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The map label for this gene is gatA

Identifier: 41409143

GI number: 41409143

Start: 3387769

End: 3389250

Strand: Reverse

Name: gatA

Synonym: MAP3045c

Alternate gene names: 41409143

Gene position: 3389250-3387769 (Counterclockwise)

Preceding gene: 41409144

Following gene: 41409142

Centisome position: 70.17

GC content: 71.59

Gene sequence:

>1482_bases
GTGAACGAGATCATCCGGTCGGACGCCGCGACGCTGGCCGCCCGCATCGCCGCCAAGGAGCTGTCCTCGGTCGAGGTCAC
CCAGGCCTGCCTGGATCAGATCGCGGCGACCGACGAGCGCTACCACGCCTTTTTGCACGTTGCCGCCGACGAGGCGCTGG
GCGCGGCCGCGGTCGTCGACGAGGCGGTGGCGGCGGGCGAGCGGCTGCCCTCGCCGCTGGCCGGGGTCCCGCTGGCGCTC
AAGGACGTCTTCACCACCGTCGACATGCCCACCACCTGCGGGTCCAAGATTCTGCAGGGCTGGCGTTCGCCGTACGACGC
CACCGTCACCACGAAGCTGCGGGCCGCGGGCATCCCGATCCTGGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCCT
CGACCGAGAACTCGGCCTACGGCCCGACCCGCAATCCGTGGAATGTCGAGCGGGTGCCCGGCGGTTCCGGCGGCGGCAGC
GCGGCCGCGCTGGCGGCGTTCCAGGCGCCGCTGGCGATCGGCTCCGACACCGGCGGCTCGATCCGGCAGCCCGCCGCGCT
GACCGCGACCGTCGGGGTCAAGCCCACCTACGGCACGGTGTCCCGGTACGGCCTGGTGGCCTGCGCGTCATCGCTGGATC
AGGGCGGCCCGTGCGCGCGCACCGTACTCGACACCGCGCTGCTGCACCAGGTGATCGCCGGCCACGACATCCGCGACTCC
ACCTCCGTCGACGCGCCGGTGCCCGACGTCGTCGGCGCCGCCCGGGCCGGCACGGCCGGTGACCTCAAGGGTGTTCGGGT
CGGGGTGGTCAAGCAGCTGCGCGGCGAGGGATATCAGCCCGGCGTGCTGGCCTCCTTCGAGGCCGCCGTCGAGCAGTTGA
CCGCGCTGGGCGCCGAGGTCAGCGAGGTCGACTGCCCGCACTTCGAGTACGCGCTGGCCGCCTACTACCTGATTTTGCCC
TCGGAGGTGTCTAGCAACCTGGCCCGCTTCGACGCGATGCGCTACGGGCTGCGCATCGGCGACGACGGCAGCCACAGCGC
CGAGGAGGTGATGGCGCTGACCCGGGCCGCCGGTTTCGGCCCGGAGGTCAAGCGGCGCATCATGATCGGCACCTACGCGC
TGTCGGCCGGCTACTACGACGCCTATTACAACCAGGCCCAGAAGGTGCGCACGCTGATCGCCCGCGACCTCGACGCCGCC
TACGAGTCCGTCGACGTGGTGGTCTCGCCGGCCACCCCGACCACCGCGTTCGGGCTGGGGGAGAAGGTCGACGACCCGCT
GGCCATGTATTTGTTCGACCTGTGCACGCTGCCGCTGAACCTGGCCGGGAACTGCGGCATGTCGGTGCCATCCGGATTGT
CGCCCGACGACGACCTGCCGGTGGGGTTGCAGATCATGGCGCCCGCGCTGGCCGACGACCGGTTGTACCGGGTCGGCGCC
GCCTACGAGGCCGCGCGCGGCCCGCTGCGCAGCGCGATCTAA

Upstream 100 bases:

>100_bases
CGGACGAGCCGATGCCCTGCCTGACGCAGGCCGAAGCGCTGGCCGAAGCGCCCGAAGCCGTCGACGGCCGGTTCGCCGTC
CCGCAGATCCTGGGGGACGA

Downstream 100 bases:

>100_bases
GCAGCTCGCCATGAAGCCGCCGCCCCAGGCAAGATGAGGTCATGCGGATCGGAGTGCTCACCGGAGGCGGCGACTGCCCC
GGGCTCAACGCCGTCATCCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNEIIRSDAATLAARIAAKELSSVEVTQACLDQIAATDERYHAFLHVAADEALGAAAVVDEAVAAGERLPSPLAGVPLAL
KDVFTTVDMPTTCGSKILQGWRSPYDATVTTKLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVERVPGGSGGGS
AAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDIRDS
TSVDAPVPDVVGAARAGTAGDLKGVRVGVVKQLRGEGYQPGVLASFEAAVEQLTALGAEVSEVDCPHFEYALAAYYLILP
SEVSSNLARFDAMRYGLRIGDDGSHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAA
YESVDVVVSPATPTTAFGLGEKVDDPLAMYLFDLCTLPLNLAGNCGMSVPSGLSPDDDLPVGLQIMAPALADDRLYRVGA
AYEAARGPLRSAI

Sequences:

>Translated_493_residues
MNEIIRSDAATLAARIAAKELSSVEVTQACLDQIAATDERYHAFLHVAADEALGAAAVVDEAVAAGERLPSPLAGVPLAL
KDVFTTVDMPTTCGSKILQGWRSPYDATVTTKLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVERVPGGSGGGS
AAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDIRDS
TSVDAPVPDVVGAARAGTAGDLKGVRVGVVKQLRGEGYQPGVLASFEAAVEQLTALGAEVSEVDCPHFEYALAAYYLILP
SEVSSNLARFDAMRYGLRIGDDGSHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAA
YESVDVVVSPATPTTAFGLGEKVDDPLAMYLFDLCTLPLNLAGNCGMSVPSGLSPDDDLPVGLQIMAPALADDRLYRVGA
AYEAARGPLRSAI
>Mature_493_residues
MNEIIRSDAATLAARIAAKELSSVEVTQACLDQIAATDERYHAFLHVAADEALGAAAVVDEAVAAGERLPSPLAGVPLAL
KDVFTTVDMPTTCGSKILQGWRSPYDATVTTKLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVERVPGGSGGGS
AAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDIRDS
TSVDAPVPDVVGAARAGTAGDLKGVRVGVVKQLRGEGYQPGVLASFEAAVEQLTALGAEVSEVDCPHFEYALAAYYLILP
SEVSSNLARFDAMRYGLRIGDDGSHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAA
YESVDVVVSPATPTTAFGLGEKVDDPLAMYLFDLCTLPLNLAGNCGMSVPSGLSPDDDLPVGLQIMAPALADDRLYRVGA
AYEAARGPLRSAI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=36.7676767676768, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI195972892, Length=499, Percent_Identity=24.8496993987976, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=417, Percent_Identity=36.6906474820144, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17537465, Length=243, Percent_Identity=29.6296296296296, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=193, Percent_Identity=30.0518134715026, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=191, Percent_Identity=28.7958115183246, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=210, Percent_Identity=29.0476190476191, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=414, Percent_Identity=32.6086956521739, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319685, Length=421, Percent_Identity=30.4038004750594, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=201, Percent_Identity=31.3432835820896, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=38.2470119521912, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=25.1004016064257, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=25.1004016064257, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=25.1004016064257, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=25.1004016064257, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24644968, Length=522, Percent_Identity=25.6704980842912, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=203, Percent_Identity=32.512315270936, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648437, Length=203, Percent_Identity=32.512315270936, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550774, Length=203, Percent_Identity=32.512315270936, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356731, Length=254, Percent_Identity=28.3464566929134, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161078093, Length=518, Percent_Identity=25.2895752895753, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=144, Percent_Identity=35.4166666666667, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648439, Length=144, Percent_Identity=35.4166666666667, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51261; Mature: 51261

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIIRSDAATLAARIAAKELSSVEVTQACLDQIAATDERYHAFLHVAADEALGAAAVVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEHHHHHHHHHHHH
EAVAAGERLPSPLAGVPLALKDVFTTVDMPTTCGSKILQGWRSPYDATVTTKLRAAGIPI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEHHHCCCCE
LGKTNMDEFAMGSSTENSAYGPTRNPWNVERVPGGSGGGSAAALAAFQAPLAIGSDTGGS
ECCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEECCCCCCC
IRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDIRDS
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TSVDAPVPDVVGAARAGTAGDLKGVRVGVVKQLRGEGYQPGVLASFEAAVEQLTALGAEV
CCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCH
SEVDCPHFEYALAAYYLILPSEVSSNLARFDAMRYGLRIGDDGSHSAEEVMALTRAAGFG
HHCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCCC
PEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAAYESVDVVVSPATPTTAFGLG
HHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHCCC
EKVDDPLAMYLFDLCTLPLNLAGNCGMSVPSGLSPDDDLPVGLQIMAPALADDRLYRVGA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHHHHHH
AYEAARGPLRSAI
HHHHHCCCHHHCC
>Mature Secondary Structure
MNEIIRSDAATLAARIAAKELSSVEVTQACLDQIAATDERYHAFLHVAADEALGAAAVVD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEHHHHHHHHHHHH
EAVAAGERLPSPLAGVPLALKDVFTTVDMPTTCGSKILQGWRSPYDATVTTKLRAAGIPI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEHHHCCCCE
LGKTNMDEFAMGSSTENSAYGPTRNPWNVERVPGGSGGGSAAALAAFQAPLAIGSDTGGS
ECCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCEECCCCCCC
IRQPAALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHQVIAGHDIRDS
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
TSVDAPVPDVVGAARAGTAGDLKGVRVGVVKQLRGEGYQPGVLASFEAAVEQLTALGAEV
CCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCH
SEVDCPHFEYALAAYYLILPSEVSSNLARFDAMRYGLRIGDDGSHSAEEVMALTRAAGFG
HHCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHCCCC
PEVKRRIMIGTYALSAGYYDAYYNQAQKVRTLIARDLDAAYESVDVVVSPATPTTAFGLG
HHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHCCC
EKVDDPLAMYLFDLCTLPLNLAGNCGMSVPSGLSPDDDLPVGLQIMAPALADDRLYRVGA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHHHHHHH
AYEAARGPLRSAI
HHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]