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The map label for this gene is pfkA

Identifier: 41409142

GI number: 41409142

Start: 3386696

End: 3387727

Strand: Reverse

Name: pfkA

Synonym: MAP3044c

Alternate gene names: 41409142

Gene position: 3387727-3386696 (Counterclockwise)

Preceding gene: 41409143

Following gene: 41409141

Centisome position: 70.14

GC content: 68.31

Gene sequence:

>1032_bases
ATGCGGATCGGAGTGCTCACCGGAGGCGGCGACTGCCCCGGGCTCAACGCCGTCATCCGGGCGGTGGTGCGGACCTGCGA
CTCCCGGTACGGCTCGTCGGTGGTCGGATTCCAGGACGGCTGGCGCGGGTTGCTGGAGAACCGGCGCATCCAGTTGCACA
ACGACGACCGCAACGACCGGCTGCTGGCCAAGGGCGGGACCATGCTGGGCACCGCCCGCGTGCACCCGGACAAGTTGCGG
GCCGGGCTGAACCAGATCAAGCAGACCCTCGACGACAACGGGATCGACGTCCTCATCCCGATCGGCGGCGAAGGGACGCT
GACGGCCGCGCACTGGCTCTCCGAGGAGAACGTTCCCGTGGTCGGGGTGCCGAAGACCATCGACAACGACATCGATTGCA
CTGACGTGACTTTCGGCCACGACACCGCCTTGACCGTCGCCACCGACGCGATCGACCGCCTGCACAGCACGGCCGAATCG
CATCAACGCGTGATGTTGGTGGAGGTGATGGGCCGGCACGCCGGCTGGATCGCGCTCAACGCCGGACTGGCCTCCGGCGC
GCACATGACCCTGATCCCCGAGCAGCCCTTCGACGTCGAAGAAGTCTGCCGGCTCGTCAAACGTCGCTTCCAGCGCGGGG
ATTCGCATTTCATCTGCGTCGTCGCCGAGGGTGCCAAACCCATCCCCGGTTCGATTTCCTTGCGGGAAGGCGGGATTGAC
GAATTCGGCCATGAGCGCTTCACCGGGGTGGCCGCGCAGCTGGGTGCCGAGGTGGAGAAGCGGATCAACAAGGAGGTCCG
GGTGACGGTGCTGGGCCACGTGCAGCGCGGCGGCACCCCGACCGCCTACGACCGGGTGCTGGCCACCCGGTTCGGTGTGA
ACGCGGCCGACGCCGCGCACGCCGGGGAGTACGGCCAGATGGTGTCGCTGCGCGGGCAGGACATCGGACGGGTGCCGCTG
GCCGACGCGGTGCGTCAGCTCAAGCTGGTGCCCGAAAGCCGCTACGACGACGCCGCCGCGTTCTTCGGTTAG

Upstream 100 bases:

>100_bases
TGTACCGGGTCGGCGCCGCCTACGAGGCCGCGCGCGGCCCGCTGCGCAGCGCGATCTAAGCAGCTCGCCATGAAGCCGCC
GCCCCAGGCAAGATGAGGTC

Downstream 100 bases:

>100_bases
GCGGTCGGGCCCGCGGTCGGGCAAACAATCGGTGGCGACGAGTCAACCGCGGCCTGAATTCGCCGGCGAACGGTCCAGGT
TGACGCACTGACCGCCTATT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGLLENRRIQLHNDDRNDRLLAKGGTMLGTARVHPDKLR
AGLNQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATDAIDRLHSTAES
HQRVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGDSHFICVVAEGAKPIPGSISLREGGID
EFGHERFTGVAAQLGAEVEKRINKEVRVTVLGHVQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVSLRGQDIGRVPL
ADAVRQLKLVPESRYDDAAAFFG

Sequences:

>Translated_343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGLLENRRIQLHNDDRNDRLLAKGGTMLGTARVHPDKLR
AGLNQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATDAIDRLHSTAES
HQRVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGDSHFICVVAEGAKPIPGSISLREGGID
EFGHERFTGVAAQLGAEVEKRINKEVRVTVLGHVQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVSLRGQDIGRVPL
ADAVRQLKLVPESRYDDAAAFFG
>Mature_343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGLLENRRIQLHNDDRNDRLLAKGGTMLGTARVHPDKLR
AGLNQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATDAIDRLHSTAES
HQRVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGDSHFICVVAEGAKPIPGSISLREGGID
EFGHERFTGVAAQLGAEVEKRINKEVRVTVLGHVQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVSLRGQDIGRVPL
ADAVRQLKLVPESRYDDAAAFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=380, Percent_Identity=34.7368421052632, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI266453768, Length=375, Percent_Identity=36, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI266453748, Length=375, Percent_Identity=36, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4505749, Length=375, Percent_Identity=36, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI266453619, Length=375, Percent_Identity=36, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI48762920, Length=375, Percent_Identity=35.7333333333333, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1790350, Length=328, Percent_Identity=39.0243902439024, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI25147584, Length=366, Percent_Identity=32.5136612021858, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17558788, Length=381, Percent_Identity=32.0209973753281, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI72003601, Length=353, Percent_Identity=24.929178470255, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321679, Length=365, Percent_Identity=34.7945205479452, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323861, Length=372, Percent_Identity=34.9462365591398, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28573326, Length=375, Percent_Identity=34.9333333333333, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24652337, Length=375, Percent_Identity=35.2, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17647809, Length=375, Percent_Identity=35.2, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36991; Mature: 36991

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGLLENRRIQLHNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCCCCE
LLAKGGTMLGTARVHPDKLRAGLNQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSEENVPV
EEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHCCCCCCCE
VGVPKTIDNDIDCTDVTFGHDTALTVATDAIDRLHSTAESHQRVMLVEVMGRHAGWIALN
EECCHHCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHCEEEEEEHHCCCCCEEEEE
AGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGDSHFICVVAEGAKPIPGSISLREGGID
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCC
EFGHERFTGVAAQLGAEVEKRINKEVRVTVLGHVQRGGTPTAYDRVLATRFGVNAADAAH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHC
AGEYGQMVSLRGQDIGRVPLADAVRQLKLVPESRYDDAAAFFG
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTCDSRYGSSVVGFQDGWRGLLENRRIQLHNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEECCCCCCE
LLAKGGTMLGTARVHPDKLRAGLNQIKQTLDDNGIDVLIPIGGEGTLTAAHWLSEENVPV
EEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHCCCCCCCE
VGVPKTIDNDIDCTDVTFGHDTALTVATDAIDRLHSTAESHQRVMLVEVMGRHAGWIALN
EECCHHCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHHHHCEEEEEEHHCCCCCEEEEE
AGLASGAHMTLIPEQPFDVEEVCRLVKRRFQRGDSHFICVVAEGAKPIPGSISLREGGID
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCC
EFGHERFTGVAAQLGAEVEKRINKEVRVTVLGHVQRGGTPTAYDRVLATRFGVNAADAAH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHC
AGEYGQMVSLRGQDIGRVPLADAVRQLKLVPESRYDDAAAFFG
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]