Click here to switch to the map view.

The map label for this gene is otsB2

Identifier: 41409576

GI number: 41409576

Start: 3865245

End: 3866420

Strand: Direct

Name: otsB2

Synonym: MAP3478

Alternate gene names: 41409576

Gene position: 3865245-3866420 (Clockwise)

Preceding gene: 41409575

Following gene: 41409578

Centisome position: 80.03

GC content: 75.51

Gene sequence:

>1176_bases
ATGGGCGAATCGGGGCCGGTGGTGATCGACCCGCGCCGCCACGACGCGGTGCTGTTCGGGGTCGGCGACGCGCTGGGCTC
GGCACTGGCGAGCCAACTGGGACAGATCGGGGTGGGCACCGCGGCGTTCGCGGCGGACGGCCCGGCGGCGGCGGCCGACC
GGCTGCGGGTCCGCCCGGGCCGCTGCGTGGTGGTGGCCGGCGACCCCGCGGCCGTCGAGGCGGCCCGCACCGCCGGGTTC
GCGTTGGTGATCGGGCTGGCGCCCGTCGGGCGCGACGGCGACGGGCTGCGCGGGGCCGGGGCCGACGCGGTGGTCGCCGA
GCTGGAACAGATCACCGTGCGCACCGGCGACCGCCGGATGTCGCAGCTGCCGGATGCCTCCCAGGCCCTGACCGGCGGCG
CCGACGGGCTGGCCGGTCGGCACCCGGCGGTGTTCTTCGACTTCGACGGCACGCTGTCGGACATCGTCGACGATCCCGAC
GCGGCCCGCCCGGTGGCCGGGGCCACCGCGGCGCTGACCCGGCTGGCCGCGCGTTGCCCGGTCGCGGTGCTGTCCGGGCG
CGATCTTGCTGACGTGACGAAACGCGTTGGGGTGCTGGGGATCTGGTATGCGGGAAGCCACGGTTTCGAGCTGACCGCAC
CCGACGGCAGCCACCACCAGAACGATGACGCCGCCGCGGCCATCCCGGTGCTGGCGCAAGCGGCCGGGCGGCTGCGCGAT
GAGCTCGGCGCGATACCGGGTGTTGTGGTGGAGCACAAGCGATTCGGCGTCGCGGTGCACTACCGCAACGCGGCACGCGA
CCGCGTCGGCGAGGTGGCGGCGGCGGTGCGCGCCGCGGGCCGGCACGACGCGCTGCGGGTGACCACCGGCCGCGAGGTCA
TCGAGCTGCGCCCGGACCTGGACTGGGACAAGGGCAAGACGCTGCACTGGGTGATCGAGCACCTGCGCCGGTCCGGGTCG
GGTGCGCTGACGCCGGTCTACCTCGGCGACGACATCACCGACGAGGACGCGTTCGACGCGGTGCGCGGCGGGCCCGTCCA
GGGCGTGCCAATCCTGGTGCGGCACAACGACGACGGCGACCGCGCCACCGCGGCCCTGTTCGCGCTGGACAGTCCCGCGC
GGGCCGCCGAGTTCACCGAGCGGCTGGCCGATCAGCTCGAACGCGGCGAGGGTTAG

Upstream 100 bases:

>100_bases
CCGCTGGGCCGGGTGCCGCCCGCGCACCCGTCGGCGCCCGGGCACGCGCGTCACTGACCGGCTCGGGGCGCGACCGTGCC
GCCGTGAGAAGGTTTTCCTC

Downstream 100 bases:

>100_bases
GCGTACTCCACCGCGCCGTCGTCGAGCACCACCCGGGTGTTGGAGCCGTCCGGGTTCGACGCCTCGGTGCGGAACACCGC
CTTGCCCGGCTCGGTGCGCC

Product: OtsB2

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MGESGPVVIDPRRHDAVLFGVGDALGSALASQLGQIGVGTAAFAADGPAAAADRLRVRPGRCVVVAGDPAAVEAARTAGF
ALVIGLAPVGRDGDGLRGAGADAVVAELEQITVRTGDRRMSQLPDASQALTGGADGLAGRHPAVFFDFDGTLSDIVDDPD
AARPVAGATAALTRLAARCPVAVLSGRDLADVTKRVGVLGIWYAGSHGFELTAPDGSHHQNDDAAAAIPVLAQAAGRLRD
ELGAIPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRAAGRHDALRVTTGREVIELRPDLDWDKGKTLHWVIEHLRRSGS
GALTPVYLGDDITDEDAFDAVRGGPVQGVPILVRHNDDGDRATAALFALDSPARAAEFTERLADQLERGEG

Sequences:

>Translated_391_residues
MGESGPVVIDPRRHDAVLFGVGDALGSALASQLGQIGVGTAAFAADGPAAAADRLRVRPGRCVVVAGDPAAVEAARTAGF
ALVIGLAPVGRDGDGLRGAGADAVVAELEQITVRTGDRRMSQLPDASQALTGGADGLAGRHPAVFFDFDGTLSDIVDDPD
AARPVAGATAALTRLAARCPVAVLSGRDLADVTKRVGVLGIWYAGSHGFELTAPDGSHHQNDDAAAAIPVLAQAAGRLRD
ELGAIPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRAAGRHDALRVTTGREVIELRPDLDWDKGKTLHWVIEHLRRSGS
GALTPVYLGDDITDEDAFDAVRGGPVQGVPILVRHNDDGDRATAALFALDSPARAAEFTERLADQLERGEG
>Mature_390_residues
GESGPVVIDPRRHDAVLFGVGDALGSALASQLGQIGVGTAAFAADGPAAAADRLRVRPGRCVVVAGDPAAVEAARTAGFA
LVIGLAPVGRDGDGLRGAGADAVVAELEQITVRTGDRRMSQLPDASQALTGGADGLAGRHPAVFFDFDGTLSDIVDDPDA
ARPVAGATAALTRLAARCPVAVLSGRDLADVTKRVGVLGIWYAGSHGFELTAPDGSHHQNDDAAAAIPVLAQAAGRLRDE
LGAIPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRAAGRHDALRVTTGREVIELRPDLDWDKGKTLHWVIEHLRRSGSG
ALTPVYLGDDITDEDAFDAVRGGPVQGVPILVRHNDDGDRATAALFALDSPARAAEFTERLADQLERGEG

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=205, Percent_Identity=29.7560975609756, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221473368, Length=231, Percent_Identity=32.9004329004329, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20129307, Length=231, Percent_Identity=32.9004329004329, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24582490, Length=231, Percent_Identity=32.9004329004329, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19920676, Length=214, Percent_Identity=31.3084112149533, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI20129309, Length=204, Percent_Identity=25, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 40427; Mature: 40295

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGESGPVVIDPRRHDAVLFGVGDALGSALASQLGQIGVGTAAFAADGPAAAADRLRVRPG
CCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEECCC
RCVVVAGDPAAVEAARTAGFALVIGLAPVGRDGDGLRGAGADAVVAELEQITVRTGDRRM
CEEEEECCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
SQLPDASQALTGGADGLAGRHPAVFFDFDGTLSDIVDDPDAARPVAGATAALTRLAARCP
HHCCCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
VAVLSGRDLADVTKRVGVLGIWYAGSHGFELTAPDGSHHQNDDAAAAIPVLAQAAGRLRD
EEEECCCCHHHHHHHCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
ELGAIPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRAAGRHDALRVTTGREVIELRPDL
HHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCC
DWDKGKTLHWVIEHLRRSGSGALTPVYLGDDITDEDAFDAVRGGPVQGVPILVRHNDDGD
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCEEEEEECCCCC
RATAALFALDSPARAAEFTERLADQLERGEG
HHEEEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GESGPVVIDPRRHDAVLFGVGDALGSALASQLGQIGVGTAAFAADGPAAAADRLRVRPG
CCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEECCC
RCVVVAGDPAAVEAARTAGFALVIGLAPVGRDGDGLRGAGADAVVAELEQITVRTGDRRM
CEEEEECCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
SQLPDASQALTGGADGLAGRHPAVFFDFDGTLSDIVDDPDAARPVAGATAALTRLAARCP
HHCCCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
VAVLSGRDLADVTKRVGVLGIWYAGSHGFELTAPDGSHHQNDDAAAAIPVLAQAAGRLRD
EEEECCCCHHHHHHHCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
ELGAIPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRAAGRHDALRVTTGREVIELRPDL
HHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCC
DWDKGKTLHWVIEHLRRSGSGALTPVYLGDDITDEDAFDAVRGGPVQGVPILVRHNDDGD
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCEEEEEECCCCC
RATAALFALDSPARAAEFTERLADQLERGEG
HHEEEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA