Click here to switch to the map view.

The map label for this gene is ligB

Identifier: 41409215

GI number: 41409215

Start: 3465504

End: 3467063

Strand: Direct

Name: ligB

Synonym: MAP3117

Alternate gene names: 41409215

Gene position: 3465504-3467063 (Clockwise)

Preceding gene: 41409212

Following gene: 41409216

Centisome position: 71.75

GC content: 74.1

Gene sequence:

>1560_bases
TTGTCACCGAGCCATGCCAAGCTGGCGACCGTACTGCTTTTCGACGTGGCGACGGCGTCGGCCGACGTCGGGGGCACGCC
GTCGCGGCTGACCAAAGTCGCCCGCATCGCCGACCTGCTGCGCCGGGCCGCACCCAACGCCGCCCTGGTCGCGATCGTCG
TGTCGTGGCTGTCCGGTGAGTTGCGGCAGCGCCAGATCGGGGTCGGCTGGGCGGCGTTGCGCTCGCGCCCGCCCGCGGCC
GCACATCCCACGCTGACCGTCGTCGCGGTGGACGCCGCCTTCGCCGAGATCGGCGCCGTGGCGGGCAAGGGCGCCCAGGC
GCGCCGCGCGGCCCTGCTGAACGCGTTGTTCGCCGCGGCCACCGAGACCGAGCAGACGTTTCTGCTGCGGCTGCTGGGCG
GCGAACTGCGCCAGGGCGCGCTGGCCGGGATCATGGCCGACGCCGTCGCCAGGGCCGCCGGGATACCGGCCGCGGCGGTG
CAGCGCGCCGCGATGCTGGGCGGTGACCTGCCGGCGGTGGCGGCGGCCGCGCTGTCCGGGGAGGCGTCCGCGCTGGACGC
CTTCACGCTGCGGGTGGGCCGGCCGGTCGCGCCGATGCTGGCCCAGACCGCGGCGGGTGTGGCCCAGGCGATCGAACGCC
ACGGCGGGCAGGCGATTTTCGAGGCCAAGCTGGACGGGGCGCGGGTGCAGATCCACCGCGCCGGCGACCAGGTCACCGTC
TACACCCGAAGCCTCGACGACGTCACCGCCCGACTGCCCGAGGTGGTGACCGCGACGCTGGCGCTGCCGGTCGAGGCCCT
GATCGCCGACGGTGAGGCGATCGCGCTGCGGCCGGACAACTCACCGCAGCGCTTCCAGGTCACCGCCTCACGCTTCGGCC
GCTCGCTCGATGTCGCAGCAGCCGTTGCGGCGCAACCACTTTCGGTGTTCATCTTCGACATCCTGCACTGCGACGGCATC
GACCTGCTGGACGCGCCGACCACCGACCGGCTGGCCGCGCTGGACGCCCTGGTGCCGCCCGCGCAGCGGGTCGACCGGCT
GCTCACCGCCGACCCCGACGCGGCGGGCCGGTTCCTGGAGGCGACGCTGGCCGCCGGGCACGAGGGCGTGATGGCCAAGG
CGCCGGGCGCGCCGTATCAGGCGGGCCGGCGCGGGGCGGGCTGGCTCAAGGTCAAGCCGGTGCACACGCTGGATCTAGTG
GTGCTCGCCGTGGAGTGGGGCTCGGGGCGGCGCCGCGGCAAGCTGTCCAACATCCACCTGGGCGCCCGCGACCCGGCCAC
CGGCGAATTCGTCATGGTGGGAAAGACTTTCAAGGGCATGACCGACGCGATGCTGGACTGGCAGACGGCCCGGTTCACCG
AGCTGGCCGTCGGCGGCACGGACGGCTACGTGGTGCGGGTGCGACCCGAGCAGGTGGTCGAGGTGGCGGTGGACGGTGTG
CAGAAATCCTCGCGCTACCCCGGCGGGCTGGCGCTGCGGTTCGCCCGGGTGCTGCGCTACCGCGACGACAAGGGCCCCGC
GGAGGCCGACACCATCGACGCCGTCCGGGCGCTGTACTGA

Upstream 100 bases:

>100_bases
TTCCCGTTGATCTCCATCCGGACCTCCCATTGTCAGGCTTGTCAGAATCCATGCGATTTGTAACGTTACGTAGGCACGCT
AGCGTGTCCGCTCCAGGCGC

Downstream 100 bases:

>100_bases
TCACCCGCTCGACGCGCGCCGGTGCTCTTGCGCGCGTAGGGTTTCCGCCGTGGCAATCACCGAGCGCGACGACGGCGTCA
GCTACGTGCACACCGACCAC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSPSHAKLATVLLFDVATASADVGGTPSRLTKVARIADLLRRAAPNAALVAIVVSWLSGELRQRQIGVGWAALRSRPPAA
AHPTLTVVAVDAAFAEIGAVAGKGAQARRAALLNALFAAATETEQTFLLRLLGGELRQGALAGIMADAVARAAGIPAAAV
QRAAMLGGDLPAVAAAALSGEASALDAFTLRVGRPVAPMLAQTAAGVAQAIERHGGQAIFEAKLDGARVQIHRAGDQVTV
YTRSLDDVTARLPEVVTATLALPVEALIADGEAIALRPDNSPQRFQVTASRFGRSLDVAAAVAAQPLSVFIFDILHCDGI
DLLDAPTTDRLAALDALVPPAQRVDRLLTADPDAAGRFLEATLAAGHEGVMAKAPGAPYQAGRRGAGWLKVKPVHTLDLV
VLAVEWGSGRRRGKLSNIHLGARDPATGEFVMVGKTFKGMTDAMLDWQTARFTELAVGGTDGYVVRVRPEQVVEVAVDGV
QKSSRYPGGLALRFARVLRYRDDKGPAEADTIDAVRALY

Sequences:

>Translated_519_residues
MSPSHAKLATVLLFDVATASADVGGTPSRLTKVARIADLLRRAAPNAALVAIVVSWLSGELRQRQIGVGWAALRSRPPAA
AHPTLTVVAVDAAFAEIGAVAGKGAQARRAALLNALFAAATETEQTFLLRLLGGELRQGALAGIMADAVARAAGIPAAAV
QRAAMLGGDLPAVAAAALSGEASALDAFTLRVGRPVAPMLAQTAAGVAQAIERHGGQAIFEAKLDGARVQIHRAGDQVTV
YTRSLDDVTARLPEVVTATLALPVEALIADGEAIALRPDNSPQRFQVTASRFGRSLDVAAAVAAQPLSVFIFDILHCDGI
DLLDAPTTDRLAALDALVPPAQRVDRLLTADPDAAGRFLEATLAAGHEGVMAKAPGAPYQAGRRGAGWLKVKPVHTLDLV
VLAVEWGSGRRRGKLSNIHLGARDPATGEFVMVGKTFKGMTDAMLDWQTARFTELAVGGTDGYVVRVRPEQVVEVAVDGV
QKSSRYPGGLALRFARVLRYRDDKGPAEADTIDAVRALY
>Mature_518_residues
SPSHAKLATVLLFDVATASADVGGTPSRLTKVARIADLLRRAAPNAALVAIVVSWLSGELRQRQIGVGWAALRSRPPAAA
HPTLTVVAVDAAFAEIGAVAGKGAQARRAALLNALFAAATETEQTFLLRLLGGELRQGALAGIMADAVARAAGIPAAAVQ
RAAMLGGDLPAVAAAALSGEASALDAFTLRVGRPVAPMLAQTAAGVAQAIERHGGQAIFEAKLDGARVQIHRAGDQVTVY
TRSLDDVTARLPEVVTATLALPVEALIADGEAIALRPDNSPQRFQVTASRFGRSLDVAAAVAAQPLSVFIFDILHCDGID
LLDAPTTDRLAALDALVPPAQRVDRLLTADPDAAGRFLEATLAAGHEGVMAKAPGAPYQAGRRGAGWLKVKPVHTLDLVV
LAVEWGSGRRRGKLSNIHLGARDPATGEFVMVGKTFKGMTDAMLDWQTARFTELAVGGTDGYVVRVRPEQVVEVAVDGVQ
KSSRYPGGLALRFARVLRYRDDKGPAEADTIDAVRALY

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=503, Percent_Identity=27.4353876739563, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI46255052, Length=490, Percent_Identity=24.2857142857143, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI23199993, Length=490, Percent_Identity=24.2857142857143, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI148539894, Length=490, Percent_Identity=24.2857142857143, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71985269, Length=505, Percent_Identity=27.3267326732673, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320038, Length=520, Percent_Identity=27.3076923076923, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324578, Length=259, Percent_Identity=25.0965250965251, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24762424, Length=499, Percent_Identity=27.0541082164329, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI85815841, Length=458, Percent_Identity=26.4192139737991, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 54126; Mature: 53995

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSHAKLATVLLFDVATASADVGGTPSRLTKVARIADLLRRAAPNAALVAIVVSWLSGE
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCH
LRQRQIGVGWAALRSRPPAAAHPTLTVVAVDAAFAEIGAVAGKGAQARRAALLNALFAAA
HHHHHCCCCHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TETEQTFLLRLLGGELRQGALAGIMADAVARAAGIPAAAVQRAAMLGGDLPAVAAAALSG
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCC
EASALDAFTLRVGRPVAPMLAQTAAGVAQAIERHGGQAIFEAKLDGARVQIHRAGDQVTV
CCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEE
YTRSLDDVTARLPEVVTATLALPVEALIADGEAIALRPDNSPQRFQVTASRFGRSLDVAA
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCHHHHH
AVAAQPLSVFIFDILHCDGIDLLDAPTTDRLAALDALVPPAQRVDRLLTADPDAAGRFLE
HHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHH
ATLAAGHEGVMAKAPGAPYQAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHL
HHHHCCCCCCEECCCCCCHHCCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEE
GARDPATGEFVMVGKTFKGMTDAMLDWQTARFTELAVGGTDGYVVRVRPEQVVEVAVDGV
CCCCCCCCCEEEECCCCCCHHHHHHCCCHHHEEEEEECCCCCEEEEECHHHHHHHHHHHH
QKSSRYPGGLALRFARVLRYRDDKGPAEADTIDAVRALY
HHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SPSHAKLATVLLFDVATASADVGGTPSRLTKVARIADLLRRAAPNAALVAIVVSWLSGE
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCH
LRQRQIGVGWAALRSRPPAAAHPTLTVVAVDAAFAEIGAVAGKGAQARRAALLNALFAAA
HHHHHCCCCHHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TETEQTFLLRLLGGELRQGALAGIMADAVARAAGIPAAAVQRAAMLGGDLPAVAAAALSG
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCC
EASALDAFTLRVGRPVAPMLAQTAAGVAQAIERHGGQAIFEAKLDGARVQIHRAGDQVTV
CCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCEEEE
YTRSLDDVTARLPEVVTATLALPVEALIADGEAIALRPDNSPQRFQVTASRFGRSLDVAA
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEHHHCCCCHHHHH
AVAAQPLSVFIFDILHCDGIDLLDAPTTDRLAALDALVPPAQRVDRLLTADPDAAGRFLE
HHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHH
ATLAAGHEGVMAKAPGAPYQAGRRGAGWLKVKPVHTLDLVVLAVEWGSGRRRGKLSNIHL
HHHHCCCCCCEECCCCCCHHCCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEE
GARDPATGEFVMVGKTFKGMTDAMLDWQTARFTELAVGGTDGYVVRVRPEQVVEVAVDGV
CCCCCCCCCEEEECCCCCCHHHHHHCCCHHHEEEEEECCCCCEEEEECHHHHHHHHHHHH
QKSSRYPGGLALRFARVLRYRDDKGPAEADTIDAVRALY
HHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA