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The map label for this gene is nuoI_2

Identifier: 41409307

GI number: 41409307

Start: 3560086

End: 3560631

Strand: Direct

Name: nuoI_2

Synonym: MAP3209

Alternate gene names: 41409307

Gene position: 3560086-3560631 (Clockwise)

Preceding gene: 41409306

Following gene: 41409308

Centisome position: 73.71

GC content: 68.68

Gene sequence:

>546_bases
GTGGCTAAATTCTTGGACGCGGTCGCCGGATTCGGCGTGACGTTCGCGTCGATGTTCAAAAAGCATGTCACCGAGGAGTA
TCCGGAGAAGCCGGGCCCGGTCGCGCCGCGCTACCACGGCCGTCACCAGCTCAACCGTTACCCCGACGGGCTGGAGAAGT
GCATCGGCTGCGAGCTGTGCGCGTGGGCCTGCCCGGCCGACGCGATCTACGTCGAGGGCGCCGACAACACCGAGGAACTG
CGGTATTCGCCCGGGGAACGCTACGGCCGGGTGTACCAGATCAACTACCTGCGCTGCATCGGCTGCGGGCTGTGCGTCGA
GGCCTGCCCGACCCGGGCGCTGACGATGACCAACGACTACGAGATGGCCGACGACAACCGCGCCGACCTGATCTACGAGA
AGGACCGGCTGCTGGCCCCGCTGCTGCCCGAGATGACCGCGCCGCCGCATCCCCGCGCCCCCGGCGCCACCGATAAGGAC
TACTACCTGGGCAATGTGACCGCGCACGGGCTGCGGGAGGCCCAGCGGGCCGGGGAGCCGCGGTGA

Upstream 100 bases:

>100_bases
AAGGCCAAGCAGCTGCCCACCGCGCCGGATTCCACCCCGGTATTCCCGACACCGCCGCTGCCGGCGAAAGCGCTGGCGGC
CGCGAGTAAGGAGAAAGCAC

Downstream 100 bases:

>100_bases
GTGCCTTCGCCGGCCACGCCGTCGCCGCCACGCTGGCCTCCAGCTATTCCCAGGACACCATCGTCCGCACATCCACCGGC
GAGGCAGTGACGTTCTGGGT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 2; NDH-1 subunit I 2

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MAKFLDAVAGFGVTFASMFKKHVTEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGADNTEEL
RYSPGERYGRVYQINYLRCIGCGLCVEACPTRALTMTNDYEMADDNRADLIYEKDRLLAPLLPEMTAPPHPRAPGATDKD
YYLGNVTAHGLREAQRAGEPR

Sequences:

>Translated_181_residues
MAKFLDAVAGFGVTFASMFKKHVTEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGADNTEEL
RYSPGERYGRVYQINYLRCIGCGLCVEACPTRALTMTNDYEMADDNRADLIYEKDRLLAPLLPEMTAPPHPRAPGATDKD
YYLGNVTAHGLREAQRAGEPR
>Mature_180_residues
AKFLDAVAGFGVTFASMFKKHVTEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGADNTEELR
YSPGERYGRVYQINYLRCIGCGLCVEACPTRALTMTNDYEMADDNRADLIYEKDRLLAPLLPEMTAPPHPRAPGATDKDY
YLGNVTAHGLREAQRAGEPR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=135, Percent_Identity=37.037037037037, Blast_Score=107, Evalue=5e-24,
Organism=Escherichia coli, GI1788617, Length=140, Percent_Identity=43.5714285714286, Blast_Score=116, Evalue=8e-28,
Organism=Escherichia coli, GI1789075, Length=110, Percent_Identity=32.7272727272727, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=135, Percent_Identity=38.5185185185185, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17864306, Length=135, Percent_Identity=40, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI2_MYCPA (Q73V07)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_962143.1
- ProteinModelPortal:   Q73V07
- SMR:   Q73V07
- EnsemblBacteria:   EBMYCT00000038717
- GeneID:   2718717
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP3209
- NMPDR:   fig|262316.1.peg.3209
- GeneTree:   EBGT00050000017055
- HOGENOM:   HBG724359
- OMA:   VEGGDNT
- ProtClustDB:   PRK05888
- BRENDA:   1.6.99.5
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20219; Mature: 20088

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFLDAVAGFGVTFASMFKKHVTEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELC
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
AWACPADAIYVEGADNTEELRYSPGERYGRVYQINYLRCIGCGLCVEACPTRALTMTNDY
HHCCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEECCCC
EMADDNRADLIYEKDRLLAPLLPEMTAPPHPRAPGATDKDYYLGNVTAHGLREAQRAGEP
CCCCCCCCCEEECCCHHHHHHCHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCC
R
C
>Mature Secondary Structure 
AKFLDAVAGFGVTFASMFKKHVTEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
AWACPADAIYVEGADNTEELRYSPGERYGRVYQINYLRCIGCGLCVEACPTRALTMTNDY
HHCCCCCEEEEECCCCHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEECCCC
EMADDNRADLIYEKDRLLAPLLPEMTAPPHPRAPGATDKDYYLGNVTAHGLREAQRAGEP
CCCCCCCCCEEECCCHHHHHHCHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA