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The map label for this gene is ilvB_1

Identifier: 41409136

GI number: 41409136

Start: 3379032

End: 3380900

Strand: Reverse

Name: ilvB_1

Synonym: MAP3038c

Alternate gene names: 41409136

Gene position: 3380900-3379032 (Counterclockwise)

Preceding gene: 41409137

Following gene: 41409135

Centisome position: 70.0

GC content: 69.5

Gene sequence:

>1869_bases
GTGAGCGCTCCCACCAGGCGGCCCGCCCCCGACGCACCCGGCGCTGCCGGCATCGCCCCGGCCCCGCCGGCCCCGGCCGC
GAAGCCCGCCGCCGGCAAACCGAAACGCATTGGGCCCGAACAAGTTACCGGCGCGCAATCGGTGATCCGATCGCTGGAGG
AGCTCGGCGTCGAGGTGATCTTCGGCATTCCCGGCGGCGCGGTACTGCCGGTCTATGACCCGCTGTTCGACTCCAAGAAG
CTGCGGCACGTGCTGGTCCGCCACGAGCAGGGCGCCGGTCACGCCGCCAGCGGCTACGCGCACGCCACCGGCAAGGTCGG
TGTCTGCATGGCCACCTCCGGTCCGGGCGCCACCAACCTGGTCACCGCGCTGGCCGACGCCCAGATGGACTCGATTCCCG
TCGTCGCCGTCACCGGCCAGGTGGGACGCACGCTAATCGGCACCGACGCCTTCCAGGAGGCCGACATCTCCGGCATCACC
ATGCCGATCACCAAGCACAACTTCCTGGTTCGCTCCGGCAACGAGATCCCGCAGGTGCTTGCCGAGGCGTTCCACATCGC
CGCCTCCGGCCGCCCCGGCGCGGTGCTCGTCGACATCCCCAAGGACGTGCTGCAGGGCCAGTGCACGTTCAGCTGGCCGC
CGCGCATCCACCTGCCCGGGTACAAGCCGACCACCAAACCGCACAGCCGGCAGATCCGCGAAGCCGCCAAGCTGATCGCG
GCCGCCCGCAAACCGGTGCTGTACGTGGGCGGCGGTGTCATCCGCGGGGAGGCCAGCGAGCAGCTGCGCGAACTGGCCGA
GCTGACCGGCATCCCGGTGGTGACCACGCTGATGGCCCGCGGCGCGTTCCCGGACAGCCACCGTCAGCACCTGGGCATGC
CCGGGATGCACGGCACCGTCGCGGCGGTGGCGGCCCTGCAGCGCAGCGACCTGCTGATCGCGCTGGGTACGCGGTTCGAT
GACCGGGTGACCGGCAAGCTGGACACCTTCGCCCCGGAAGCCAAGGTCATCCACGCCGACATCGACCCGGCCGAGATCGG
CAAGAACCGGCATGCCGACGTGCCGATCGTCGGCGACGTCAAGGCCGTCATCGCCGAACTGGTCGAGATGCTGCGCCACG
ACGGGGCGCCCGGCAATCTCGACATCGCCGACTGGTGGGCCTACCTCGACGACGTCCAGTCCACCTATCCGTTGAGCTAC
GGGCCGCAGAGCGACGGCAGCCTCGGGCCCGAGTACGTCATCGAGAAGCTGGGCCAGATCGCTGGCCCCGACGCGGTGTA
CGTCGCCGGGGTGGGCCAGCACCAGATGTGGGCGGCGCAATTCATCTCCTACGAGAAGCCGCGCACCTGGCTCAACTCCG
GCGGCCTGGGCACCATGGGGTTCGCCATTCCGGCCGCCATGGGGGCCAAGATGGCTCGCCCGGAGGCCGAGGTGTGGGCG
ATCGACGGCGACGGCTGTTTCCAGATGACCAACCAGGAACTGGCCACCTGCGCCGTCGAGGGCATTCCGATCAAGGTGGC
GCTGATCAACAACGGCAACCTGGGCATGGTGCGCCAGTGGCAGACCCTGTTCTACGAAGAGCGGTATTCGCAAACGGATC
TGGCCACCCACTCGCACCGCATCCCGGACTTCGTCAAGCTCGCCGAGGCGCTGGGCTGCGTCGGATTGCGTTGCGAGCGT
GAGGAAGACGTCGTCGACGTGATCAACGCTGCCCGGGCGATCAACGACCGGCCGGTGGTGATCGACTTCATCGTCGGCGC
CGACGCGCAGGTGTGGCCGATGGTGGCCGCCGGCACCAGCAACGACGAGATCCAGGCCGCCCGCGGGATCCGCCCGCTGT
TCGACGACGAGACCGAAGGGCACGCCTGA

Upstream 100 bases:

>100_bases
AAGCAATCCAGCACCAACGCGCAACCCTCGTGCAGCACAAGCTGTCGGGGGTTTTTTGTTGCCCCGCGAGACTCCCAGAG
TGAATAAGGACTGATCGACA

Downstream 100 bases:

>100_bases
TGAGTCCGCAGACGCACACGCTGTCGGTGCTGGTCGAAGACAAACCGGGTGTGCTCGCCCGGGTGGCGGCGCTGTTCAGC
CGGCGCGGTTTCAACATCGA

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MSAPTRRPAPDAPGAAGIAPAPPAPAAKPAAGKPKRIGPEQVTGAQSVIRSLEELGVEVIFGIPGGAVLPVYDPLFDSKK
LRHVLVRHEQGAGHAASGYAHATGKVGVCMATSGPGATNLVTALADAQMDSIPVVAVTGQVGRTLIGTDAFQEADISGIT
MPITKHNFLVRSGNEIPQVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRIHLPGYKPTTKPHSRQIREAAKLIA
AARKPVLYVGGGVIRGEASEQLRELAELTGIPVVTTLMARGAFPDSHRQHLGMPGMHGTVAAVAALQRSDLLIALGTRFD
DRVTGKLDTFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVIAELVEMLRHDGAPGNLDIADWWAYLDDVQSTYPLSY
GPQSDGSLGPEYVIEKLGQIAGPDAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKMARPEAEVWA
IDGDGCFQMTNQELATCAVEGIPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSHRIPDFVKLAEALGCVGLRCER
EEDVVDVINAARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDETEGHA

Sequences:

>Translated_622_residues
MSAPTRRPAPDAPGAAGIAPAPPAPAAKPAAGKPKRIGPEQVTGAQSVIRSLEELGVEVIFGIPGGAVLPVYDPLFDSKK
LRHVLVRHEQGAGHAASGYAHATGKVGVCMATSGPGATNLVTALADAQMDSIPVVAVTGQVGRTLIGTDAFQEADISGIT
MPITKHNFLVRSGNEIPQVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRIHLPGYKPTTKPHSRQIREAAKLIA
AARKPVLYVGGGVIRGEASEQLRELAELTGIPVVTTLMARGAFPDSHRQHLGMPGMHGTVAAVAALQRSDLLIALGTRFD
DRVTGKLDTFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVIAELVEMLRHDGAPGNLDIADWWAYLDDVQSTYPLSY
GPQSDGSLGPEYVIEKLGQIAGPDAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKMARPEAEVWA
IDGDGCFQMTNQELATCAVEGIPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSHRIPDFVKLAEALGCVGLRCER
EEDVVDVINAARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDETEGHA
>Mature_621_residues
SAPTRRPAPDAPGAAGIAPAPPAPAAKPAAGKPKRIGPEQVTGAQSVIRSLEELGVEVIFGIPGGAVLPVYDPLFDSKKL
RHVLVRHEQGAGHAASGYAHATGKVGVCMATSGPGATNLVTALADAQMDSIPVVAVTGQVGRTLIGTDAFQEADISGITM
PITKHNFLVRSGNEIPQVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRIHLPGYKPTTKPHSRQIREAAKLIAA
ARKPVLYVGGGVIRGEASEQLRELAELTGIPVVTTLMARGAFPDSHRQHLGMPGMHGTVAAVAALQRSDLLIALGTRFDD
RVTGKLDTFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVIAELVEMLRHDGAPGNLDIADWWAYLDDVQSTYPLSYG
PQSDGSLGPEYVIEKLGQIAGPDAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKMARPEAEVWAI
DGDGCFQMTNQELATCAVEGIPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSHRIPDFVKLAEALGCVGLRCERE
EDVVDVINAARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDETEGHA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=28.0210157618214, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI21361361, Length=593, Percent_Identity=27.6559865092749, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI87081685, Length=579, Percent_Identity=46.9775474956822, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=44.8154657293497, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=545, Percent_Identity=37.7981651376147, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=29.3594306049822, Blast_Score=209, Evalue=6e-55,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=25.8527827648115, Blast_Score=163, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=564, Percent_Identity=25.709219858156, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=573, Percent_Identity=25.8289703315881, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=524, Percent_Identity=28.8167938931298, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=611, Percent_Identity=42.8805237315876, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=23.7704918032787, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=502, Percent_Identity=21.7131474103586, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=498, Percent_Identity=21.8875502008032, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=501, Percent_Identity=21.3572854291417, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=27.1276595744681, Blast_Score=192, Evalue=5e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65975; Mature: 65844

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTRRPAPDAPGAAGIAPAPPAPAAKPAAGKPKRIGPEQVTGAQSVIRSLEELGVEVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEE
FGIPGGAVLPVYDPLFDSKKLRHVLVRHEQGAGHAASGYAHATGKVGVCMATSGPGATNL
EECCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEECCCCCHHHH
VTALADAQMDSIPVVAVTGQVGRTLIGTDAFQEADISGITMPITKHNFLVRSGNEIPQVL
HHHHHHCCCCCCCEEEEECCCCCEEECCCCHHHCCCCCEEEEEECCCEEEECCCHHHHHH
AEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRIHLPGYKPTTKPHSRQIREAAKLIA
HHHHHHHCCCCCCEEEEECCHHHHCCCEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHH
AARKPVLYVGGGVIRGEASEQLRELAELTGIPVVTTLMARGAFPDSHRQHLGMPGMHGTV
HHCCCEEEECCEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCCCCCCHHH
AAVAALQRSDLLIALGTRFDDRVTGKLDTFAPEAKVIHADIDPAEIGKNRHADVPIVGDV
HHHHHHHHCCEEEEECCCCCCCCCCCHHCCCCCCEEEEECCCHHHCCCCCCCCCCEECCH
KAVIAELVEMLRHDGAPGNLDIADWWAYLDDVQSTYPLSYGPQSDGSLGPEYVIEKLGQI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
AGPDAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKMARPEAEVWA
CCCCEEEEEECCCHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
IDGDGCFQMTNQELATCAVEGIPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSHR
ECCCCCEEECCHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHCCC
IPDFVKLAEALGCVGLRCEREEDVVDVINAARAINDRPVVIDFIVGADAQVWPMVAAGTS
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCC
NDEIQAARGIRPLFDDETEGHA
CCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPTRRPAPDAPGAAGIAPAPPAPAAKPAAGKPKRIGPEQVTGAQSVIRSLEELGVEVI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEE
FGIPGGAVLPVYDPLFDSKKLRHVLVRHEQGAGHAASGYAHATGKVGVCMATSGPGATNL
EECCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEECCCCEEEEEECCCCCHHHH
VTALADAQMDSIPVVAVTGQVGRTLIGTDAFQEADISGITMPITKHNFLVRSGNEIPQVL
HHHHHHCCCCCCCEEEEECCCCCEEECCCCHHHCCCCCEEEEEECCCEEEECCCHHHHHH
AEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRIHLPGYKPTTKPHSRQIREAAKLIA
HHHHHHHCCCCCCEEEEECCHHHHCCCEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHH
AARKPVLYVGGGVIRGEASEQLRELAELTGIPVVTTLMARGAFPDSHRQHLGMPGMHGTV
HHCCCEEEECCEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCCCCCCHHH
AAVAALQRSDLLIALGTRFDDRVTGKLDTFAPEAKVIHADIDPAEIGKNRHADVPIVGDV
HHHHHHHHCCEEEEECCCCCCCCCCCHHCCCCCCEEEEECCCHHHCCCCCCCCCCEECCH
KAVIAELVEMLRHDGAPGNLDIADWWAYLDDVQSTYPLSYGPQSDGSLGPEYVIEKLGQI
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
AGPDAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKMARPEAEVWA
CCCCEEEEEECCCHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
IDGDGCFQMTNQELATCAVEGIPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSHR
ECCCCCEEECCHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHCCC
IPDFVKLAEALGCVGLRCEREEDVVDVINAARAINDRPVVIDFIVGADAQVWPMVAAGTS
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCC
NDEIQAARGIRPLFDDETEGHA
CCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921849 [H]