTPDPDHAGEGRPRMPSDCGSLAVVGGGVIGLAVARRAAQAGWSVRVHRSGER
CCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEECCCCC
GASWVAGGMLAPHSEGWPGEERLLRLGLESLRLWREGGFLDGLPGRLVTARESLVVAVDR
CCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECC
ADVADLRTVADWLSAQGHPVVWESAARDVEPLLAQGIRHGFTAPTELAVDNRAVLEALDA
CCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHH
ACERLGVRWAPPLHDLAEADADAVVIANGIDAQALWPGLPVRPVKGEVLRLRWRKGCMPV
HHHHCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHCCCCCCCCCCCHHEEEHHHCCCCCH
PQRVIRARMHGRQVYLVPRADGVVVGATQYEHGRDTAPVVSGVRDLLDDACAVLPALGEY
HHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
ELAECGAGLRPMTPDNLPLVGRLDARTLVAAGHGRSGFLLAPWTAEQIVSELAPVGAHR
HHHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GAPALTVTPDPDHAGEGRPRMPSDCGSLAVVGGGVIGLAVARRAAQAGWSVRVHRSGER
CCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEECCCCC
GASWVAGGMLAPHSEGWPGEERLLRLGLESLRLWREGGFLDGLPGRLVTARESLVVAVDR
CCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECC
ADVADLRTVADWLSAQGHPVVWESAARDVEPLLAQGIRHGFTAPTELAVDNRAVLEALDA
CCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHH
ACERLGVRWAPPLHDLAEADADAVVIANGIDAQALWPGLPVRPVKGEVLRLRWRKGCMPV
HHHHCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHCCCCCCCCCCCHHEEEHHHCCCCCH
PQRVIRARMHGRQVYLVPRADGVVVGATQYEHGRDTAPVVSGVRDLLDDACAVLPALGEY
HHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
ELAECGAGLRPMTPDNLPLVGRLDARTLVAAGHGRSGFLLAPWTAEQIVSELAPVGAHR
HHHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]